关键词: 芹菜（Apium graveolens L.）, 酚类化合物, 高效液相色谱法, 聚类分析

Abstract: Taking 10 types of celery （Apium graveolens L.） as test materials, a Venusil XBP Cl8 （L） chromatographic column （250 mm×4.6 mm, 5 μm） was used. Using 0.2% acetic acid solution-methanol as the mobile phase, gradient elution was performed at a flow rate of 0.5 mL/min; a PDA detector with a detection wavelength of 240 nm was adopted. A method was established for the simultaneous determination of 11 phenolic compounds in celery using high-performance liquid chromatography （HPLC）, and the selection of chromatographic columns, mobile phase conditions, and gradient elution conditions were optimized. The results showed that the linear relationship of 11 phenolic substances was good ranging from 0.5 to 10.0 μg/mL,and the correlation coefficient was greater than or equal to 0.990. Recovery experiments were conducted on celery samples with spiked levels of 0.2, 0.4 and 1.0 mg/kg, with recoveries ranging from 71.90% to 134.00% and relative standard deviation （RSD） ranging from 0.30% to 4.00%. The detection limit （LOD） and quantification limit （LOQ） were 0.07~0.29 mg/kg and 0.20~0.84 mg/kg, respectively. All active ingredients had good stability within 24 hours, and the relative standard deviation was less than 3.00%. This method had been successfully applied to the determination of 11 phenolic compounds in 10 types of celery. Combined with cluster analysis, it was found that there were similarities in phenolic components among different varieties of celery, but there were differences in their contents among different varieties. This method had high sensitivity and precision, and could provide technical support for the analysis of 11 phenolic compounds in celery.

Key words: celery（Apium graveolens L.）, phenolic compounds, high-performance liquid chromatography, cluster analysis