藤茶复合汤汁抗大鼠血栓功效研究

doi:10.14088/j.cnki.issn0439-8114.2023.09.019

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (9): 101-107.doi: 10.14088/j.cnki.issn0439-8114.2023.09.019

藤茶复合汤汁抗大鼠血栓功效研究

谢勇^1a, 范志平², 施伽^1a, 刘庆庆^1a, 高健强^1b

1.铜仁学院,a材料与化学工程学院;b.梵净山野生动植物资源保护与利用研究中心,贵州铜仁 554300;
2.四川省宜宾市食品药品检验检测中心,四川宜宾 644000

收稿日期:2023-06-06 出版日期:2023-09-25 发布日期:2023-10-24
通讯作者: 高健强（1972-）,男,教授,主要从事作物遗传与育种研究,（电子信箱） gaojianqiang72@126.com。
作者简介:谢勇（1986-）,男,贵州石阡人,副教授,博士,主要从事食品营养与化学研究,（电话）13688564472（电子信箱）747442934@qq.com。
基金资助:
贵州省教育厅青年科技人才成长项目（黔教合KY字[2016]292号）; 贵州省教育厅“一二五”重大科技专项项目（黔教合重大专项字[2012]018号）

Study on anti-thrombotic effect of Ampelopsis grossedentata composite juice in rats

XIE Yong^1a, FAN Zhi-ping², SHI Jia^1a, LIU Qing-qing^1a, GAO Jian-qiang^1b

1a. College of Material and Chemical Engineering; 1b. Research Centre of the Conservation and Utilization of Wildlife Resources in Fanjing Mountain, Tongren University, Tongren 554300, Guizhou, China;
2. Yibin Food and Drug Inspection Testing Center, Yibin 644000, Sichuan, China

Received:2023-06-06 Online:2023-09-25 Published:2023-10-24

摘要/Abstract

摘要： 采用下腔静脉结扎术构建大鼠血栓模型,以藤茶（Ampelopsis grossedentata）复合汤汁进行干预,并以二氢杨梅素及藤茶汤汁为对照。测定大鼠血栓的重量、长度及血栓抑制率,结合HE染色和免疫组化分析血栓样品的理化指标。结果表明,藤茶复合汤汁中总黄酮（1.43±0.11 mg/mL）及二氢杨梅素（0.66±0.05 mg/mL）含量均低于藤茶汤汁和二氢杨梅素溶液的含量。模型组大鼠血栓长度（10.98±1.20 mm）、湿重（70.6±15.44 mg）和干重（9.9±3.2 mg）显著高于正常组。相比于其他干预组,藤茶复合汤汁组的血栓长度（3.92±0.64 mm）和血栓面积占管腔面积百分比（32.1%±1.4%）明显降低,而血栓抑制率（64.5%±1.3%）和溶解率（54.2%±1.3%）显著升高。HE染色及免疫组化分析表明,相比模型组藤茶复合汤汁组大鼠血管细胞完整性损伤程度降低,核固缩现象程度明显减轻,阳性细胞率明显增加,血栓素1相对表达量（25.2%±2.1%）显著降低,说明藤茶复合汤汁具有较好的抗血栓作用。

关键词: 藤茶（Ampelopsis grossedentata）, 二氢杨梅素, 血栓, 血栓素

Abstract: The Ampelopsis grossedentata （AG） composite juice was prepared, followed by investigating its anti-thrombotic effect in rats. The AG composite juice was used to constantly intervene in the thrombus rats model, that was successfully established by using ligation of the inferior vena cava. The DHM solution and AG juice were used as controls. Rats were then sacrificed prior to determining the weight, length of the thrombus samples, and the antithrombotic rate. Consequently, the physicochemical properties of thrombus samples were measured by using HE staining and immunohistochemistry. Results showed that the content of total flavonoids （1.43±0.11 mg/mL） and DHM （0.66±0.05 mg/mL） in composite juice were significantly lower than those in control groups. Moreover, the length （10.98±1.20 mm）, wet weight （70.6±15.4 mg） and dry weight （49.9±3.2 mg） of thrombus in the model group were obviously higher than those in the normal group. Compared with other invention groups, the length of thrombus （3.92±0.64 mm） and the ratio of thrombus area to the entire inner surface area of blood vessels （32.1±1.4）% decreased significantly in the AG composite juice group, while the thrombus inhibition rate （64.5±1.3）% and dissolution rate （54.2±1.3）% increased significantly. Additionally, the HE staining and immunohistochemistry indicated that the AG composite juice group exhibited lower degree of vascular cell damage in integrity and nuclear fixation, relative expression level （25.2±2.1）% of TBXAS1, as well as a higher positive cell rate compared to other intervention groups. The AG composite juice had a favorable anti-thrombotic effect.

Key words: Ampelopsis grossedentata, dihydromyricetin, thrombosis, thromboxane

中图分类号:

Q949.9

谢勇, 范志平, 施伽, 刘庆庆, 高健强. 藤茶复合汤汁抗大鼠血栓功效研究[J]. 湖北农业科学, 2023, 62(9): 101-107.

XIE Yong, FAN Zhi-ping, SHI Jia, LIU Qing-qing, GAO Jian-qiang. Study on anti-thrombotic effect of Ampelopsis grossedentata composite juice in rats[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(9): 101-107.

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藤茶复合汤汁抗大鼠血栓功效研究

Study on anti-thrombotic effect of Ampelopsis grossedentata composite juice in rats

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