Genome size estimation and characterization of Sinosenecio oldhamianus

doi:10.14088/j.cnki.issn0439-8114.2026.05.032

Abstract

Abstract: To determine the genome size and sequence composition characteristics of the medicinal plant Sinosenecio oldhamianus and to promote the development and utilization of its genome genetic resources, flow cytometry was used with Nicotiana benthamiana as a reference to estimate the genome size of Sinosenecio oldhamianus. Based on the Illumina NovaSeq 6000 platform, genome survey sequencing was conducted, K-mer analysis was performed on the sequencing data to evaluate genome heterozygosity, GC content, and repetitive sequence proportion, and sequence similarity comparison was carried out through the NCBI NT database. Flow cytometry measurement results showed that the genome size of Sinosenecio oldhamianus was approximately 1 620 Mb. Based on Illumina platform sequencing, a total of 122.10 Gb of high-quality data was obtained, with an average sequencing depth of 65×. Statistical analysis of the 19-mer frequency distribution of the Sinosenecio oldhamianus genome showed that its genome size was approximately 1 725 Mb, GC content was 38.66%, and the overall distribution was uniform without obvious bias, indicating that the sequencing data quality was reliable and there was no significant exogenous contamination. The heterozygosity of the Sinosenecio oldhamianus genome was as high as 12.10%, and the repetitive sequence proportion was 49.20%, indicating that it belonged to a typical complex genome type with high heterozygosity and high repetitive sequence content. The NCBI NT database comparison results showed that the sequences of Sinosenecio oldhamianus had the highest matching rate with the reference sequence of Pulicaria dysenterica, followed by Gynoxys mandonii, Sinosenecio oldhamianus, and Morina longifolia.

Key words: Sinosenecio oldhamianus, Nicotiana benthamiana, flow cytometry, K-mer analysis, genome size, medicinal plant, characterization

CLC Number:

S567.23

ZHANG Peng, ZHANG Ze-zhi, YOU Lei, ZHENG Lan-lan, ZHANG Yong-hong. Genome size estimation and characterization of Sinosenecio oldhamianus[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(5): 214-220.

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