饲料添加牡丹籽壳对黄颡鱼肝脏组织学、抗氧化酶活性和非特异性免疫的影响

doi:10.14088/j.cnki.issn0439-8114.2024.10.023

摘要/Abstract

摘要： 为探究饲料中添加牡丹籽壳对黄颡鱼（Pelteobagrus fulvidraco）抗氧化能力和非特异性免疫的影响,以饲喂商品饲料的黄颡鱼为空白对照组,以饲喂含有1%和5%牡丹籽油的再制商品饲料的黄颡鱼为阳性对照组,以饲喂含有1%和5%牡丹籽壳的再制商品饲料的黄颡鱼为试验组,养殖时间21 d。测定黄颡鱼血清和肝脏抗氧化酶活性、血清溶菌酶活性及补体C3含量,并在光学显微镜下观察黄颡鱼肝脏组织形态学结构。结果表明,与对照组相比,饲料添加1%牡丹籽壳显著提高黄颡鱼血清中超氧化物歧化酶活性（P<0.001）和补体C3（P<0.05）含量,黄颡鱼肝脏中超氧化物歧化酶和过氧化氢酶活性显著升高（P<0.001）,指示肝脏氧化损伤程度的丙二醛含量显著降低（P<0.001）,黄颡鱼肝脏形态结构完好,与对照组无明显差异。牡丹籽壳提高黄颡鱼抗氧化能力和非特异性免疫的效果在5%剂量添加组更加显著,与空白对照组相比,血清超氧化物歧化酶、肝脏超氧化物歧化酶和过氧化氢酶活性分别提高了100%（P<0.001）、245%（P<0.001）和176%（P<0.001）,血清溶菌酶活性和补体C3含量分别提高了36%（P<0.001）和33%（P<0.01）。与牡丹籽油相比,饲料添加牡丹籽壳能达到与其相同的保健效果。饲料添加牡丹籽壳能有效提高黄颡鱼抗氧化能力和非特异性免疫,适宜添加量为1%~5%。

关键词: 牡丹籽壳, 牡丹籽油, 饲料添加剂, 抗氧化, 非特异性免疫, 黄颡鱼（Pelteobagrus fulvidraco）

Abstract: To investigate the effects of dietary peony seed shell on fish oxidation resistance and nonspecific immunity, yellow catfish were fed with 1% or 5% peony seed shell （commercial feed containing 1% or 5% peony seed shell） for 3 weeks. Meanwhile, fish fed with commercial feed only were used as the blank control group, and fish fed with 1% or 5% peony seed oil were used as positive control group. The results showed that, compared with blank control group, the activity of superoxide dismutase （P<0.001） and the content of complement C3 （P<0.05） in serum of fish fed with 1% peony seed shell increased significantly. Liver superoxide dismutase and catalase activities were higher than those of the blank control group （P<0.001）, and liver malondialdehyde content decreased significantly （P<0.001）. Liver histomorphology of yellow catfish was normal and intact in both the blank control group and 1% peony seed shell group, and there was no obvious difference between the two groups. Peony seed shell had a dose-dependent positive effect on promoting the antioxidant capacity and nonspecific immunity in yellow catfish, all the test indexes in the 5% peony seed shell group showed a remarkably better change. Compared with blank control group, serum superoxide dismutase, liver superoxide dismutase and catalase activities in 5% peony seed shell group increased by 100% （P<0.001）, 245% （P<0.001） and 176% （P<0.001）, respectively. Serum lysozyme activity and complement C3 content increased by 36% （P<0.001） and 33% （P<0.01）, respectively. In both dose groups, peony seed shell showed similar positive effects on yellow catfish compared to peony seed oil, the suitable addition amount was 1%~5%.

Key words: peony seed shell, peony seed oil, feed additive, antioxidation, nonspecific immunity, Pelteobagrus fulvidraco

中图分类号:

S963.73

刘鹿忆, 王湛昌, 朱文欢, 何弯弯, 艾桃山, 张立强. 饲料添加牡丹籽壳对黄颡鱼肝脏组织学、抗氧化酶活性和非特异性免疫的影响[J]. 湖北农业科学, 2024, 63(10): 124-130.

LIU Lu-yi, WANG Zhan-chang, ZHU Wen-huan, HE Wan-wan, AI Tao-shan, ZHANG Li-qiang. Effects of dietary peony seed shell on the liver histopathology, antioxidant activity and nonspecific immunity of Pelteobagrus fulvidraco[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(10): 124-130.

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