湖北农业科学 ›› 2025, Vol. 64 ›› Issue (3): 138-143.doi: 10.14088/j.cnki.issn0439-8114.2025.03.022

• 贮藏·加工 • 上一篇    下一篇

微波辅助提取松乳菇多糖及其抗氧化性研究

江舒, 郑玉凤, 吴芳, 张锦凡, 熊佳怡, 胡婷   

  1. 黄冈师范学院生物与农业资源学院/经济林木种质改良与资源综合利用湖北省重点实验室/大别山特色资源开发湖北省协同创新中心,湖北 黄冈 438000
  • 收稿日期:2024-05-21 发布日期:2025-04-17
  • 通讯作者: 胡 婷(1988-),女,湖北仙桃人,副教授,博士,主要从事食品大分子的提取与生物活性研究,(电话)13419602772(电子信箱)843076915@qq.com。
  • 作者简介:江 舒(2003-),女,湖北黄陂人,在读本科生,专业方向为食品大分子的提取与生物活性,(电话)19986830630(电子信箱)3091183503@qq.com。
  • 基金资助:
    黄冈师范学院高级别培育项目(201816703)

Research on microwave-assisted extraction of polysaccharide from Lactarius delicious and its antioxidant activity

JIANG Shu, ZHENG Yu-feng, WU Fang, ZHANG Jin-fan, XIONG Jia-yi, HU Ting   

  1. College of Biology and Agricultural Resources, Huanggang Normal University/Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization/Hubei Collaborative Innovation Center for the Characteristice Resources Exploitation of Dabie Mountains, Huanggang 438000,Hubei, China
  • Received:2024-05-21 Online:2025-04-17

摘要: 以松乳菇(Lactarius deliciosus)为原材料,选取料液比、微波功率、微波时间、水浴温度、水浴时间5个变量进行单因素试验,利用响应面分析对微波辅助提取松乳菇多糖工艺进行优化,并对其抗氧化性进行研究。结果表明,最佳提取工艺条件为料液比1∶35 g/mL、微波功率640 W、微波时间25 s、水浴温度90 ℃、水浴时间120 min,在该条件下松乳菇多糖的提取率可达(4.61±0.03)%;松乳菇多糖具有良好的抗氧化性,当松乳菇多糖的质量浓度为1.0 mg/mL时,其总还原能力和DPPH自由基清除率达到最大。

关键词: 松乳菇(Lactarius deliciosus), 微波辅助提取, 多糖, 抗氧化活性, 响应面分析

Abstract: Lactarius delicious was used as raw material, the material-liquid ratio, microwave power, microwave time, water bath temperature, and water bath time were selected as the variables for the single-factor test, and the Lactarius delicious polysaccharide microwave-assisted extraction process was optimized by using response surface analysis, and its antioxidant activity was studied. The results showed that the best extraction process condition was the material-liquid ratio of 1∶35 g/mL, microwave power of 640 W, microwave time of 25 s, water bath temperature of 90 ℃, and water bath time of 120 min. Under this condition, the extraction rate of polysaccharide from Lactarius delicious was (4.61±0.03)%. The polysaccharide from Lactarius delicious had good antioxidant activity. When the mass concentration of polysaccharide from Lactarius delicious was 1.0 mg/mL, its total reducing ability and DPPH radical scavenging rate reached the maximum.

Key words: Lactarius delicious, microwave-assisted extraction, polysaccharides, antioxidant activity, response surface analysis

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