湖北农业科学 ›› 2020, Vol. 59 ›› Issue (14): 69-75.doi: 10.14088/j.cnki.issn0439-8114.2020.14.012

• 资源·环境 • 上一篇    下一篇

基于园林废弃物真菌菌剂的制备及应用

彭春燕1, 高育慧1, 吴华南2, 郑卫国1, 刘天翔1, 李军娟1, 喻东1, 郑雯芳1   

  1. 1.深圳文科园林股份有限公司,广东 深圳 518000;
    2.北京大学深圳研究生院,广东 深圳 518000
  • 收稿日期:2019-12-16 出版日期:2020-07-25 发布日期:2020-09-14
  • 通讯作者: 刘天翔(1987-),男,河南南阳人,工程师,硕士,主要从事环境微生物技术研究,(电子信箱)liutianxiang@wkyy.com。
  • 作者简介:彭春燕(1991-),女,江西赣州人,工程师,硕士,主要从事环境微生物技术研究,(电话)18813674860(电子信箱)1197043558@qq.com
  • 基金资助:
    深圳市战略新兴产业发展专项资金项目(深发改[2017]1447号)

Preparation of fungus based on garden waste and its application

PENG Chun-yan1, GAO Yu-hui1, WU Hua-nan2, ZHENG Wei-guo1, LIU Tian-xiang1, LI Jun-juan1, YU Dong1, ZHENG Wen-fang1   

  1. 1. Shenzhen Wenke Landscape Co., Ltd., Shenzhen 518000,Guangdong,China;
    2. Peking University Shenzhen Graduate School, Shenzhen 518000,Guangdong,China
  • Received:2019-12-16 Online:2020-07-25 Published:2020-09-14

摘要: 采用“以废治污”的理念,将以园林废弃物为基质制备的真菌菌剂应用于污染河水中,以实现固体废弃物与废水的联动处理。考察园林废弃物的含量、基质厚度和初始含水率对该菌株孢子制备的影响,并用响应面法优化制备工艺。结果表明,影响孢子产量的因子主次顺序为基质厚度、园林废弃物含量和含水率;最佳制备工艺条件为园林废弃物含量46%、基质厚度3 cm、初始含水率57%,得到的孢子数为4.75×109 CFU/g,该结果与回归方程预测值接近;将制备的孢子活化,并以0.50‰的接种量投加至污染河水中,曝气处理54 h后,COD、NH3-N和TP的去除率分别达84.97%、97.07%和51.64%;连续试验表明,该菌剂能够有效地降低河水中的COD、NH3-N和TP等污染物,且具有较高的稳定性。

关键词: 园林废弃物, 真菌, 废水, 制备, 响应面法, 曝气

Abstract: Based on the concept of “using waste to control pollution”, a fungus with high pollution resistance prepared from garden waste was applied to polluted river water to realize the linkage treatment of solid waste and waste water. Garden waste content, substrate thickness and initial moisture content were taken as influencing factors. The effect of the content, substrate thickness and initial water content of garden waste on the preparation of spores was investigated, and the preparation process was optimized by response surface method. The results showed that the most influential factor on the yield of spore was substrate thickness, followed by garden waste content and initial moisture content. The optimum conditions were garden waste content 46%, substrate thickness 3 cm and initial moisture content 57%. Under such conditions, the actual results were close to the predicted values of regression equation. Then the prepared spores were activated and added to polluted river water at an inoculum of 0.50‰. After 54 hours of aeration, the removal rates of COD, NH3-N and TP reached 84.97%, 97.07% and 51.64%, respectively. Continuous experiments demonstrated that this integrated system could effectively remove pollutants such as COD, NH3-N and TP in polluted river water with high stability.

Key words: garden waste, fungus, waste water, preparation, response surface methodology, aeration

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