湖北贝母石油醚段的抗菌活性和化学成分研究

doi:10.14088/j.cnki.issn0439-8114.2020.22.033

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (22): 160-165.doi: 10.14088/j.cnki.issn0439-8114.2020.22.033

湖北贝母石油醚段的抗菌活性和化学成分研究

方诗琪^1,2, 李宇¹, 黄东海^1,2, 艾伦强^1,3, 刘翠君^1,2,3, 何美军^1,3

1.湖北省农业科学院中药材研究所,湖北恩施 445000;
2.国家中药材产业技术体系恩施综合试验站,湖北恩施 445000;
3.湖北省农业科技创新中心药材分中心,湖北恩施 445000

收稿日期:2020-07-15 出版日期:2020-11-25 发布日期:2020-12-30
通讯作者: 何美军(1974-),男(土家族),湖北恩施人,副研究员,博士,主要从事中药材天然产物研究,(电子信箱)840940513@qq.com
作者简介:方诗琪(1994-),女,重庆万州人,助理研究员,硕士,主要从事中药材天然产物研究,(电话)15974834611(电子信箱)171725944@qq.com
基金资助:
湖北省农业科学院中药材研究所青年基金项目(2019ZCYJJ02)

Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia

FANG Shi-qi^1,2, LI Yu¹, HUANG Dong-hai^1,2, AI Lun-qiang^1,3, LIU Cui-jun^1,2,3, HE Mei-jun^1,3

1. Institute of Chinese Herbel Medicine,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China;
2. National Herbal Medicine Industry Technology System,Enshi Comprehensive Test Station,Enshi 445000,Hubei,China;
3. Chinese Herbal Medicine Subcenter of Hubei Agricultural Science and Technology Innovation Centre,Enshi 445000,Hubei,China

Received:2020-07-15 Online:2020-11-25 Published:2020-12-30

摘要/Abstract

摘要： 为探究湖北贝母(Fritillaria hupehensis Hsiao et k.c.Hsia)石油醚段的抗菌活性和化学成分,将湖北贝母经60%乙醇溶液超声提取获得总浸膏,再采用石油醚萃取获得石油醚段浸膏,利用滤纸片法、稀释倒平板法、超微量紫外分光光度计以及电导仪对湖北贝母石油醚段的抗菌活性和机制进行了探索;同时,利用GC-MS、1D和2D NMR等对湖北贝母石油醚段的成分进行了探索。结果表明,湖北贝母石油醚段溶液对于苏云金芽孢杆菌、绿脓杆菌有中度敏感抑制活性,对肺炎克雷伯杆菌ATCC13883、金黄色葡萄球菌ATCC29213、枯草芽孢杆菌有轻度敏感抑制活性。其中,只有苏云金芽孢杆菌、枯草芽孢杆菌、绿脓杆菌的MIC/MBC小于4.0 mg/mL。湖北贝母石油醚段很可能通过破坏细胞膜从而减缓了苏云金芽孢杆菌、枯草芽孢杆菌、绿脓杆菌生长,从而达到抗菌的效果,并获得了对应总离子流中的27种化合物。

