虾青素法夫酵母β-胡萝卜素转化酶的生物信息学分析

doi:10.14088/j.cnki.issn0439-8114.2020.01.036

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (1): 156-158.doi: 10.14088/j.cnki.issn0439-8114.2020.01.036

虾青素法夫酵母β-胡萝卜素转化酶的生物信息学分析

张爽, 许勇, 童琳, 张忠东, 叶红

安徽省医学科学研究院,合肥 230061

收稿日期:2019-04-04 出版日期:2020-01-10 发布日期:2020-01-10
通讯作者: 叶红（1973-）,女,安徽颍上人,副研究员,博士,主要从事微生物与预防医学研究工作,（电子信箱）yehong2000312@126.com。
作者简介:张爽（1988-）,女,江苏南京人,助理研究员,硕士,主要从事药学与预防医学研究工作,（电话）13855144568（电子信箱）359978812@qq.com
基金资助:
安徽省卫计委科研课题（2018YK002）

Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma

ZHANG Shuang, XU Yong, TONG Lin, ZHANG Zhong-dong, YE Hong

Anhui Academy of Medical Sciences,Hefei 230061,China

Received:2019-04-04 Online:2020-01-10 Published:2020-01-10

摘要/Abstract

摘要： 以虾青素中法夫酵母酶氨基酸序列为研究对象,利用ProtParam、SignalP、PSORT、ProtScale、SOMPA、PredictProtein、PROSITE、COILS和SWISS-MODEL等在线预测工具,对其理化性质、亚细胞定位、亲疏水性、二级结构、三级结构等进行分析。结果表明,该转化酶由557个氨基酸残基构成,是一种无信号肽,具有跨膜结构,定位于细胞色素氧化酶中的可溶性亲水蛋白。α螺旋和无规卷曲为主要的二级结构元件。三级结构以PDB ID 5vcc.1的B链为模板,功能预测为细胞色素P450。生物信息学手段分析预测结果可靠性较高,为进一步研究虾青素的生物合成调控机制提供了理论依据。

关键词: 虾青素, 法夫酵母, β-胡萝卜素转化酶, 生物信息学

Abstract: The amino acid sequence of the yeast extract of Astaxanthin was used as the research object. The physical and chemical properties and subcellular localization were determined by online prediction tools such as ProtParam, SignalP, PSORT, ProtScale, SOMPA, PredictProtein, PROSITE, COILS and SWISS-MODEL., pro-hydrophobicity, secondary structure, tertiary structure, etc. were analyzed. The invertase consisted of 557 amino acid residues and was a signal-free peptide, a transmembrane structure, and a soluble hydrophilic protein localized in cytochrome oxidase. The alpha helix and random coil are the primary secondary structural elements. The tertiary structure uses the B-chain of PDB ID 5vcc.1 as a template, and its function is predicted to be cytochrome P450. The bioinformatics method is more reliable in analyzing and predicting the results, which lays a theoretical foundation for further research on the biosynthesis regulation mechanism of astaxanthin.

Key words: astaxanthin, phaffia rhodozyma, β-carotene converting enzyme, bioinformatics

中图分类号:

S963.73

张爽, 许勇, 童琳, 张忠东, 叶红. 虾青素法夫酵母β-胡萝卜素转化酶的生物信息学分析[J]. 湖北农业科学, 2020, 59(1): 156-158.

ZHANG Shuang, XU Yong, TONG Lin, ZHANG Zhong-dong, YE Hong. Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 156-158.

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虾青素法夫酵母β-胡萝卜素转化酶的生物信息学分析

Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma

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