中华管鞭虾中虾青素的肠吸收特性研究

doi:10.14088/j.cnki.issn0439-8114.2021.20.027

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (20): 134-138.doi: 10.14088/j.cnki.issn0439-8114.2021.20.027

中华管鞭虾中虾青素的肠吸收特性研究

贾喆, 许艳, 刘欣妍, 张肖瑕, 宋茹

浙江海洋大学食品与药学学院,浙江舟山 316022

收稿日期:2021-05-10 出版日期:2021-10-25 发布日期:2021-11-05
通讯作者: 宋茹（1976-）,女,辽宁海城人,教授,博士生导师,博士,主要从事水产品加工与贮藏、食品化学与营养支持研究,（电话）13505800634（电子信箱）rusong656@163.com。
作者简介:贾喆（1996-）,男,河北保定人,在读硕士研究生,研究方向为食品加工与安全,（电话）17858801053（电子信箱）jzjzjjj@126.com。
基金资助:
国家重点研发计划项目（2018YFD0901105）; 浙江海洋大学优秀硕士论文培育项目（2019-41）

Study on intestinal absorption characteristics of astaxanthin extracted from Solenocera crassicornis

JIA Zhe, XU Yan, LIU Xin-yan, ZHANG Xiao-xia, SONG Ru

School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China

Received:2021-05-10 Online:2021-10-25 Published:2021-11-05

摘要/Abstract

摘要： 对Sprague Dawley雄性大鼠进行单次大剂量灌胃皂化和未皂化虾青素,采用高效液相色谱法分析灌胃6 h和12 h后小肠不同部位内容物中虾青素含量,并检测连续灌胃14 d后大鼠新鲜粪便中虾青素总量、游离型和酯型虾青素含量。结果表明,灌胃6 h后,大鼠空肠、回肠和结肠内容物中均未检测到虾青素;灌胃12 h后,皂化和未皂化虾青素组在结肠内容物中均检测出虾青素,且皂化组虾青素总量（283.221±89.50） μg/g显著高于未皂化组（126.850±40.49） μg/g;连续灌胃14 d后,大鼠粪便中皂化组虾青素的残留量显著低于未皂化组,且主要为游离型虾青素（11.12±0.36） μg/g,而未皂化虾青素组大鼠粪便中游离型和酯型虾青素含量分别为（21.78±3.40） μg/g和（7.69±0.32） μg/g。因此,皂化后中华管鞭虾的虾青素提取物较未皂化的虾青素提取物有更好的肠吸收性。

关键词: 中华管鞭虾, 虾青素, 肠吸收, 游离型虾青素, 酯型虾青素

Abstract: The Sprague Dawley male rats were given a single high-dose gavage with saponified and unsaponified astaxanthin, the content of astaxanthin in different parts of the small intestine after 6 h and 12 h were determined, and the total content of astaxanthin, free astaxanthin and ester astaxanthin in fresh feces of rats after 14 d of continuous gavage were detected by HPLC. The results showed that after 6 h of gavage,astaxanthin was not detected in the jejunum, ileum and colon of rats. After 12 h of gavage, astaxanthin was detected in the colon contents of both the saponified and unsaponified astaxanthin group, and the content of astaxanthin in the saponified group （283.221±89.50 μg/g） was significantly higher than that in the unsaponified group （126.850±40.49 μg/g）. After 14 d of continuous gavage, the astaxanthin residue from in feces of rats in the saponified group was significantly lower than that in the unsaponified group, and the astaxanthin residue was mainly composed of free astaxanthin（11.12±0.36 μg/g）. However, the contents of free astaxanthin and ester astaxanthin in the rats feces of unsaponified group were 21.78±3.40 μg/g and 7.69±0.32 μg/g, respectively. Therefore, the saponified astaxanthin derived from Solenocera crassicornis showed better intestinal absorption compared with the unsaponified astaxanthin.

Key words: Solenocera crassicornis, astaxanthin, intestinal absorption, free astaxanthin, ester astaxanthin

中图分类号:

TS254.9

贾喆, 许艳, 刘欣妍, 张肖瑕, 宋茹. 中华管鞭虾中虾青素的肠吸收特性研究[J]. 湖北农业科学, 2021, 60(20): 134-138.

JIA Zhe, XU Yan, LIU Xin-yan, ZHANG Xiao-xia, SONG Ru. Study on intestinal absorption characteristics of astaxanthin extracted from Solenocera crassicornis[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(20): 134-138.

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中华管鞭虾中虾青素的肠吸收特性研究

Study on intestinal absorption characteristics of astaxanthin extracted from Solenocera crassicornis

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