Alternaria perpunctulata NBERC_H56菌株对马唐的除草活性及其次级代谢产物的分离鉴定

doi:10.14088/j.cnki.issn0439-8114.2021.24.023

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (24): 98-100.doi: 10.14088/j.cnki.issn0439-8114.2021.24.023

Alternaria perpunctulata NBERC_H56菌株对马唐的除草活性及其次级代谢产物的分离鉴定

吴兆圆¹, 任梦瑶², 张志刚¹, 刘芳¹, 张亚妮¹, 方伟¹

1.湖北省生物农药工程研究中心,武汉 430064;
2.武汉科技大学医学院,武汉 430081

收稿日期:2020-11-15 出版日期:2021-12-25 发布日期:2022-01-04
通讯作者: 方伟,男,副研究员,主要从事微生物农药资源筛选方面的工作,（电子信箱）wei.fang@nberc.com。
作者简介:吴兆圆（1985-）,女,湖北石首人,副研究员,博士,主要从事农药活性微生物资源研究,（电话）15102708891（电子信箱）zhaoyuan.wu@nberc.com;
基金资助:
湖北省农业科技创新中心项目（2019-620-000-001-27）

Herbicidal activity of Alternaria perpunctulata NBERC_H56 to Digitaria sanguinalis and the secondary metabolites produced by the strain

WU Zhao-yuan¹, REN Meng-yao², ZHANG Zhi-gang¹, LIU Fang¹, ZHANG Ya-ni¹, FANG Wei¹

1. Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China;
2. College of Medical,Wuhan University of Science and Technology,Wuhan 430081,China

Received:2020-11-15 Online:2021-12-25 Published:2022-01-04

摘要/Abstract

摘要： 从罹病的马唐（Digitaria sanguinalis）植株上分离得到1株病原真菌NBERC_H56,鉴定为Alternaria perpunctulata,其发酵液对马唐幼苗的生长具有明显的抑制作用。为明确菌株NBERC_H56的活性物质基础,对其次级代谢产物进行研究,通过菌株大量发酵,经提取和制备高效液相色谱分离纯化得到3个化合物,经LC-MS以及NMR鉴定为Curvulin（1）、Alternariol（2）和Zinniol（3）。研究首次发现真菌Alternaria perpunctulata具有除草活性,并首次对该菌产生的次级代谢产物进行了分离鉴定。

关键词: 马唐（Digitaria sanguinalis）, 链格孢菌, 除草活性, 次级代谢产物

Abstract: A pathogenic fungus NBERC_H56 was isolated from the diseased plant of Digitaria sanguinalis, identified as Alternaria perpunctulata, and its fermentation broth had an obvious inhibitory effect on the growth of the seedlings of Digitaria sanguinalis. In order to clarify the active substance basis of NBERC_H56, the secondary metabolites of the strain were studied. The results showed that three compounds were obtained by large-scale fermentation, extraction and preparation via high performance liquid chromatography, and were identified as Curvulin （1）, Alternariol （2） and Zinniol （3） by LC-MS and NMR. Alternaria perpunctulata was found to have herbicidal activity for the first time, and its secondary metabolites were isolated and identified for the first time.

Key words: Digitaria sanguinalis, Alternaria, herbicidal activity, secondary metabolites

中图分类号:

S482.4

吴兆圆, 任梦瑶, 张志刚, 刘芳, 张亚妮, 方伟. Alternaria perpunctulata NBERC_H56菌株对马唐的除草活性及其次级代谢产物的分离鉴定[J]. 湖北农业科学, 2021, 60(24): 98-100.

WU Zhao-yuan, REN Meng-yao, ZHANG Zhi-gang, LIU Fang, ZHANG Ya-ni, FANG Wei. Herbicidal activity of Alternaria perpunctulata NBERC_H56 to Digitaria sanguinalis and the secondary metabolites produced by the strain[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(24): 98-100.

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Alternaria perpunctulata NBERC_H56菌株对马唐的除草活性及其次级代谢产物的分离鉴定

Herbicidal activity of Alternaria perpunctulata NBERC_H56 to Digitaria sanguinalis and the secondary metabolites produced by the strain

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