湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 215-217.doi: 10.14088/j.cnki.issn0439-8114.2019.23.053

• 生物工程 • 上一篇    下一篇

佛手瓜染色体核型分析

杨婷1, 王亚1, 张素勤1, 陈星灼1, 耿广东1, 陈丽2   

  1. 1.贵州大学农学院,贵阳 550025;
    2.普定县农业示范园区,贵州 安顺 562100
  • 收稿日期:2019-01-17 出版日期:2019-12-10 发布日期:2019-12-18
  • 通讯作者: 耿广东,男,教授,主要从事蔬菜栽培生理与生物技术方面的教研工作,(电子信箱)genggd213@163.com。
  • 作者简介:杨 婷(1991-),女,贵州德江人,在读硕士研究生,研究方向为遗传育种,(电话)18785171705(电子信箱)yangting_122@163.com;共同第一作者,王 亚(1992-),男,河南息县人,在读硕士研究生,研究方向为遗传育种,(电话)17337529132(电子信箱)530026291@qq.com
  • 基金资助:
    贵州省科技厅重大专项计划项目(黔科合重大专项字[2016]3002号); 贵州省农业攻关项目(黔科合支撑[2016]2603号; 黔科合支撑[2018]2330号)

Chromosomal karyotype analysis of Sechium edule Swartz

YANG Ting1, WANG Ya1, ZHANG Su-qin1, CHEN Xing-zhuo1, GENG Guang-dong1, CHEN Li2   

  1. 1.College of Agriculture,Guizhou University,Guiyang 550025,China;
    2.Agricultural Park of Puding,Anshun 562100,Guizhou,China
  • Received:2019-01-17 Online:2019-12-10 Published:2019-12-18

摘要: 采用滴片技术获得佛手瓜(Sechium edule Swartz)根尖细胞染色体片子,并进行染色体核型分析,以探索佛手瓜染色体核型特点,为佛手瓜及相近植物遗传育种提供细胞学依据。结果显示,佛手瓜具有14对染色体,均为中部着丝粒染色体(m),每条染色体上均未发现随体,其核型公式为2n=2x=28=28m;核型不对称系数为53.55%,属于对称核型;佛手瓜的核型为1A,是一种进化程度低的葫芦科蔬菜作物。

关键词: 佛手瓜(Sechium edule Swartz), 染色体, 核型

Abstract: To provide cytological basis for genetics and breeding for Sechium edule Swartz and its similar plants, chromosome preparations from root tip cell of Sechium edule Swartz were obtained by using dropping slide technique, and its karyotype was analyzed in order to understand chromosomal karyotype characteristics. The result showed that the genome of Sechium edule Swartz possessed 14 pairs of chromosomes, all of which were metacentric chromosomes(m), and no any satellite was found in them. Its karyotype formula was 2n=2x=28=28m. The asymmetrical karyotype coefficient was 53.55%, which was symmetrical karyotype. Its karyotype belonged to the type 1A. Therefore, the evolution degree of Sechium edule Swartz was low among Cucurbitace.

Key words: Sechium edule Swartz, chromosome, karyotype

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