湖北农业科学 ›› 2019, Vol. 58 ›› Issue (16): 107-109.doi: 10.14088/j.cnki.issn0439-8114.2019.16.024

• 畜牧·兽医 • 上一篇    下一篇

长顺绿壳蛋鸡FGF23基因遗传变异分析

吴磊1, 罗华伦1, 张依裕1, 覃媛钰1, 向程举2, 吴学树2   

  1. 1.贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵阳 550025;
    2.毕节市畜禽品种资源管理站,贵州 毕节 551700
  • 收稿日期:2018-12-20 出版日期:2019-08-25 发布日期:2019-11-12
  • 通讯作者: 张依裕(1976-),男,副教授,硕士生导师,博士,主要从事动物遗传资源保护与利用研究,(电子信箱)zyy8yyc@163.com。
  • 作者简介:吴 磊(1993-),男,贵州大方人,硕士,研究方向为畜牧养殖方向,(电话)15761636517(电子信箱)1475715385@qq.com
  • 基金资助:
    贵州省科技合作计划项目[黔科合LH字(2016)7454号]; 水西乌鸡选育与开发利用研究[毕科合字(2017)25号]

Analysis of genetic variation of FGF23 gene in Changshun green shell hen

WU Lei1, LUO Hua-lun1, ZHANG Yi-yu1, QIN Yuan-yu1, XIANG Cheng-ju2, WU Xue-shu2   

  1. 1.Key Laboratory of Plateau Mountain Animal Genetics,Breeding and Reproduction,Ministry of Education,College of Animal Science,Guizhou University,Guiyang 550025,China;
    2.Bijie City Livestock and Poultry Breeding Resource Management Station,Bijie 551700,Guizhou,China
  • Received:2018-12-20 Online:2019-08-25 Published:2019-11-12

摘要: 为进一步了解长顺绿壳蛋鸡FGF23基因的遗传效应,试验随机抽取同日出雏、健康无病、同等管理条件下饲喂10周龄的100只长顺绿壳蛋鸡,翅静脉采血,提取DNA,采用混合基因池进行PCR扩增,结合直接测序法对FGF23基因进行单核苷酸多态性(SNP)筛查。结果表明,在长顺绿壳蛋鸡FGF23基因启动子区共筛查到3个SNP突变位点(g.73424388,T>C;g.73424577,T<C;g.73424660,G>C),其中,g.73424388和g.73424660两个突变位点呈中度多态,g.73424577呈低度多态,说明长顺绿壳蛋鸡在自然群体中的遗传多样性较为丰富。

关键词: 长顺绿壳蛋鸡, FGF23基因, 单核苷酸多态性, 抗逆性

Abstract: To further understand the genetic effects of the FGF23 gene in Changshun green shell,100 random long-green hens fed with 10 weeks old were randomly selected from the same time, healthy and disease-free, under the same management conditions. Blood was collected from the wing veins, DNA was extracted, and PCR was performed using a mixed gene pool. Direct sequencing method was used to study the single nucleotide polymorphism(SNP) screening of FGF23 gene. Results showed that three SNP mutation sites were screened in the promoter region of FGF23 gene in Changshun green shell(g.73424388,T>C;g.73424577,T<C;g.73424660,G>C) Among them, g.73424388 and g.73424660 two mutation sites are moderate polypeptides, and g.73424577 is a low polypeptide. It showed that the genetic diversity of Changshun green shell hens is abundant in natural populations.

Key words: Changshun green shell hen, FGF23 gene, single nucleotide polymorphism, resistance

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