耐硒菌株Bacillus cereus ZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响

doi:10.14088/j.cnki.issn0439-8114.2025.05.005

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (5): 25-32.doi: 10.14088/j.cnki.issn0439-8114.2025.05.005

耐硒菌株Bacillus cereus ZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响

何嘉丽, 刘梦绮, 钟文怡, 何毅, 王璋倩, 程水源

武汉轻工大学硒科学与工程现代产业学院/国家富硒农产品加工技术研发专业中心/湖北省绿色富硒农产品精深加工工程技术研究中心,武汉 430048

收稿日期:2025-02-19 出版日期:2025-05-25 发布日期:2025-06-11
通讯作者: 王璋倩(1984-),女,副教授,博士,主要从事天然产物的研发研究,(电子信箱)wzqsnu@whpu.edu.cn。
作者简介:何嘉丽（1999-）,女,广东湛江人,在读硕士研究生,研究方向为富硒微生物学,（电子信箱）1156181423@qq.com
基金资助:
湖北省自然科学基金项目（2023AFB968）; 湖北省自然科学基金杰出青年项目（2023AFA102）; 湖北省工业微生物重点实验室项目（2024KF07）; 武汉轻工大学科研创新计划项目（2019y05）; 武汉轻工大学硒科学与工程和恩施德源硒材料工程科技有限公司技术储备项目（Se2-202301)

Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain

HE Jia-li, LIU Meng-qi, ZHONG Wen-yi, HE Yi, WANG Zhang-qian, CHENG Shui-yuan

School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University/National R& #x00026;D Center for Se-Rich Agricultural Products Processing/Hubei Engineering and Technical Research Center for Deep Processing of Green Selenium-Rich Agricultural Products, Wuhan 430048, China

Received:2025-02-19 Published:2025-05-25 Online:2025-06-11

摘要/Abstract

摘要： 为更好地利用微生物进行环境生物修复、降低土壤中硒污染和合理利用硒资源等,从湖北省恩施市鱼塘坝富硒土壤中筛选到1株耐硒的菌株ZJ2,经分子生物学鉴定为蜡样芽孢杆菌(Bacillus cereus),该菌株对亚硒酸钠具有较高的耐受能力和还原能力,能将亚硒酸钠还原成稳定的生物纳米硒,并研究了 4种碳源对菌株ZJ2的亚硒酸钠还原能力及其合成纳米硒稳定性的影响。结果表明,菌株ZJ2对5 mmol/L亚硒酸钠还原能力达92%。该菌株合成的生物纳米硒为球形颗粒,平均粒径为（188.42±2.63） nm,Zeta电位为（-41.17±1.77） mV。鼠李糖和葡萄糖的添加对菌株ZJ2还原亚硒酸钠具有显著的促进作用,还原率分别提高37.48个百分点和39.53个百分点,且鼠李糖对菌株合成稳定的纳米硒具有良好的促进作用。通过对ZJ2亚硒酸钠还原能力、纳米硒表征及稳定性等方面的综合评分,可知鼠李糖的添加能明显促进Bacillus cereus ZJ2高效合成更为稳定的生物源纳米硒。

关键词: 耐硒菌株, 蜡样芽孢杆菌(Bacillus cereus), 纳米硒, 还原率, 稳定性, 碳源

Abstract: To better utilize microorganisms for environmental bioremediation, reduce selenium pollution in soil, and rationally exploit selenium resources, a selenium-tolerant strain, ZJ2, was isolated from selenium-rich soil in Yutangba, Enshi City, Hubei Province. Molecular biological identification confirmed it as Bacillus cereus. This strain exhibited high tolerance and reduction capability to sodium selenite (Na₂SeO₃), converting it into stable biogenic selenium nanoparticles (SeNPs). Additionally, the effects of four different carbon sources on the sodium selenite reduction efficiency and the stability of synthesized SeNPs were investigated. The results showed that strain ZJ2 had a 92% reduction capacity to 5 mmol/L sodium selenite. The bio-nanoselenium synthesised by this strain was spherical particles with an average particle size of (188.42±2.63) nm and a Zeta potential of (-41.17±1.77) mV. It was found that the addition of rhamnose and glucose promoted the reduction of sodium selenite by strain ZJ2, with an increase in the reduction rate of 37.48% and 39.53%, respectively. In addition, rhamnose had a good promoting effect on the synthesis of stable selenium nanoparticles by the strain. Based on a comprehensive evaluation of the sodium selenite reduction capability, characterization of selenium nanoparticles (SeNPs) and their stability, it was demonstrated that the addition of rhamnose significantly enhanced the ability of Bacillus cereus ZJ2 to efficiently synthesize more stable biogenic SeNPs.

Key words: selenium-resistant strain, Bacillus cereus, nano-selenium, reduction rate, stability, carbon source

中图分类号:

S154.3

何嘉丽, 刘梦绮, 钟文怡, 何毅, 王璋倩, 程水源. 耐硒菌株Bacillus cereus ZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响[J]. 湖北农业科学, 2025, 64(5): 25-32.

HE Jia-li, LIU Meng-qi, ZHONG Wen-yi, HE Yi, WANG Zhang-qian, CHENG Shui-yuan. Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 25-32.

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耐硒菌株Bacillus cereus ZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响

Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain

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