湖北农业科学 ›› 2025, Vol. 64 ›› Issue (5): 83-89.doi: 10.14088/j.cnki.issn0439-8114.2025.05.013

• 药用植物 • 上一篇    下一篇

桑寄生种子萌发过程中的生理生化变化

冯长存1, 梁露2, 梁文静3, 桂凌健3, 宋利沙3, 潘丽梅3, 付金娥3, 万凌云3, 韦树根1,3   

  1. 1.广西医科大学药学院,南宁 530021;
    2.广西中医药大学研究生院,南宁 530200;
    3.广西壮族自治区药用植物园/广西道地药材高品质形成与应用重点实验室,南宁 530023
  • 收稿日期:2024-09-12 出版日期:2025-05-25 发布日期:2025-06-11
  • 通讯作者: 万凌云(1984-),男,江西上饶人,副研究员,博士,主要从事中药资源与分子生药学研究,(电子信箱)wanlingyun1984@163.com;韦树根(1980-),男,广西钦州人,研究员,博士,主要从事药用植物栽培与遗传育种研究,(电子信箱)weishugen2@163.com。
  • 作者简介:冯长存(1997-),男,山东临沂人,在读硕士研究生,研究方向为药用植物栽培与遗传育种,(电子信箱)fchangcun@163.com
  • 基金资助:
    国家自然科学基金项目(82173933; 81960695); 广西自然科学基金项目(2022GXNSFAA035557; 2024GXNSFAA010371); 广西中医药管理局自筹课题(GXZYA20230014)

Physiological and biochemical studies on germination dynamics of Taxillus chinensis seeds

FENG Chang-cun1, LIANG Lu2, LIANG Wen-jing3, GUI Ling-jian3, SONG Li-sha3, PAN Li-mei3, FU Jin-e3, WAN Ling-yun3, WEI Shu-gen1,3   

  1. 1. School of Pharmacy, Guangxi Medical University, Nanning 530021, China;
    2. Graduate College, Guangxi University of Chinese Medicine, Nanning 530200, China;
    3. Guangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of High-quality Formation and Utilization of Dao-di Herbs, Nanning 530023, China
  • Received:2024-09-12 Published:2025-05-25 Online:2025-06-11

摘要: 以带果胶的桑寄生(Taxillus chinensis)种子作为对照,去果胶的桑寄生种子作为处理,分别在培养0、5、10、15、20、25 d时取样,测定样品相关生理生化指标并比较分析。结果表明,对照多糖含量呈先升后降趋势,而去果胶处理呈下降趋势;两组中的可溶性糖含量均呈下降-上升-下降的变化趋势,且在整个萌发过程中,去果胶处理的可溶性糖含量显著低于对照。对照MDA含量呈上升趋势,去果胶处理呈下降-上升-下降的变化趋势,且在萌发过程中的大部分时期去果胶处理低于对照;两组中H2O2和O2-的含量变化趋势相似,但在萌发过程中的大部分时期去果胶处理的含量高于对照。SOD、POD、PAL三者活性在两组中的变化趋势相似,去果胶处理SOD活性始终高于对照,去果胶处理POD和PAL活性虽低于对照,但持续呈上升趋势。内源激素含量(IAA、ABA、GA3、ZR)在两组中均呈波动性变化,对照总体呈下降趋势,而去果胶处理则明显增加且总体呈上升趋势。初步证实了去果胶能够促使桑寄生种子贮藏物质、酶及内源激素等的快速调动,从而促进种子的顺利萌发。

关键词: 桑寄生(Taxillus chinensis), 种子, 果胶, 萌发过程, 生理生化指标

Abstract: Using Taxillus chinensis seeds with pectin as the control group and de-pectinated seeds as the treated group, samples were collected at 0, 5, 10, 15, 20, and 25 days of cultivation. Then relevant physiological and biochemical indicators were determined and comparatively analyzed. The results showed that the polysaccharide content in the control exhibited an initial increase and then followed by a decrease, while the de-pectinated treatment showed a continuous declining trend. The soluble sugar content in both groups displayed a decrease-increase-decrease pattern, with significantly lower levels observed in the de-pectinated treatment compared to the control throughout the germination process. The MDA content in the control group showed an upward trend, while in the de-pectinated group, a downward-upward-downward trend was exhibited and it was mostly lower than that in the control during germination. The content of H2O2 and O2- showed similar trends in both groups, but the content in the de-pectinated group was mostly higher than that in the control during germination. The activities of SOD, POD and PAL exhibited similar trends in both groups. The SOD activity in the de-pectinated group was consistently higher than that in the control, while POD and PAL activities, although lower than those in the control, showed a continuous upward trend. The endogenous hormone content (IAA, ABA, GA3, ZR) exhibited fluctuating changes in both groups, with the control group showing a general downward trend, while the de-pectinated group showed a significant increase and a general upward trend. It was preliminarily confirmed that pectin removal could promote the rapid mobilization of stored substances, enzymes and endogenous hormones in the seeds of Taxillus chinensis, promoting the smooth germination of seeds.

Key words: Taxillus chinensis, seed, pectin, germination process, physiological and biochemical indicators

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