HPLC法分析叶用芥菜种子中莱菔硫烷含量

doi:10.14088/j.cnki.issn0439-8114.2020.17.035

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (17): 154-157.doi: 10.14088/j.cnki.issn0439-8114.2020.17.035

HPLC法分析叶用芥菜种子中莱菔硫烷含量

兰梅, 杨红丽, 胡靖锋, 张丽琴, 徐学忠, 和江明

云南省农业科学院园艺作物研究所/国家蔬菜改良中心云南分中心,昆明 650205

收稿日期:2019-09-16 发布日期:2020-10-23
通讯作者: 和江明（1969-）,男（纳西族）,云南香格里拉人,研究员,主要从事十字花科作物育种研究,（电话） 13238695391（电子信箱）hejiangming666@qq.com。
作者简介:兰梅（1986-）,女,云南昆明人,助理研究员,硕士,主要从事十字花科蔬菜生物技术研究,（电话）18288656601（电子信箱）564325868@qq.com。
基金资助:
云南省科技厅应用基础研究青年基金项目（2017FD200）; 大宗蔬菜产业技术体系昆明综合试验站项目（CARS-23-G37）; 云南省蔬菜现代农业产业技术体系项目（2017KJTX0011）; 云南主要外销蔬菜绿色关键技术研究和集成示范项目（2019ZG001）

HPLC analysis of sulforaphane in different kinds of leaf mustard （Brassica juncea Coss. var. foliosa Bailey） seeds

LAN Mei, YANG Hong-li, HU Jing-feng, ZHANG Li-qin, XU Xue-zhong, HE Jiang-ming

Horticultural Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Branch of National Vegetable Improvement Center,Kunming 650205,China

Received:2019-09-16 Published:2020-10-23

摘要/Abstract

摘要： 为了筛选富含莱菔硫烷的叶用芥菜材料,以20份叶用芥菜种子为试材,采用高效液相色谱法（HPLC）测定芥菜种子中的莱菔硫烷含量,分析不同品种之间的莱菔硫烷含量变化规律。结果表明,20份叶用芥菜材料种子中莱菔硫烷含量为11.79~226.72 mg/kg（DW）,供试材料间莱菔硫烷含量差异达显著水平,并获得1份莱菔硫烷含量较高的材料（太乐四季青）,含量为226.72 mg/kg（DW）;1份莱菔硫烷含量最低的材料（圆叶春菜）,含量为11.79 mg/kg（DW）,最高含量约是最低含量的19.2倍,是研究莱菔硫烷含量变化规律的优良材料,也为下一步深入开展莱菔硫烷代谢机理研究奠定了基础。

关键词: 叶用芥菜, 莱菔硫烷, 高效液相色谱（HPLC）

Abstract: In order to get the resources of leaf mustard with abundant sulforaphane,seeds of twenty cultivars were used to analyze and compare the contents of sulforaphaneby high performance liquid chromatography （HPLC）. The results showed that the range ofvariatoin of sulforaphane was 11.79~226.72 mg/kg（DW） with significant differences among tested lines. The material （Tai le sijiqing） with highest content of sulforaphane,which is 226.72 mg/kg （DW）,was about 19.2 times compared with the lowest one （Spring cobbage with round leaves） which is 11.79 mg/kg （DW）. It provides an foundational material for further research on rule of variation and the mechanism of metabolism of sulforaphan.

Key words: leaf mustard, sulforaphane, high performance liquid chromatography （HPLC）

中图分类号:

S637.2

兰梅, 杨红丽, 胡靖锋, 张丽琴, 徐学忠, 和江明. HPLC法分析叶用芥菜种子中莱菔硫烷含量[J]. 湖北农业科学, 2020, 59(17): 154-157.

LAN Mei, YANG Hong-li, HU Jing-feng, ZHANG Li-qin, XU Xue-zhong, HE Jiang-ming. HPLC analysis of sulforaphane in different kinds of leaf mustard （Brassica juncea Coss. var. foliosa Bailey） seeds[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 154-157.

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HPLC法分析叶用芥菜种子中莱菔硫烷含量

HPLC analysis of sulforaphane in different kinds of leaf mustard （Brassica juncea Coss. var. foliosa Bailey） seeds

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