湖北农业科学 ›› 2019, Vol. 58 ›› Issue (12): 124-129.doi: 10.14088/j.cnki.issn0439-8114.2019.12.029

• 畜牧·兽医 • 上一篇    下一篇

不同渥堆程度青砖茶改善小鼠胃肠道功能的研究

唐飞1, 张岳峰2, 曲凤凤1, 陈玉琼1, 余志1, 倪德江1   

  1. 1.园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院茶学系,武汉 430070;
    2.武汉黄鹤楼茶叶有限公司,武汉 430032
  • 收稿日期:2018-08-16 出版日期:2019-06-25 发布日期:2019-12-05
  • 通讯作者: 倪德江(1966-),男,教授,博士,主要从事茶叶加工与功能化学研究,(电子信箱)nidj@mail.hzau.edu.cn。
  • 作者简介:唐 飞(1989-),男,湖南邵阳人,硕士研究生,研究方向为茶叶加工与功能化学,(电话)18773349491(电子信箱)84990373@qq.com。
  • 基金资助:
    国家自然科学基金项目(31270732)

Effect of fermentation level of dark brick tea on improving gastrointestinal function in mice

TANG Fei1, ZHANG Yue-feng2, QU Feng-feng1, CHEN Yu-qiong1, YU Zhi1, NI De-jiang1   

  1. 1.Key Laboratory of Horticultural Plant Biology,Ministry of Education/College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;
    2.Wuhan Yellow Crane Tower Tea Co.,Ltd.,Wuhan 430032,China
  • Received:2018-08-16 Online:2019-06-25 Published:2019-12-05

摘要: 以昆明小鼠为试验对象,研究不同渥堆程度青砖茶对小鼠胃排空及小肠推进功能和肠道菌群的作用,并结合青砖茶理化成分对小鼠胃肠道功能各指标进行了相关性分析。理化检测表明,在晒青毛茶渥堆发酵过程中,游离氨基酸、茶多酚、可溶性糖、水浸出物、茶黄素、茶红素、EGC、EC、ECG、C、EGCG、酒石酸、苹果酸、乳酸含量随着渥堆发酵程度的增加而急剧下降,茶褐素、草酸、奎宁酸、琥珀酸、没食子酸含量上升。在连续给予小鼠青砖茶提取物15 d后发现,轻发酵、适度发酵青砖茶能有效促进胃排空和小肠蠕动作用,并能显著促进小鼠胃肠内双歧杆菌、乳杆菌生长繁殖,抑制肠杆菌、肠球菌数量,其中适度发酵青砖茶表现更优。结合不同渥堆程度青砖茶理化结果进行相关性分析,改善小鼠胃肠道功能的物质可能为茶褐素、草酸、奎宁酸、琥珀酸、柠檬酸、没食子酸。

关键词: 青砖茶, 渥堆, 胃排空, 小肠推进, 肠道菌群

Abstract: The aim of this study was to examine whether different fermentation levels of dark brick tea affected the gastric emptying, intestinal propulsion and intestinal microflora in Kunming mice by conducting correlation analysis between major chemical components of dark brick tea and gastrointestinal function of mice. Results indicated that during the fermentation process, the contents of free amino acids, polyphenols, soluble sugars, water extracts, theaflavins, thearubigins, epigallocatechin, epicatechin, catechin, epigallocatechin gallate, tartaric acid, malic acid and lactic acid were significantly decreased, while the contents of theabrowine, caffeine, oxalic acid, quininic acid, succinic acid as well as gallic acid were remarkably increased. After a consecutively 15 days intragastric administration of dark brick tea extract solution to mice, both dark brick tea fermented for 9 days and 15 days could significantly promote gastric emptying and intestinal propulsion, increase the amount of bifidobacterial and lactobacillus, as well as decrease the amount of enterobacteria and enterococcus, in which of the dark brick tea fermented for 15 days was better. Furthermore, the dark brick tea fermented for 15 days was superior to 9 days in terms of improving gastrointestinal function of mice. Correlation analysis showed the major functional components of dark brick tea might be theabrowine, oxalic acid, quininic acid, succinic acid, citric acid and gallic acid.

Key words: dark brick tea, fermentation, gastric emptying, intestinal propulsion, intestinal microflora

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