湖北农业科学 ›› 2018, Vol. 57 ›› Issue (20): 89-92.doi: 10.14088/j.cnki.issn0439-8114.2018.20.020

• 植物保护 • 上一篇    下一篇

狗芽根用于除草剂先导化合物筛选的研究

张志刚, 王开梅, 吴兆圆, 万中义, 张遵霞   

  1. 湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药分中心
  • 收稿日期:2018-07-10 发布日期:2018-10-25
  • 通讯作者: 王开梅(1969-),男,湖北武汉人,研究员,硕士,主要从事微生物农药研究,(电话)18627065137(电子信箱)wang_kaimei@163.com。
  • 作者简介:张志刚(1971-),男,湖北天门人,农艺师,主要从事微生物农药研究,(电话)13638694161(电子信箱)yilu98@163.com
  • 基金资助:
    国家重点研发计划重点专项(2017YFD0201301-4); 湖北省农业科技创新中心创新资金项目(2016-620-000-001-039)

Study on Cynodon dactylon for Screening of Lead Compounds of Herbicide from Microorganism

ZHANG Zhi-gang, WANG Kai-mei, WU Zhao-yuan, WAN Zhong-yi, ZHANG Zun-xia   

  1. 1.Hubei Biopesticide Engineering Research Center/The Branch Center of Biopesiticide,Hubei Agricultural Scientific and Technological Innovation Center
  • Received:2018-07-10 Published:2018-10-25

摘要: 以水琼脂表面涂布法对早熟禾(Poa annua L.)和狗芽根[Cynodon dactylon (L.) Pers.]进行筛选敏感度和抗真菌能力的比较。测试了狗芽根种子在温度、培养基量、生长时期3种不同条件下对标准化合物的敏感度。结果表明,狗芽根比早熟禾对除草剂标准化合物更敏感,且有更强的抗真菌污染能力。狗芽根种子在25 ℃的条件下,对除草剂标准化合物的敏感度最高;减少水琼脂用量,即提高样品的终浓度,等同于提高狗芽根筛选的敏感度;狗芽根种子培养48 h后,对标准化合物敏感度最高。对1 880个微生物源提取物进行筛选,表明狗芽根作为单子叶杂草,用于先导化合物筛选具有稳定、高效、敏感度高等突出优点;狗芽根是目前单子叶除草剂先导化合物筛选的首选靶标。

关键词: 早熟禾(Poa annua L.), 狗芽根[Cynodon dactylon (L.) Pers.], 先导化合物, 除草活性, 单子叶植物

Abstract: The screening sensitivity and antifungal contamination ability of Poa pratensis and Cynodon dactylon under artificial conditions were compared. C. dactylon had been assayed for their responses to 2 herbicide compounds under different temperature,amount of agar-solution and growth period. The results showed that C. dactylon was more sensitive to herbicide standard compounds and also stronger in antifungal ability than P. pratensis. C. dactylon showed the highest sensitivity at 25 ℃. C. dactylon showed the most sensitive to standard compound after 48 h incubation,reducing the amount of agar-solution could improve the final concentration of the compounds,it was equivalent to increasing the sensitivity of the C. dactylon screening. The screening of 1 880 real extracts showed that C. dactylon was stable,high efficiency,and high sensitivity target of monocotyledon weed for the screening. C. dactylon was one of the top choices of monocotyledon for screening of herbicide lead compounds.

Key words: Poa pratensis L., Cynodon dactylon (L.) Pers., lead compound, herbicidal activity, monocotyledon

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