关键词: 湖北贝母(Fritillaria hupehensis Hsiao et k.c.Hsia), 抗菌活性, 石油醚段, 化合物

Abstract: Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia,Fritillaria hupehensis Hsiao et k.c.Hsia were collected from Enshi area,Hubei province,China,whoes total extract was obtained by ultrasonic extraction with ethanol solution (60%).And the total extract to obtain petroleum ether segment were extracted.The antibacterial activity and mechanism of petroleum ether segment of Fritillaria hupehensis were studied by means of filter paper method,dilution pour plate method,ultra micro ultraviolet spectrophotometer and conductivity meter.The composition of petroleum ether segment from Fritillaria hupehensis was studied by means of GC-MS,1D and2D NMR.Results showed that Hubei Fritillaria petroleum ether solution was moderately inhibitory sensitive on Bacillus thuringiensis and Pseudomonas aeruginosa,and mildly inhibitory sensitive on Klebsiella pneumonia ATCC13883,Staphylococcus aureus ATCC29213,and Bacillus subtilis.Among them,the MIC/MBC of Bacillus thuringiensis,Bacillus subtilis,and Pseudomonas aeruginosa were less than4.0 mg/mL.Through the exploration of antibacterial mechanism,the petroleum ether section of Fritillaria fragrans Hubei probably slowed the growth of Bacillus thuringiensis,Bacillus subtilis,Pseudomonas aeruginosa by destroying the cell membrane,and finally achieved antibacterial effect.This study also explored the composition of the petroleum ether Extracts from Fritillaria hupehensis Hsiao et k.c.Hsia.Total ion chromatograms of the petroleum ether Extracts from Fritillaria hupehensis Hsiao et k.c.Hsia were obtained and compared with the NIST08.LIB standard library based on retention time and mass spectrometry data.Finally27 compounds of the total ion chromatogram were obtained.

Key words: Fritillaria hupehensis Hsiao et k.c.Hsia, antimicrobial activity, petroleum ether extract, compound

中图分类号:

方诗琪, 李宇, 黄东海, 艾伦强, 刘翠君, 何美军. 湖北贝母石油醚段的抗菌活性和化学成分研究[J]. 湖北农业科学, 2020, 59(22): 160-165.

FANG Shi-qi, LI Yu, HUANG Dong-hai, AI Lun-qiang, LIU Cui-jun, HE Mei-jun. Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 160-165.

参考文献

[1] 刘杰书.湖北贝母的本草考证及其品质评价[J].湖北中医杂志,2001,23(7):50-51.
[2] 苏敏,尚志钧.新修本草[M].第2版.合肥:安徽科学技术出版社,2004.
[3] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.286-287.
[4] 陈心启.中国植物志.第14卷[M].北京:科学出版社,1980.111.
[5] 杨志刚,熊德武.湖北贝母的有性繁殖技术[J].科技创业月刊,2000(1):30-31.
[6] TACCONELLI E,CARRARA E,SAVOLDI A,et al.Discovery,research,and development of new antibiotics:The WHO priority list of antibiotic-resistant bacteria and tuberculosis[J].The lancet infectious diseases,2018,18(3):318-327.
[7] 张铁焕,吴玉霞,奠佐红,等.19 种常见中药材醇提物的体外抗菌活性筛选[J].广西植物,DOI:10.11931/guihaia.gxzw201903004.
[8] PARVATHY K S,NEGI P S,SRINIVAS P.Antioxidant,antimutagenic and antibacterial activities of curcumin-β-diglucoside[J].Food chemistry,2012,115(1):265-271.
[9] LIU F,WANG F,DU L,et al.Antibacterial and antibiofilm activity of phenyllactic acid against Enterobacter cloacae[J].Food control,2018,84:442-448.
[10] DIAO W R,HU Q P,ZHANG H,et al.Chemical composition,antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.)[J].Food control,2014,35(1):109-116.
[11] LV F,LIANG H,YUAN Q,et al.In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms[J].Food research international,2011,44(9):3057-3064.
[12] CHÉRIGO L,PEREDA-MIRANDA R,GIBBONS S.Bacterial resistance modifying tetrasaccharide agents from Ipomoea murucoides[J].Phytochemistry,2009,70(2):222-227.
[13] OKUSA P N,PENGE O,DEVLEESCHOUWER M,et al.Direct and indirect antimicrobial effects and antioxidant activity of Cordia gilletii De Wild (Boraginaceae)[J].Journal of ethnopharmacology,2007,112(3):476-481.
[14] 钱丽红.茶多酚对金黄色葡萄球菌和铜绿假单胞菌的抑菌机理[J].微生物学通报,2010,37(11):1628-1633.
[15] 郭丽丽,王小敏,秦楠,等.黄芪茎叶总皂苷提取物的抑菌活性研究[J].现代食品科技,2019,35(1):88-94.

湖北贝母石油醚段的抗菌活性和化学成分研究

Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia

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