白簕根皮乙酸乙酯部位镇痛抗炎及抗氧化活性研究

doi:10.14088/j.cnki.issn0439-8114.2023.12.023

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (12): 130-134.doi: 10.14088/j.cnki.issn0439-8114.2023.12.023

白簕根皮乙酸乙酯部位镇痛抗炎及抗氧化活性研究

梁资就, 蒋林, 林青华, 林昌源, 张仁义, 黄小洪, 赖信宏, 谢孟姣, 陈海燕, 李芳婵

广西中医药大学/广西高校中药制剂共性技术研发重点实验室/广西壮瑶药工程技术研究中心,南宁 530200

收稿日期:2022-07-13 发布日期:2024-01-10
通讯作者: 李芳婵,女,实验师,硕士,主要从事中药、食品研究与开发工作,（电子信箱）1936490308@qq.com。
作者简介:梁资就（1999-）,男,广西贵港人,在读硕士研究生,研究方向为中药、保健食品研究与开发,（电话）13321773526（电子信箱）1823299106@qq.com。
基金资助:
广西科技计划项目重点研发计划项目（桂科AB1850022; 桂科AB21196051）; 2019 年广西中医药大学校级科研项目（2019QN007）

Analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction of root bark of Acanthopanax trifoliatus

LIANG Zi-jiu, JIANG Lin, LIN Qing-hua, LIN Chang-yuan, ZHANG Ren-yi, HUANG Xiao-hong, LAI Xin-hong, XIE Meng-jiao, CHEN Hai-yan, LI Fang-chan

Guangxi University of Chinese Medicine/Guangxi University Key Laboratory of Common Technology Research and Development of Traditional Chinese Medicine Preparation/Guangxi Zhuang and Yao Medicine Engineering Technology Research Center, Nanning 530200, China

Received:2022-07-13 Published:2024-01-10

摘要/Abstract

摘要： 为研究白簕[Acanthopanax trifoliatus （L.） Merr]根皮乙酸乙酯部位（BLE）镇痛抗炎及抗氧化能力,以BLE为研究对象,采用热板致痛法、醋酸扭体法、二甲苯致小鼠耳肿胀法、角叉菜胶致小鼠足肿胀法及前列腺素E₂（PGE₂）炎症介质含量测定探讨其镇痛、抗炎作用,通过DPPH法和ABTS法评价BLE的抗氧化能力。结果表明,与空白组相比,BLE溶液各剂量组给药60 min时痛阈值均显著或极显著提高,与给药前相比,各剂量组在各时间点痛域值均显著或极显著提高,各剂量组与雷公藤多苷片组间无显著性差异。与空白组相比,各剂量组小鼠扭体次数极显著减少;各剂量组小鼠对二甲苯所致耳肿胀度均有显著或极显著减轻,且各剂量组与雷公藤多苷片组间无显著差异;高剂量组小鼠对角叉菜胶致足肿胀度有极显著减轻,各剂量组与雷公藤多苷片组相比无显著差异。与空白组相比,高、中剂量组小鼠PGE₂含量极显著降低;与雷公藤多苷片组相比,高、中剂量组PGE₂含量均无显著差异。BLE清除PDDH自由基的清除率随浓度增加呈正相关,IC₅₀为0.029 mg/mL,低于阳性对照维生素C的IC₅₀（0.04 mg/mL）,ABTS自由基清除率随浓度增加呈正相关,IC₅₀为0.033 mg/mL,低于阳性对照维生素C的IC₅₀（0.042 mg/mL）。

关键词: 白簕[Acanthopanax trifoliatus （L.） Merr]根皮, 乙酸乙酯部位, 镇痛, 抗炎, 抗氧化

Abstract: In order to study the analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction from the root bark of Acanthopanax trifoliatus（BLE）, the analgesia and anti-inflammatory effects of BLE were investigated by hot plate pain method, acetic acid writhing method, xylene-induced mouse ear swelling method, carrageenan-induced mouse paw swelling method and prostaglandin E₂（PGE₂） content determination. The antioxidant activities of BLE were evaluated by DPPH free radical method and ABTS free radical method. The results showed that compared with the blank group, the pain threshold of each BLE dose group at 60 min was significantly or extremely significantly increased, compared with before medication, the pain threshold of each dose group at each time point was significantly or extremely significantly increased and the difference in the pain threshold of each dose group and tripterygium glycosides tablet group were not significantly indigenous. Compared with the blank group, the writhing times of mice in each dose group were extremely significantly reduced; each dose group could significantly or extremely significantly reduce the ear swelling induced by xylene and there was no significant difference in ear swelling between each dose group and tripterygium glycosides tablets group; the high-dose group could extramely significantly reduce the paw swelling induced by carrageenan, and compared with the tripterygium glycosides tablets group, the paw swelling degrees in each dose group showed no significant difference. Compared with the blank group, the content of PGE₂ in high-dose and medium-dose groups was extremely significantly decreased. Compared with the tripterygium glycoside tablet group, there was no significant difference in PGE₂ content in high and medium dose groups. The clearance rate of PDDH free radical by BLE was positively correlated with the increase of concentration, with IC₅₀ of 0.029 mg/ml, which was lower than that of the positive control vitamin C （0.04 mg/mL）. The clearance rate of ABTS free radical was positively correlated with the increase of concentration, with IC₅₀ of 0.033 mg/mL, which was lower than that of the positive control vitamin C （0.042 mg/mL）.

Key words: root bark of Acanthopanax trifoliatus, ethyl acetate fraction, analgesia, anti-inflammatory, antioxidation

中图分类号:

R284
R285

梁资就, 蒋林, 林青华, 林昌源, 张仁义, 黄小洪, 赖信宏, 谢孟姣, 陈海燕, 李芳婵. 白簕根皮乙酸乙酯部位镇痛抗炎及抗氧化活性研究[J]. 湖北农业科学, 2023, 62(12): 130-134.

LIANG Zi-jiu, JIANG Lin, LIN Qing-hua, LIN Chang-yuan, ZHANG Ren-yi, HUANG Xiao-hong, LAI Xin-hong, XIE Meng-jiao, CHEN Hai-yan, LI Fang-chan. Analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction of root bark of Acanthopanax trifoliatus[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 130-134.

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白簕根皮乙酸乙酯部位镇痛抗炎及抗氧化活性研究

Analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction of root bark of Acanthopanax trifoliatus

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