生防因子间型假丝酵母C410液体酵母保存研究

doi:10.14088/j.cnki.issn0439-8114.2018.16.015

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (16): 66-69.doi: 10.14088/j.cnki.issn0439-8114.2018.16.015

生防因子间型假丝酵母C410液体酵母保存研究

李若晨, 张静, 杨龙, 李国庆

华中农业大学植物科学技术学院,武汉 430070

收稿日期:2018-03-30 发布日期:2019-12-16
通讯作者: 李国庆，教授，博士，主要从事植物病害生物防治研究，（电子信箱）guoqingli@mail.hzau.edu.cn。
作者简介:李若晨（1990-）,浙江金华人,助理农艺师,硕士,主要从事农业技术推广工作,（电话）13819927649（电子信箱）lrcyes@webmail.hzau.edu.cn;
基金资助:
国家公益性行业（农业）科研专项（201303025）

Preservation of Liquid Formulation of the Biocontrol Agent Candida intermedia C410

LI Ruo-chen, ZHANG Jing, YANG Long, LI Guo-qing

College of Plant Science & Technology,Huazhong Agricultural University,Wuhan 430070,China

Received:2018-03-30 Published:2019-12-16

摘要/Abstract

摘要： 对生防菌间型假丝酵母（Candida intermedia）C410菌株的液体酵母保存方法进行研究。结果表明,当保存起始细胞浓度为2×10⁹个细胞/mL,4 ℃恒温保存,在磷酸缓冲液PPB中添加5%海藻糖和1 mmol/L维生素C时,能够使C410酵母细胞保持高效活力持续90 d以上。对保存90 d的液体酵母防效进行测定,发现其防治效果与新鲜酵母细胞悬浮液的防效相当,都能抑制番茄灰霉病菌的生长繁殖,保持番茄果实品质完整。

关键词: 灰霉病, 生物防治, 间型假丝酵母（Candida intermedia）C410, 液体酵母

Abstract: The preservation of liquid formulation of the biocontrol agent Candida intermedia C410 was primarily explored. Results showed that, while the initial preserving concentration was 2×10⁹ cells/mL, storage temperature was 4 ℃, liquid formulation of C410 with high viability of cells were phosphate buffers supplemented with 5% trehalose and 1 mmol/L ascorbic acid. The liquid formulation kept high viability of yeast cells for more than 90 days. Suspensions of liquid formulation after 90 days and fresh yeast cells were tested against B. cinerea on tomato fruits, both suspensions exhibited prospective biocontrol effect, gray mould of tomato was inhibited while the quality of tomato fruits maintain completely good.

Key words: gray mold, biocontrol, Candida intermedia C410, liquid formulation

中图分类号:

S436.412.1⁺3

李若晨, 张静, 杨龙, 李国庆. 生防因子间型假丝酵母C410液体酵母保存研究[J]. 湖北农业科学, 2018, 57(16): 66-69.

LI Ruo-chen, ZHANG Jing, YANG Long, LI Guo-qing. Preservation of Liquid Formulation of the Biocontrol Agent Candida intermedia C410[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(16): 66-69.

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生防因子间型假丝酵母C410液体酵母保存研究

Preservation of Liquid Formulation of the Biocontrol Agent Candida intermedia C410

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