酸性染料比色法测定对叶百部中总生物碱含量

doi:10.14088/j.cnki.issn0439-8114.2023.12.028

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (12): 154-157.doi: 10.14088/j.cnki.issn0439-8114.2023.12.028

酸性染料比色法测定对叶百部中总生物碱含量

吴港圆^1a, 覃海琴^1a, 胡东南², 张淼^1b, 谢凤凤^1b, 王孝勋^1a

1a.广西中医药大学,药学院,南宁 530200;
1b.广西中医药大学,广西壮瑶药重点实验室,南宁 530200;
2.广西壮族自治区药用植物园,南宁 530023

收稿日期:2022-07-11 发布日期:2024-01-10
通讯作者: 王孝勋（1973-）,男,湖南常德人,研究员,博士,主要从事中药品种、质量及资源开发研究,（电话）15977781099（电子信箱）407977122@qq.com。
作者简介:吴港圆（1997-）,女（侗族）,湖南通道人,在读硕士研究生,研究方向为叶百部野生及迁地栽培品种种质资源开发,（电话）15874578597（电子信箱）2482293367@qq.com。
基金资助:
广西壮瑶药重点实验室基金资助项目（桂科基字[2014]32号）; 广西2011协同创新中心基金资助项目（桂教科研[2013]20号）; 广西南宁市科学研究与技术开发计划基金资助项目（20193120）

Determination of total alkaloid in Stemona tuberosa Lour. by acid dye colorimetric method

WU Gang-yuan^1a, QIN Hai-qin^1a, HU Dong-nan², ZHANG Miao^1b, XIE Feng-feng^1b, WANG Xiao-xun^1a

1a. College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200, China;
1b. Key Laboratory of Zhuang Yao Medicine in Guangxi,Guangxi University of Chinese Medicine,Nanning 530200, China;
2. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China

Received:2022-07-11 Published:2024-01-10

摘要/Abstract

摘要： 采用酸性染料比色法测定不同发育时期的对叶百部（Stemona tuberosa Lour.）中总生物碱含量,采用酸性染料比色法,以对叶百部碱为对照品,溴麝香草酚蓝为酸性染料,甲醇、三氯甲烷提取,4%盐酸溶解,在413 nm 波长下测定。结果表明,对叶百部碱的标准曲线为Y=0.003 7X+0.089 （R²=0.995 9）,在32~800 μg/mL范围内线性关系良好;平均加样回收率为101.23%,平均加样回收率的RSD为2.63%。1年生对叶百部总生物碱含量为1.87~4.55 mg/g;2年生对叶百部总生物碱含量为4.52~9.63 mg/g。酸性染料比色法具有操作简便、重复性好、稳定性高的优点。

关键词: 对叶百部（Stemona tuberosa Lour.）, 总生物碱, 酸性染料比色法, 不同发育时期

Abstract: The total alkaloid content in Stemona tuberosa Lour. at different developmental stages was determined using the acid dye colorimetric method. The acid dye colorimetric method was used, with Stemona tuberosa Lour. alkali as the reference material and bromothymol blue as the acid dye. Methanol and chloroform were extracted, and dissolved in 4% hydrochloric acid. The determination was carried out at a wavelength of 413 nm. The results showed that the standard curve of Stemona tuberosa Lour. alkali was Y=0.003 7X+0.089 （R²=0.995 9）. The linear relationship was good from 32 to 800 μg/mL;the average sample recovery rate was 101.23%, and the RSD of the average sample recovery rate was 2.63%. The total alkaloid content of annual Stemona tuberosa Lour. was 1.87~4.55 mg/g;the total alkaloid content of the biennial Stemona tuberosa Lour. was 4.52~9.63 mg/g. The acid dye colorimetric method had the advantages of simple operation, good repeatability, and high stability.

Key words: Stemona tuberosa Lour, total alkaloid, acid dye colorimetric method, different developmental stages

中图分类号:

R917

吴港圆, 覃海琴, 胡东南, 张淼, 谢凤凤, 王孝勋. 酸性染料比色法测定对叶百部中总生物碱含量[J]. 湖北农业科学, 2023, 62(12): 154-157.

WU Gang-yuan, QIN Hai-qin, HU Dong-nan, ZHANG Miao, XIE Feng-feng, WANG Xiao-xun. Determination of total alkaloid in Stemona tuberosa Lour. by acid dye colorimetric method[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 154-157.

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酸性染料比色法测定对叶百部中总生物碱含量

Determination of total alkaloid in Stemona tuberosa Lour. by acid dye colorimetric method

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