山豆根菌核病病原菌鉴定及室内药剂筛选

doi:10.14088/j.cnki.issn0439-8114.2024.09.016

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (9): 89-94.doi: 10.14088/j.cnki.issn0439-8114.2024.09.016

山豆根菌核病病原菌鉴定及室内药剂筛选

宋利沙, 蒋妮, 张占江, 韦树根, 丘卓秋, 黄琦, 詹鑫婕, 潘丽梅

广西壮族自治区药用植物园/广西道地药材高品质形成与应用重点实验室/广西中药材良种繁育技术创新中心/国家中医药传承创新中心,南宁 530023

收稿日期:2023-06-19 出版日期:2024-09-25 发布日期:2024-09-30
通讯作者: 蒋妮,（电子信箱）jiangni292@126.com。
作者简介:宋利沙（1987-）,女,河南淇县人,副研究员,博士,主要从事药用植物病虫害防治工作,（电话）18587575152（电子信箱）lishasong@126.com。
基金资助:
广西中医药适宜技术开发与推广项目（GZSY21-03）; 广西卫生健康委员会重点实验室建设项目（ZJC2020003）; 中医药多学科交叉创新团队项目（GZKJ2305）

Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents

SONG Li-sha, JIANG Ni, ZHANG Zhan-jiang, WEI Shu-gen, QIU Zhuo-qiu, HUANG Qi, ZHAN Xin-jie, PAN Li-mei

Guangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-Di Herbs/Guangxi Traditional Chinese Medicine Breeding Technology Innovation Center/ National Center for Traditional Chinese Medicine Inheritance and Innovation, Nanning 530023, China

Received:2023-06-19 Published:2024-09-25 Online:2024-09-30

摘要/Abstract

摘要： 采用常规组织分离法分离山豆根（Sophora tonkinensis Radix）菌核病病原菌,利用柯赫式法则进行致病性测定,基于形态特征、菌丝融合群测定、细胞核荧光染色以及rDNA-ITS序列分析进行病原菌的鉴定,并利用菌丝生长速率法测定5种杀菌剂对病原菌的抑制活性。结果表明,山豆根菌核病的病原菌为立枯丝核菌（Rhizoctonia solani）,属于融合群AG-1。98%恶霉灵可溶性粉剂（SP）、75%肟菌·戊唑醇水分散粒剂（WG）、250 g/L吡唑醚菌酯乳油（EC）、10亿/g哈茨木霉菌悬浮剂（SC）、50%多菌灵可湿性粉剂（WP）对病原菌菌丝的生长均有较好的抑制效果,抑制中浓度（EC₅₀）分别为0.061 1、0.575 7、0.074 8、0.284 3、0.740 5 mg/L。

关键词: 山豆根（Sophora tonkinensis Radix）, 菌核病, 病原菌鉴定, 室内药剂筛选, 立枯丝核菌（Rhizoctonia solani）

Abstract: The pathogens from Sophora tonkinensis Radix were isolated by conventional tissue separation method, the pathogenicity was determined by Koch's formula, and the pathogens were identified based on morphological characteristics, mycelium fusion group determination, nuclear fluorescence staining and rDNA-ITS sequence analysis. The inhibitory activities of 5 fungicides against pathogens were measured by the mycelium growth rate method. The results showed that the pathogen was Rhizoctonia solani, belonging to the fusion group AG-1. 98% hymexazol soluble power （SP）, 75% oxime ·pentazolol water dispersible granule （WG）, 250 g/L pyraclostrobin emulsifiable concentrate （EC）, 1 billion /g Trichoderma harziana suspension concentrate （SC）, and 50% carbendazim wettable power （WP） all had good inhibitory effects on the growth of pathogenic fungi mycelia. The inhibitory medium effective concentrations （EC₅₀） were 0.061 1, 0.575 7, 0.074 8, 0.284 3, 0.740 5 mg/L, respectively.

Key words: Sophora tonkinensis Radix, sclerotinia, identification of pathogen, screening of laboratory agents, Rhizoctonia solani

中图分类号:

宋利沙, 蒋妮, 张占江, 韦树根, 丘卓秋, 黄琦, 詹鑫婕, 潘丽梅. 山豆根菌核病病原菌鉴定及室内药剂筛选[J]. 湖北农业科学, 2024, 63(9): 89-94.

SONG Li-sha, JIANG Ni, ZHANG Zhan-jiang, WEI Shu-gen, QIU Zhuo-qiu, HUANG Qi, ZHAN Xin-jie, PAN Li-mei. Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(9): 89-94.

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山豆根菌核病病原菌鉴定及室内药剂筛选

Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents

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