湖北农业科学 ›› 2019, Vol. 58 ›› Issue (15): 136-138.doi: 10.14088/j.cnki.issn0439-8114.2019.15.032

• 生物工程 • 上一篇    下一篇

漱口水对大蒜根尖细胞遗传损伤作用的研究

李俊辰, 刘忠湫, 蔡得阳, 黎金连, 钟海凤, 林军, 吴群英   

  1. 桂林医学院生物技术学院, 广西 桂林 541100
  • 收稿日期:2018-07-30 发布日期:2019-11-13
  • 通讯作者: 吴群英(1976-), 女, 广西桂林人, 副教授, 主要从事细胞生物学与遗传学的教学与科研工作, (电子信箱)qunyingwu@126.com。
  • 作者简介:李俊辰(1996-), 男(满族), 甘肃兰州人, 本科, 主要从事细胞生物学与遗传学的科研工作, (电话)15507737241(电子信箱)chenlijunlz@163.com;
  • 基金资助:
    广西2015年大学生创新创业训练计划项目(201510601034)

Effects of mouthwash on genetic damage of root tip cells of garlic

LI Jun-chen, LIU Zhong-qiu, CAI De-yang, LI Jin-lian, ZHONG Hai-feng, LIN Jun, WU Qun-ying   

  1. Guilin Medical University, College of Biotechnology, Guilin 541100, Guangxi, China
  • Received:2018-07-30 Published:2019-11-13

摘要: 用不同浓度的漱口水溶液分别处理大蒜根尖8、12和24 h, 利用微核技术, 研究漱口水对大蒜根尖细胞遗传损伤作用。结果表明, 与对照组相比, 漱口水处理组细胞微核率增加(P<0.05), 分裂细胞数减少, 分裂指数下降(P<0.05), 表明漱口水对大蒜根尖细胞有遗传损伤作用。

关键词: 漱口水, 微核率, 微核指数, 有丝分裂指数

Abstract: The research aimed to study the effect of mouthwash on the genetic damage of root tip cells of garlic, different concentrations of mouthwash were used separately to treat the root tip of garlic for 8, 12 and 24 h. The results showed that mouthwash induced the aberration of chromosomal and nuclear, the higher concentrations of mouthwash induced higher frequency of micronucleus rates(P<0.05). while he number of mitosis cell of divisions and mitotic index decreased(P<0.05), which were negatively correlated with the treatment time and dose of mouthwash. These results suggested mouthwash caused the genetic damage.

Key words: mouthwash, micronucleus rate, micronucleus index, mitotic index

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