甘薯抗病相关基因TAO1-like的TRAP分析

doi:10.14088/j.cnki.issn0439-8114.2019.18.037

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (18): 152-155.doi: 10.14088/j.cnki.issn0439-8114.2019.18.037

甘薯抗病相关基因TAO1-like的TRAP分析

王崇^{1, 2}, 王连军², 雷剑², 苏文瑾², 柴沙沙², 杨新笋², 张文英¹

1.长江大学农学院,湖北荆州 434025;
2.湖北省农业科学院粮食作物研究所/湖北省甘薯工程技术研究中心/粮食作物种质创新与遗传改良湖北省重点实验室,武汉 430064

收稿日期:2018-11-29 出版日期:2019-09-25 发布日期:2019-11-08
通讯作者: 杨新笋（1967-）,男,研究员,主要研究方向为甘薯育种与栽培,（电子信箱）yangxins67@yahoo.com.cn;张文英（1972-）,男,教授,主要从事作物逆境抗性研究,（电子信箱）wyzhang@yangtzeu.edu.cn。
作者简介:王崇（1994-）,男,湖北黄梅人,硕士研究生,专业方向为植物遗传育种研究,（电子信箱）wangchong199409@163.com
基金资助:
国家现代甘薯产业技术体系建设项目（CARS-11-C-15）; 湖北省农业科学院特色学科项目; 湖北省农业科技创新中心资助项目（2007-620-001-03）; 湖北省技术创新专项（重大项目）（2017ABA149; 2016ABA087）; 湖北省农业科学院青年拔尖人才培养计划项目

TRAP analysis of disease resistance related gene TAO1-like in sweet potato

WANG Chong^{1, 2}, WANG Lian-jun², LEI Jian², SU Wen-jin², CHAI Sha-sha², YANG Xin-sun², ZHANG Wen-ying¹

1.College of Agriculture, Yangtze University,Jingzhou 434025,Hubei,China;
2.Hubei Academy of Agricultural Sciences,Institute of Food Corps/Hubei Sweet Potato Engineering and Technology Research Centre/Hubei Key Laboratory of Food Crops Germplasm and Genetic Improvement,Wuhan 430064,China

Received:2018-11-29 Online:2019-09-25 Published:2019-11-08

摘要/Abstract

摘要： NBS-LRR是植物中已分离抗病基因最大的一类,Target of AvrB Operation（TAO1）属于NBS-LRR类基因。以甘薯[Ipomoea batatas（L.） Lam.]抗病相关的TAO1基因作为靶标基因,设计出1条固定引物,与11条随机引物组合成11对引物。利用这11对引物对甘薯品种鄂薯11、鄂紫薯13进行TRAP-PCR扩增,发现11个引物组合扩增出清晰条带,且表现出良好的多态性。该研究获得与TAO1-like基因相关联的TRAP标记,该标记可进行甘薯群体的遗传多样性分析,为甘薯的抗病育种奠定基础。

关键词: 甘薯[Ipomoea batatas（L.） Lam.], 抗病, NBS-LRR类基因, TAO1, TRAP标记

Abstract: NBS-LRR is the largest class of isolated disease resistance genes in plants, Target of AvrB Operation（TAO1）belongs to NBS-LRR class genes. Using sweet potato disease resistance genes NBS-LRR as target gene to design one fixed primer,and for paring 11 pairs primers with 11 arbitrary primers. The 11 pairs of primers were used for TRAP-PCR amplification of sweet potato cultivars Eshu 11 and Ezishu 13. It was found that 11 primer combinations amplified clear bands and showed good polymorphism. The TRAP marker that associated with the TAO1-like gene were obtained, which could be used to analyze the genetic diversity of sweet potato population and provide a theoretical basis for disease resistance breeding of sweet potato.

Key words: sweet potato [Ipomoea batatas（L.） Lam.], disease resistance, NBS-LRR gene, TAO1, TRAP marker

中图分类号:

S531
Q78

王崇, 王连军, 雷剑, 苏文瑾, 柴沙沙, 杨新笋, 张文英. 甘薯抗病相关基因TAO1-like的TRAP分析[J]. 湖北农业科学, 2019, 58(18): 152-155.

WANG Chong, WANG Lian-jun, LEI Jian, SU Wen-jin, CHAI Sha-sha, YANG Xin-sun, ZHANG Wen-ying. TRAP analysis of disease resistance related gene TAO1-like in sweet potato[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(18): 152-155.

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甘薯抗病相关基因TAO1-like的TRAP分析

TRAP analysis of disease resistance related gene TAO1-like in sweet potato

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