湖北农业科学 ›› 2019, Vol. 58 ›› Issue (14): 59-61.doi: 10.14088/j.cnki.issn0439-8114.2019.14.014

• 植物保护 • 上一篇    下一篇

西瓜枯萎病病原菌鉴定及药剂联合增效作用

王开成   

  1. 海南省临高县皇桐镇农业服务中心,海南 临高 571823
  • 收稿日期:2019-04-28 出版日期:2019-07-25 发布日期:2019-12-06
  • 作者简介:王开成(1962-),男,海南临高人,农艺师,主要从事农业新技术研究与推广,(电话)13617573390(电子信箱)island62@126.com。

Pathogen identification of watermelon Fusarium oxysporum and its synergistic effect of fungicides

WANG Kai-cheng   

  1. Agricultural Service Center of Huangtong Town of Lingao County,Lingao 571823,Hainan,China
  • Received:2019-04-28 Online:2019-07-25 Published:2019-12-06

摘要: 采用PDA培养基和PD培养基,对西瓜病株病原菌进行分离、培养,经致病性测定,形态学及rDNA-ITS测序,鉴定其致病菌为尖孢镰刀菌(Fusarium oxysporum)。采用菌丝生长速率法测定85%氨基寡糖素和97%咪鲜胺对西瓜尖孢镰孢菌的共毒系数。结果显示,85%氨基寡糖素∶97%咪鲜胺=3∶1时,增效作用最好,共毒系数为167.88,对西瓜枯萎病菌菌丝具有很好的抑制效果。

关键词: 西瓜枯萎病菌(Fusarium oxysporum), 杀菌剂, 联合作用, 混配

Abstract: Pathogen of watermenlon wilt disease was isolated and cultured on PDA and PD, which was indentified was Fusarium oxysporum Schlecht by analysis of morphological characteristics and rDNA-ITS sequence and pathogenic determination. The co-toxicity coefficient of 85% oligosaccharins and 97% prochloraz mixed against Fusarium oxysporum were tested in lab by mycelium growth rate method, respectively. The results showed that synergistic inhibition best when 85% oligosaccharins and 97% prochloraz combinations mixed by 3∶1, co-toxicity coefficient was 167.88, obviously inhibitory against the pathogen.

Key words: Fusarium oxysporum, fungicides, combined action, mixture

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