猪NARS2基因变异与硒都黑猪胴体和肉质性状的关联分析

doi:10.14088/j.cnki.issn0439-8114.2023.12.034

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (12): 195-199.doi: 10.14088/j.cnki.issn0439-8114.2023.12.034

猪NARS2基因变异与硒都黑猪胴体和肉质性状的关联分析

乔同同, 张宇, 李梓芃, 乔木, 秦振洋, 彭先文, 吴俊静

湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉 430064

收稿日期:2023-09-22 发布日期:2024-01-10
通讯作者: 吴俊静（1984-）,女,研究员,博士,主要从事猪遗传育种研究,（电话）027-87389516（电子信箱）jeanne1106@126.com。
作者简介:乔同同（2001-）,男,河南商丘人,在读本科生,专业方向为动物科学,（电话）027-87389516（电子信箱）qtt16162630@163.com。
基金资助:
湖北省重大科技创新项目（2022BBA0018）; 湖北洪山实验室重大项目（2021hszd003）; 国家生猪产业技术体系项目（CARS-36）; 湖北省农业科技创新中心创新团队项目（2021-620-000-001-018）

Association analysis of NARS2 gene variation and carcass and meat quality traits in selenium black pig

QIAO Tong-tong, ZHANG Yu, LI Zi-peng, QIAO Mu, QIN Zhen-yang, PENG Xian-wen, WU Jun-jing

Institute of Animal Science and Veterinary Medicine, Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Hubei Academy of Agricultural Sciences, Wuhan 430064,China

Received:2023-09-22 Published:2024-01-10

摘要/Abstract

摘要： 在硒都黑猪群体中发现猪NARS2基因第9内含子中存在g.101284 C>T突变位点,采用PCR产物直接测序法进行基因分型后,与23个胴体和肉质类性状表型进行关联分析。结果表明,NASR2基因g.101284 C>T位点极显著影响猪胴体皮厚（P<0.01）,且显著影响猪肉眼肌面积、滴水损失48 h和肌内脂肪含量性状（P<0.05）。CC基因型个体皮厚极显著低于TT基因型个体（P<0.01）;CC基因型个体滴水损失48 h和肌内脂肪含量显著高于其他基因型个体,而TT基因型个体胴体眼肌面积显著小于其他基因型个体（P<0.05）。而这4个性状中,除皮厚与眼肌面积性状不存在显著相关外,其他各性状之间存在一定程度的相关性。其中,滴水损失48 h与肌内脂肪含量性状相关性最高（r²=0.882,P<0.01）。因此,该标记在育种应用中应当结合育种目标合理利用,针对不同主选性状,选留或淘汰不同基因型个体。

关键词: 硒都黑猪, 胴体性状, 肉质性状, NARS2基因, 关联分析

Abstract: A g.101284 C>T mutation site was found in intron 9 of the NARS2 gene in the selenium black pig population. After genotyping using PCR product direct sequencing, association analysis was conducted with 23 phenotypes of carcass and meat quality traits.The results showed that the g.101284 C>T locus of the NASR2 gene significantly affected the skin thickness of pig carcasses （P<0.01）, and significantly affected the eye muscle area, drip loss for 48 hours, and intramuscular fat content traits of pork （P<0.05）. The skin thickness of individuals with CC genotype was significantly lower than that of individuals with TT genotype （P<0.01）;the 48 hours drip loss and intramuscular fat content of individuals with CC genotype were significantly higher than those with other genotypes, while the carcass eye muscle area of individuals with TT genotype was significantly smaller than that of individuals with other genotypes （P<0.05）. Among these four personality traits, there was a certain degree of correlation between other traits except for skin thickness and eye muscle area traits, which were not significantly correlated. Among them, the 48 hours drip loss had the highest correlation with intramuscular fat content traits （r²=0.882, P<0.01）. Therefore, this marker should be reasonably utilized in breeding applications in conjunction with breeding objectives, and individuals of different genotypes should be selected or eliminated for different main selected traits.

Key words: selenium black pig, carcass traits, meat quality traits, NARS2 gene, association analysis

中图分类号:

S828
S813

乔同同, 张宇, 李梓芃, 乔木, 秦振洋, 彭先文, 吴俊静. 猪NARS2基因变异与硒都黑猪胴体和肉质性状的关联分析[J]. 湖北农业科学, 2023, 62(12): 195-199.

QIAO Tong-tong, ZHANG Yu, LI Zi-peng, QIAO Mu, QIN Zhen-yang, PENG Xian-wen, WU Jun-jing. Association analysis of NARS2 gene variation and carcass and meat quality traits in selenium black pig[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 195-199.

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猪NARS2基因变异与硒都黑猪胴体和肉质性状的关联分析

Association analysis of NARS2 gene variation and carcass and meat quality traits in selenium black pig

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