关键词: 高效液相色谱-串联质谱, 果蔬, QuEchERs, 氨基甲酸酯类农药, 检测

Abstract: A high performance liquid chromatography-tandem mass spectrometric method with modified QuEchERs method for sample preparation was developed for the simultaneous determination of 12 carbamate pesticides in vegetables and fruits. The samples were extracted with acetonitrile,purified with QuEchERs method and filtrated by 0.22 μm microporous filters. The separation was carried on an ZOBRAX EclipseXDB-C₁₈ Colum（150 mm×4.6 mm,5 μm） by a gradient elution using methanol -5 mmol/L ammonium acetate solution（contain 0.1% formic acid aqueous）as mobile phase. The analytes were detected by tandem mass spectrometry under multiple reaction monitoring（MRM） mode with positive electrospray ionization（ESI+） and quantified by external standard calibration method. The correlation coefficients of the standard calibration curves for the 12 analytes were all above 0.996 6. The limits of detection was 0.1 μg/L. The blank samples were fortified at three leves,and the average recoveries ranged from 80.0% to 127.0% with the RSDs from 1.1% to 10.2%. The method developed was easy to operate,sensitive,and had good purification effect. It could be applied for the rapid determination of 12 carbamate pesticides in vegetables and fruits.

Key words: high performance liquid chromatography-tandem mass spectrometry, vegetables and fruits, QuEchERs, carbamate pesticides, detection