湖北农业科学 ›› 2019, Vol. 58 ›› Issue (21): 173-175.doi: 10.14088/j.cnki.issn0439-8114.2019.21.038

• 检测分析 • 上一篇    下一篇

东北林蛙和牛蛙腿部肌肉的基本营养成分分析

田新民, 石兰英   

  1. 牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江 157011
  • 收稿日期:2019-03-12 出版日期:2019-11-10 发布日期:2019-11-27
  • 作者简介:田新民(1982-),男,黑龙江明水人,副教授,博士,主要从事动物生态学研究,(电话)15845303654(电子信箱)swxtxm@126.com。
  • 基金资助:
    牡丹江师范学院科研项目(GP2019005; QN2019009); 黑龙江省基本科研业务费支持项目(1353ZD006); 牡丹江师范学院优秀青年骨干教师培养计划项目(MQP201405)

Analysis of basic nutritional components of leg muscle of Rana dybowskii and Lithobates catesbeiana

TIAN Xin-min, SHI Lan-ying   

  1. College of Life Sciences and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China
  • Received:2019-03-12 Online:2019-11-10 Published:2019-11-27

摘要: 为了分析东北林蛙和牛蛙腿部肌肉的基本营养成分,试验分别采用105 ℃恒质法、考马斯亮蓝染色法、凯氏定氮法、索氏提取法、3,5-二硝基水杨酸比色法和马福炉灰化法对东北林蛙和牛蛙腿部肌肉的水分、水溶性蛋白、盐溶性蛋白、总蛋白、粗脂肪、还原糖、总糖和灰分含量进行了比较分析。结果表明,东北林蛙和牛蛙的腿部肌肉水分含量分别为79.21%±0.40%和76.87%±0.76%,水溶性蛋白为2.91%±0.37%和2.56%±0.07%、盐溶性蛋白为6.07%±0.52%和6.44%±0.48%、总蛋白为24.57%±1.86%和25.08%±2.20%、粗脂肪为0.50%±0.05%和0.59%±0.10%、还原糖为0.15%±0.01%和0.33%±0.02%、总糖为2.41%±0.13%和2.34%±0.04%、灰分为4.41%±0.17%和6.63%±0.42%。其中水分、还原糖和灰分含量差异显著(P<0.05),其他成分差异不显著(P>0.05)。

关键词: 东北林蛙, 牛蛙, 肌肉, 基本营养成分

Abstract: In order to analyze the basic nutritional components of the muscle of Rana dybowskii and Lithobates catesbeiana, and provide reference for food development. The water content, water soluble protein, salt soluble protein, total protein, crude fat, reducing sugar, total sugar and the ash content of leg muscles of Rana dybowskii and Lithobates catesbeiana were determined by 105 ℃ drying constant mass method, coomassie brilliant blue dyeing method, kjeldahl method, soxhlet extraction method, 3,5-dinitrosalicylic acid colorimetric method and volatilization constant mass method. The contents were compared, the results show that the moisture content of leg muscles of Rana dybowskii and Lithobates catesbeiana were 79.21%±0.40% and 76.87%±0.76% respectively, water-soluble protein was 2.91%±0.37% and 2.56%±0.07%, salt-soluble protein was 6.07%±0.52% and 6.44%±0.48%, total protein was 24.57%±1.86% and 25.08%±2.20%, crude fat was 0.50%±0.05% and 0.59%±0.10%, reducing sugar was 0.15%±0.01% and 0.33%±0.02. The total sugar content was 2.41%±0.13% and 2.34%±0.04% respectively. The ash content was 4.41%±0.17% and 6.63%±0.42%. Among them, the contents of water, reducing sugar and ash were significantly different between Rana dybowskii and Lithobates catesbeianaP<0.05), but there was no difference in other components (P>0.05).

Key words: Rana dybowskii, Lithobates catesbeiana, muscle, basic nutritional components

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