湖北农业科学 ›› 2022, Vol. 61 ›› Issue (24): 84-89.doi: 10.14088/j.cnki.issn0439-8114.2022.24.019

• 植物保护 • 上一篇    下一篇

生防菌BZJN1及化学药剂苯甲·丙环唑对白术根际土壤养分和真菌群落多样性的影响

唐涛, 王帆帆, 郭杰, 郭晓亮, 段媛媛, 游景茂   

  1. 农业农村部中药材生物学与栽培重点实验室/湖北省农业科学院中药材研究所, 湖北 恩施 445000
  • 收稿日期:2022-05-12 出版日期:2022-12-25 发布日期:2023-01-18
  • 通讯作者: 游景茂,副研究员,博士,主要从事中药材病虫害绿色防控技术的研究工作,(电话)13402769227(电子信箱)jingmaoyou@126.com。
  • 作者简介:唐涛(1992-),男,湖北恩施人,助理研究员,硕士,主要从事中药材病虫害绿色防控技术的研究工作,(电话)18071922014(电子信箱)taotang0712@126.com。
  • 基金资助:
    国家中药材产业技术体系项目(CARS-21)

The effects of biocontrol bacteria BZJN1 and chemical agent benzoyl procyclonazole on soil nutrients and fungal community diversity in Atractylodes macrocephala rhizosphere

TANG Tao, WANG Fan-fan, GUO Jie, GUO Xiao-liang, DUAN Yuan-yuan, YOU Jing-mao   

  1. Key Laboratory of Chinese Herbal Medicine Biology and Cultivation, Ministry of Agriculture and Rural Affairs/Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, Hubei, China
  • Received:2022-05-12 Online:2022-12-25 Published:2023-01-18

摘要: 为探究施用生防菌BZJN1和化学药剂苯甲·丙环唑对土壤真菌群落结构的影响,基于真菌ITS高通量测序技术,分析比较化学药剂苯甲·丙环唑处理组和生防菌BZJN1处理组与对照组白术(Atractylodes macrocephala)根际土壤中真菌群落多样性,采用冗余分析(Redundancy analysis, RDA)探讨土壤环境因子与真菌多样性的联系。结果表明,化学药剂苯甲·丙环唑处理组和生防菌BZJN1处理组与对照组白术根际土壤中真菌组成无显著差异,主要优势菌群为子囊菌门(Ascomycota)76.34%、担子菌门(Basidiomycota)12.49%和接合菌门(Zygomycota)3.11%。化学药剂苯甲·丙环唑处理组和生防菌BZJN1处理组白术根际土壤多个真菌门类相对丰度发生显著变化。RDA分析表明,不同环境因子与真菌群落有一定联系,白术根际土壤中3个属真菌群落丰度与pH、过氧化氢酶和速效钾呈显著正相关。

关键词: 白术(Atractylodes macrocephala), 生防菌BZJN1, 苯甲·丙环唑, 真菌群落, 高通量测序

Abstract: In order to explore the effects of biocontrol bacteria BZJN1 and chemical agent benzoyl procyclonazole on soil fungal community structure, based on the fungal ITS high-throughput sequencing technology, the fungal community diversity in rhizosphere soil of Atractylodes macrocephala in chemical agent benzoyl procyclonazole treatment group, biocontrol bacteria BZJN1 treatment group, and the control group was analyzed and compared. Redundancy analysis (RDA) was used to investigate the relationship between soil environmental factors and fungal diversity. The results showed that there was no significant difference in the composition of fungi in rhizosphere soil of Atractylodes macrocephala between the chemical benzoyl·propionazole treatment group, the biocontrol bacteria BZJN1 treatment group and the control group. The dominant bacterial groups were Ascomycota 76.34%, Basidiomycota 12.49% and Zygomycota 3.11%. The relative abundance of fungi in rhizosphere soil of Atractylodes macrocephala was significantly changed in the treatment group of benzoyl · propiconazole and the treatment group of biocontrol bacteria BZJN1. RDA analysis showed that different environmental factors were related to the fungal community to some extent, and the abundance of the fungal communities of the three genera in the rhizosphere soil of Atractylodes macrocephala was positively correlated with pH, catalase and available potassium.

Key words: Atractylodes macrocephala, biocontrol bacteria BZJN1, benzoyl · procyclonazole, fungus community, high-throughput sequencing

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