甘薯抗病虫相关基因SGT1的TRAP分析

doi:10.14088/j.cnki.issn0439-8114.2019.17.032

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (17): 119-122.doi: 10.14088/j.cnki.issn0439-8114.2019.17.032

甘薯抗病虫相关基因SGT1的TRAP分析

王崇^1,2, 杨新笋¹, 雷剑¹, 苏文瑾¹, 柴沙沙¹, 张文英², 王连军¹

1.湖北省农业科学院粮食作物研究所/湖北省甘薯工程技术研究中心/粮食作物种质创新与遗传改良湖北省重点实验室,武汉 430064;
2.长江大学农学院,湖北荆州 434025

收稿日期:2019-05-16 发布日期:2019-11-14
作者简介:王崇（1994-），男，湖北黄梅人，在读硕士研究生，研究方向为植物遗传育种研究，（电话）13986699677（电子信箱）wangchong199409@163.com；
基金资助:
国家重点研发计划项目（2018YFD1000700）; 国家现代甘薯产业技术体系建设项目（CARS-11-C-15）; 湖北省技术创新专项（重大项目）“甘薯抗小象甲新品种选育及综防栽培技术研究与示范”（2017ABA149）; 湖北省农业科学院特色学科项目; 湖北省农业科技创新中心资助项目（2007-620-001-03）; 湖北省技术创新专项（重大项目）“非洲和东南亚引进的主要农作物资源创新与应用”（2016ABA087）; 湖北省农业科学院青年拔尖人才培养计划项目

TRAP analysis of disease and pest resistance related gene SGT1 in sweetpotato

WANG Chong^1,2, YANG Xin-sun¹, LEI Jian¹, SU Wen-jin¹, CHAI Sha-sha¹, ZHANG Wen-ying², WANG Lian-jun¹

1.Institute of Food Corps, Hubei Academy of Agricultural Sciences/Hubei Engineering and Technology Research Center of Sweetpotato/Hubei Key Laboratory of Food Crops Germplasm and Genetic Improvement,Wuhan 430064,China;
2.College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China

Received:2019-05-16 Published:2019-11-14

摘要/Abstract

摘要： 以抗病虫相关基因SGT1作为靶标基因,设计出1条固定引物,与11条随机引物组合。利用这11对引物对试验材料鄂薯11和鄂紫薯13进行TRAP-PCR扩增,发现11对引物组合扩增出清晰条带,且表现出良好的多态性。研究获得与SGT1基因相关联的TRAP标记,该标记可进行甘薯[Ipomoea batatas（L.） Lam.]群体的遗传多样性分析,为甘薯的抗病虫育种提供理论依据。

关键词: 甘薯[Ipomoea batatas（L.） Lam.], 抗病虫, SGT1基因, TRAP标记

Abstract: Disease and pest resistance related gene SGT1 was used as a target gene to design a fixed primer,then for paring 11 pairs primers with 11 arbitrary primers. The 11 pairs of primers were used to amplify Eshu 11 and Ezishu 13 by TRAP-PCR. It was found that 11 pairs of primer combinations amplified clear bands and showed good polymorphism. In this study, TRAP markers associated with SGT1 gene were obtained, which can be used to analyze the genetic diversity of sweetpotato[Ipomoea batatas（L.） Lam.] population, and provide theoretical basis for disease and insect resistance breeding of sweetpotato.

Key words: sweetpotato[Ipomoea batatas（L.） Lam.], disease and insect resistance, SGT1 gene, TRAP marker

中图分类号:

Q789
S531

王崇, 杨新笋, 雷剑, 苏文瑾, 柴沙沙, 张文英, 王连军. 甘薯抗病虫相关基因SGT1的TRAP分析[J]. 湖北农业科学, 2019, 58(17): 119-122.

WANG Chong, YANG Xin-sun, LEI Jian, SU Wen-jin, CHAI Sha-sha, ZHANG Wen-ying, WANG Lian-jun. TRAP analysis of disease and pest resistance related gene SGT1 in sweetpotato[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(17): 119-122.

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甘薯抗病虫相关基因SGT1的TRAP分析

TRAP analysis of disease and pest resistance related gene SGT1 in sweetpotato

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