芽孢杆菌合成纳米硒的物理化学表征及其应用于富硒番茄栽培

doi:10.14088/j.cnki.issn0439-8114.2026.02.017

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 113-119.doi: 10.14088/j.cnki.issn0439-8114.2026.02.017

芽孢杆菌合成纳米硒的物理化学表征及其应用于富硒番茄栽培

马镝, 盛琪, 阚国仕, 陈红漫

沈阳农业大学生物科学技术学院,沈阳 110866

收稿日期:2025-08-13 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 陈红漫(1969-),女,四川南充人,教授,博士,主要从事微生物生化与代谢工程研究工作,(电话)13840298269(电子信箱)1996500009@syau.edu.cn。
作者简介:马镝(1971-),女,辽宁海城人,副教授,博士,主要从事微生物生化与代谢工程研究工作,(电话)15840082502(电子信箱)2001500028@syau.edu.cn。
基金资助:
辽宁省科技特派行动专项计划项目(2024JH5/10400062)

Physicochemical characterization of nano-selenium synthesized by Bacillus and its application in selenium-enriched tomato cultivation

MA Di, SHENG Qi, KAN Guo-shi, CHEN Hong-man

College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China

Received:2025-08-13 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 从富硒土壤中筛选一株纳米硒合成细菌MC-3,利用形态学观察和16S rDNA序列分析进行菌株鉴定,通过扫描电镜(Scanning electron microscope,SEM)、X射线能谱(Energy dispersive spectrometer,EDS)、X射线衍射(X-ray diffraction,XRD)及傅立叶红外光谱(Fourier transform infrared spectroscopy,FTIR)技术探究其所合成纳米硒(Selenium nanoparticles, SeNPs)的理化特性,并将其应用于富硒番茄栽培试验。结果表明,菌株MC-3为蜡样芽孢杆菌(Bacillus cereus),可还原亚硒酸钠生成球形的非晶态及晶态纳米硒颗粒,粒径范围在100~300 nm。SeNPs主要含C、O、Se等元素,同时含有-OH、C=O和C-O等官能团。将SeNPs作为叶面肥应用于富硒番茄栽培,当SeNPs浓度为70 mg/L时,番茄果实中硒含量可达0.090 mg/kg FW,同时,果实中可溶性蛋白质和维生素C含量分别较CK提高 41.25%和35.00%。以上研究表明,适宜浓度的SeNPs作为叶面肥喷施可用于富硒番茄栽培,并可改善番茄果实品质。

关键词: 芽孢杆菌(Bacillus cereus), 纳米硒, 物理化学表征, 富硒番茄

Abstract: A SeNPs-synthesizing Bacterium MC-3 was isolated from serenium-rich soil and identified through morphological observation and 16S rDNA sequencing analysis. The physicochemical characteristics of SeNPs synthesized by strain MC-3 were analyzed by scanning electron microscope(SEM), energy dispersive spectrometer(EDS), X-ray diffraction(XRD), and Fourier transform infrared spectroscopy(FTIR), and SeNPs were applied to the cultivation experiment of selenium-enriched tomatoes. The results showed that strain MC-3 was Bacillus cereus. The SeNPs from reduction of Na₂SeO₃ by B. cereus MC-3 presented both crystalline and amorphous selenium nanoparticles, with a diameter range of 100~300 nm, and their molecule contain elements such as C, O, and Se, as well as functional groups such as -OH, C=O, and C-O, which were related to the stability of SeNPs. The SeNPs synthesized by B. cereus MC-3 were used as foliar fertilizer in the cultivation of selenium-enriched tomatoes. When the concentration of SeNPs was 70 mg/L, the selenium content in tomato fruits could reach 0.090 mg/kg FW. Meanwhile,the contents of soluble protein and vitamin C in the fruits were 41.25% and 35.00% higher than those in the control group, respectively. In conclusion, the appropriate concentration of SeNPs could be sprayed as foliar fertilizer for selenium-enriched tomato cultivation, and could improve the quality of tomato fruit.

Key words: Bacillus cereus, nano-selenium, physicochemical characterization, selenium-enriched tomato

中图分类号:

Q939.124

马镝, 盛琪, 阚国仕, 陈红漫. 芽孢杆菌合成纳米硒的物理化学表征及其应用于富硒番茄栽培[J]. 湖北农业科学, 2026, 65(2): 113-119.

MA Di, SHENG Qi, KAN Guo-shi, CHEN Hong-man. Physicochemical characterization of nano-selenium synthesized by Bacillus and its application in selenium-enriched tomato cultivation[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 113-119.

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芽孢杆菌合成纳米硒的物理化学表征及其应用于富硒番茄栽培

Physicochemical characterization of nano-selenium synthesized by Bacillus and its application in selenium-enriched tomato cultivation

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