基于叶绿体基因组的八角枫属及近缘属植物系统发育分析

doi:10.14088/j.cnki.issn0439-8114.2025.01.027

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (1): 168-173.doi: 10.14088/j.cnki.issn0439-8114.2025.01.027

基于叶绿体基因组的八角枫属及近缘属植物系统发育分析

杨玉霞¹, 李静婷¹, 李颖¹, 苏晨^2,3, 闫睿¹, 魏泽昶¹

1.兰州交通大学生物与制药工程学院,兰州 730070;
2.平顶山学院河南省生态经济型木本植物种质创新与利用重点实验室,河南平顶山 467000;
3.首都师范大学资源环境与旅游学院,北京 100048

收稿日期:2024-01-09 发布日期:2025-02-20
通讯作者: 李静婷（1983-）,女,内蒙古包头人,副教授,博士,主要从事功能基因组学研究,（电子信箱）jingting_lee@163.com。
作者简介:杨玉霞（2000-）,女,甘肃徽县人,在读硕士研究生,研究方向为药用植物基因组与功能基因组学,（电话）18794135446（电子信箱）3250740174@qq.com。
基金资助:
国家自然科学基金项目（32360484）; 河南省高等学校重点科研项目（22B210008）; 大学生创新创业训练计划项目（CXXL20230184）

Analysis of systemic development in the genus Alangium chinense and its related genera plants based on chloroplast genome

YANG Yu-xia¹, LI Jing-ting¹, LI Ying¹, SU Chen^2,3, YAN Rui¹, WEI Ze-chang¹

1. College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Henan Key Laboratory of Eco-economic Woody Plant Germplasm Innovation and Utilization, Pingdingshan University, Pingdingshan 467000, Henan, China;
3. College of Resource Environment and Tourism, Capital Normal University, Beijing 100048, China

Received:2024-01-09 Online:2025-02-20

摘要/Abstract

摘要： 为明确八角枫（Alangium chinense （Lour.） Harms）叶绿体基因组的结构特点及系统发育关系,通过Illumina高通量测序技术对八角枫叶绿体基因组进行测序,并进行组装、注释和结构分析。利用mVISTA和Geneious软件对八角枫、毛八角枫、高山八角枫、八角枫MG524996和八角枫NC044840叶绿体基因组进行同源比对和共线性分析,采用MEGA 11软件构建植物系统发育树。结果表明,八角枫完整的叶绿体基因组全长156 672 bp,呈双链环状结构,GC占37.69%;叶绿体基因组共有130个编码基因,包括81个蛋白质编码基因（PCGs）、38个tRNA转运基因、8个rRNA基因和3个假基因,其在叶绿体基因组序列中占比分别为62.31%、29.23%、6.15%和2.31%。同源比对和共线性分析发现,5种植物（八角枫、其他4种八角枫属植物）叶绿体基因组间具有高度相似性和共线性。系统发育分析表明,八角枫与高山八角枫亲缘关系最为接近,暗示他们在进化上具有共线性,推测该八角枫为新亚种。

关键词: 叶绿体基因组, 八角枫属, 近缘属植物, 系统发育

Abstract: In order to clarify the structural characteristics and phylogenetic relationships of the chloroplast genome of Alangium chinense （Lour.） Harms, Illumina high-throughput sequencing technology was used to sequence the chloroplast genome of Alangium chinense, followed by assembly, annotation, and structural analysis. Using mVISTA and Geneous software, homologous alignment and collinearity analysis were performed on the chloroplast genome of Alangium chinense, Alangium kurzii Craib, Alangium alpinum, Alangium chinense MG524996, and Alangium chinense NC044840. A plant phylogenetic tree was constructed using MEGA 11 software. The results showed that the complete chloroplast genome of Alangium chinense was 156 672 bp in length, with a double-stranded circular structure and a GC proportion of 37.69%;the chloroplast genome consists of 130 coding genes, including 81 protein coding genes （PCGs）, 38 tRNA transport genes, 8 rRNA genes, and 3 pseudogenes, accounting for 62.31%, 29.23%, 6.15%, and 2.31% of the chloroplast genome sequence, respectively. Through homology comparison and collinearity analysis, it was found that the chloroplast genome of five plants （Alangium chinense and four other plants in the Alangium chinense genus） exhibited high similarity and collinearity.Phylogenetic analysis indicated that Alangium chinense was most closely related Alangium alpinum, suggesting their collinearity in evolution. It was speculated that Alangium chinense was a new subspecies.

Key words: chloroplast genome, Alangium chinense genus, related genera plants, systemic development

中图分类号:

R931

杨玉霞, 李静婷, 李颖, 苏晨, 闫睿, 魏泽昶. 基于叶绿体基因组的八角枫属及近缘属植物系统发育分析[J]. 湖北农业科学, 2025, 64(1): 168-173.

YANG Yu-xia, LI Jing-ting, LI Ying, SU Chen, YAN Rui, WEI Ze-chang. Analysis of systemic development in the genus Alangium chinense and its related genera plants based on chloroplast genome[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(1): 168-173.

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基于叶绿体基因组的八角枫属及近缘属植物系统发育分析

Analysis of systemic development in the genus Alangium chinense and its related genera plants based on chloroplast genome

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