湖北农业科学 ›› 2018, Vol. 57 ›› Issue (24): 81-83.doi: 10.14088/j.cnki.issn0439-8114.2018.24.022

• 植物保护 • 上一篇    下一篇

里氏木霉发酵培养基的响应面法优化

饶犇, 周荣华, 闵勇, 廖先清, 刘芳, 陈伟, 龙同, 张光阳   

  1. 湖北省生物农药工程研究中心,武汉 430064
  • 收稿日期:2018-06-21 出版日期:2018-12-25 发布日期:2020-04-01
  • 通讯作者: 龙 同。
  • 作者简介:饶 犇(1983-),男,湖北孝感人,助理研究员,博士,主要从事发酵工程方面的研究,(电话)15623076968(电子信箱)623253512@qq.com
  • 基金资助:
    湖北省农业科学院重大研发成果培育专项(2017CGPY01); 国家自然科学基金项目(31501695); 湖北省技术创新专项重大项目(2016ABA103); 国家重点研发计划项目(2017YFD0200900)

Optimizing on Fermentation Conditions of Trichoder mareesei by Response Surface Analysis Methodology

RAO Ben, ZHOU Rong-hua, MIN Yong, LIAO Xian-qing, LIU Fang, CHEN Wei, LONG Tong, ZHANG Guang-yang   

  1. National Biopesticide Engineering Technology Research Center, Wuhan 430064,China
  • Received:2018-06-21 Online:2018-12-25 Published:2020-04-01

摘要: 为了得到里氏木霉(Trichoder mareesei)发酵的最佳培养基,应用Plackett-Burman设计法对影响里氏菌丝体产量的关键因子进行了筛选,并进一步采用响应面分析法对影响菌丝产量的关键因素最佳水平进行了研究。结果表明,影响里氏木霉菌丝体产量的关键因素为鱼蛋白胨和甘油的浓度。通过响应面分析法的拟合和推算,得到在鱼蛋白胨和甘油浓度分别为1.414 3和2.439 3 g/100 mL时模型预测发酵最佳产量为85 g/100 mL,验证值为81 g/100 mL,预测值与验证值吻合较好,约是原始培养基产量(60 g/100 mL)的1.35倍。

关键词: 里氏木霉(Trichoder mareesei), Plackett-Burman设计法, 响应面分析法

Abstract: In order to obtain the optimal medium composition for Trichoder mareesei production, the Plackett-Burman design was used to evaluate the effects of variables, and the optimum levels of key factors affecting mycelium yield were conducted by response surface analysis methodology. The results showed that, the optimal values of the two parameters determined by response surface analysis methodology were fish peptone 1.414 3 g/100 mL and glycerol 2.439 3 g/100 mL. At this point,the model predicted optimal fermentation yield was 85 g/100 mL, and the verified value was 81 g/100 mL,and they were in agreement and it was about 1.35 times the yield of the original medium (60 g/100 mL).

Key words: Trichoder mareesei, Plackett-Burman design, response surface analysis methodology

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