采收期对费约果活性氧代谢及AsA-GSH循环的影响

doi:10.14088/j.cnki.issn0439-8114.2018.11.012

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (11): 50-54.doi: 10.14088/j.cnki.issn0439-8114.2018.11.012

采收期对费约果活性氧代谢及AsA-GSH循环的影响

陆云梅, 黄仁华

西南科技大学生命科学与工程学院,四川绵阳 621010

收稿日期:2017-12-17 出版日期:2018-06-10 发布日期:2019-12-19
通讯作者: 黄仁华（1979-）,男,副教授,主要从事园艺产品贮藏加工研究,（电话）13881147717（电子信箱）huangrenhua@swust.edu.cn。
作者简介:陆云梅（1979-）,女（苗族）,贵州长顺人,讲师,硕士,主要从事园艺产品贮藏加工研究,（电话）13990150586（电子信箱）59736201@qq.com
基金资助:
四川省教育厅技术研究项目（17zd1126）; 西南科技大学生物质资源平台研究项目（14tdgc01）; 西南科技大学龙山学术人才科研支持计划项目（17LZX633）; 西南科技大学校基金项目（17GJZX04）

Effects of Harvesting Time on the Active Oxygen Metabolism and AsA-GSH Cyclein Feijoa sellowiana Pulp

LU Yun-mei, HUANG Ren-hua

College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan, China

Received:2017-12-17 Online:2018-06-10 Published:2019-12-19

摘要/Abstract

摘要： 为了阐明采收期对费约果贮藏期间活性氧代谢的影响,确定果实最佳采收时期,以品种Coolidge果实为材料,分析了始熟期（花后130 d）、盛熟期（花后150 d）和过熟期（花后170 d）采收果实在贮藏期间果肉线粒体细胞活性氧代谢及抗坏血酸—谷胱甘肽（AsA-GSH）循环的差异。结果显示,盛熟期采收的果实在贮藏后期H₂O₂含量和O₂^-·产生速率显著高于始熟期和过熟期采收的果实,而贮藏前6 d无显著差异;同时,果实在贮藏前期,抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）和谷胱甘肽还原酶（GR）等酶活性以及抗坏血酸（AsA）和谷胱甘肽（GSH）含量变化较小,但9 d后呈迅速增加的变化趋势,其中DHAR、GR活性以及AsA和GSH含量增加速率以过熟期采收的果实最大,其次为始熟期和盛熟期。因此,针对果实贮藏期活性氧代谢及AsA-GSH循环而言,采收时间对费约果短期贮藏无显著影响,而中长期贮藏以盛熟期（花后150 d）采收为最佳时期。

关键词: 采收期, 费约果, 线粒体, 活性氧代谢, AsA-GSH循环

Abstract: In order to elucidate the effects of harvest time on active oxygen metabolism of Feijoa sellowiana fruit during storage and determine the best harvest time,the active oxygen metabolism and AsA-GSH cycle in mitochondria of Feijoa sellowiana pulp harvested at initial ripe stage （IRS,130 d after flowering）,full ripe stage （FRS,150 d after flowering） and excess ripe stage（ERS,170 d after flowering） were studied during the storage. The results showed that the H₂O₂ content and O₂^-· production rate in FRS fruits was significantly higher than those in IRS and ERS fruits during the late storage stage,but there were no significant difference before 6 d storage. At the same time,there were no significant changes in APX,DHAR,GR activities and AsA and GSH contents during the early stage of postharvest storage. After storage 9 d,the rapidly increasing trend in these indexes were showed and the biggest increasing rate was found in ERS fruits,followed by ERS fruits and FRS frruits. Therefore,in the case of active oxygen metabolism and AsA-GSH cycle during the fruit storage period,harvesting time had no significant difference on the F. sellowiana fruits in short-term storage,but for long-term storage,the full ripe stage（150 d after anthesis） was optimal harvesting time.

Key words: harvesting time, Feijoa sellowiana, mitochondria, active oxygen metabolism, AsA-GSH cycle

中图分类号:

S667.9

陆云梅, 黄仁华. 采收期对费约果活性氧代谢及AsA-GSH循环的影响[J]. 湖北农业科学, 2018, 57(11): 50-54.

LU Yun-mei, HUANG Ren-hua. Effects of Harvesting Time on the Active Oxygen Metabolism and AsA-GSH Cyclein Feijoa sellowiana Pulp[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(11): 50-54.

