关键词: 高效液相色谱法, 果蔬, 有机酸, 维生素C, 含量

Abstract: Using a 1% oxalic acid （containing 10% methanol） solution to extract organic acids （tartaric acid, citric acid, malic acid, succinic acid, fumaric acid） and VC from fruits and vegetables,the chromatographic column was InertSustain C18 （250 mm×4.6 mm, 5 μm）, with 0.1% phosphoric acid aqueous solution as mobile phase A and methanol as mobile phase B, gradient elution, injection volume of 20 μL, column temperature of 30 ℃, and flow rate of 0.8 mL/min. The content of 5 organic acids and was detected VC at wavelengths of 210 nm and 254 nm, respectively. The results showed that the method had a good linear relationship within the standard curve range, with correlation coefficients （R²） all greater than 0.999 0, detection limits of 0.01~0.90 μg/mL, quantification limits of 0.15~5.00 μg/mL, relative standard deviation of 2.40%~3.75%, and recovery rates of 86.4%~115.2%;this method had the advantages of fast analysis speed, high sensitivity, good repeatability, and high efficiency, and could be used for the determination of organic acids and VC content in fruits and vegetables.

Key words: high performance liquid chromatography, fruits and vegetables, organic acids, VC, content