参考文献

[1] DAVID BATEMAN L T D. Feijoas-Origins, Cultivation and uses[M].New Zealand:The Horticucture and Food Research Institute of New Zealand Ltd,2002,7-79.
[2] 王丹,刘仁道,任少雄.食用、观赏兼用果树新种类费约果引种的气候适应性分析[J].中国南方果树,2007,36(6):39-41.
[3] WESTON R J.Bioactive products from fruit of the feijoa(Feijoa sellowiana,Myrtaceae):A review[J].Food Chemistry,2010,121:923-926.
[4] SAJ O P,ROY R K,SAVITHA S V.Chemical composition and antimicrobial properties of essential oil of Feijoa sellowiana O. Berg. (pineapple guava)[J]. Journal of Pure and Applied Microbiology,2008,2(1):227-230.
[5] FERNANDEZ X,LOISEAU A M, POULAIN S,et al.Chemical composition of the essential oil from Feijoa(Feijoa sellowiana Berg.) peel[J].Journal of Essential Oil Research,2004,16(3): 274-275.
[6] 孙芳娟,韩明玉,赵彩萍,等.不同采收成熟度油桃贮藏效果及果肉细胞超微结构观察[J].果树学报,2009,26(4):450-455.
[7] 程顺昌,冷俊颖,任小林,等.不同环丙烯类乙烯抑制剂对苹果常温贮藏保鲜效果的影响[J].农业工程学报,2012,28(6):269-273.
[8] 金昌海,索标,阚娟,等.桃果实采后贮藏过程中细胞壁多糖的变化[J].植物生理与分子生物学学报,2006,32(6):657-664.
[9] 齐秀东,魏建梅,高海生,等.梨果实发育软化与果胶多糖降解特性的关系[J].中国农业科学,2015,48(15):3027-3037.
[10] 蔡琦,玮张燕,田元元,等.二氧化氯对蓝莓果实品质及活性氧代谢影响的多变量分析[J].中国食品学报,2015,15(9):231-239.
[11] 张忠,马朝玲,丁若珺,等.丁若珺采后乙烯利处理对软儿梨果实后熟期生理及品质的影响[J].食品科学,2017,38(21):252-258
[12] VAN EMMERIK W,WAGNER A M,VAN DER L H W. A quantitative comparison of respirat ion in cells and mitochondria from Petunia hybrida suspension culture[J]. J Plant Physiol,1992,139:390-396.
[13] 林植芳,李双顺,林桂珠,等.衰老叶片和叶绿体中H₂O₂的累积与膜脂过氧化的关系[J].植物生理学报,1988,14(1):16-22.
[14] 王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,16(6):55-57.
[15] ARAKAWA N,TSUTSUMI K,SANCEDA N G.A rapid and sensitive method for the determination of ascorbic acid using 4,7-diphenyl-1,10-phenanthroline[J]. Agric Biol Chem,1981, 45:1289-1290.
[16] NAKAGAWARA S,SAGISAKA S.Increase in enzyme activities related to ascorbate metabolism during cold acclimation of poplar twigs[J]. Plant Cell Physiol,1984,25:899-906.
[17] CASTILLO F J,GREPPIN H.Extracellular ascorbic acid and enzyme activities related to ascorbic acid metabolism in Sedum album L. leaves after ozone exposure[J].Environ Exp Bot,1988, 28:232-233.
[18] SHIGEOKA S,NAKANO Y,KITAOKA S.Metabolism of hydrogen peroxide in Euglena gracilis z by L-ascorbic acid peroxidase[J].Biochem,1980,186:377-380.
[19] SMITH I K,VIERHELLER,T L,THORNE C A.Assay of glutathione reductase in crude tissue homogenates using 5,5-dithione(2-nitrobenzoic acid)[J]. Anal Biochem,1988,175:408-413.
[20] KAN J,WANG H M,JIN C H.Changes of reactive oxygen species and related enzymes in mitochondrial respiration during storage of harvested peach fruits[J]. Agricultural Sciences in China,2011,10(1):149-158.
[21] AHMADI-AFZADIA M,TAHIRB I,NYBOMA H.Impact of harvesting time and fruit firmness on the tolerance to fungal storage diseases in an apple germplasm collection[J]. Postharvest Biology and Technology,2013,82:51-58.
[22] SALA J M,LAFUENTE M T.Antioxidant enzymes activities and rindstaining in ‘Navelina’ oranges as affected by storage relative humidity and ethylene conditioning[J]. Postharvest Biology and Technology,2004,31:277-285.
[23] ZIEGLER D M.Role of reversible oxidation-reduction of enzyme thiols-disulfides in metabolic regulation[J]. Annu Rev Biochem,1985,54:305-329.

采收期对费约果活性氧代谢及AsA-GSH循环的影响

Effects of Harvesting Time on the Active Oxygen Metabolism and AsA-GSH Cyclein Feijoa sellowiana Pulp

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