Cep164基因缺失对小鼠胚胎早期脑发育的影响

doi:10.14088/j.cnki.issn0439-8114.2019.23.033

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 139-141.doi: 10.14088/j.cnki.issn0439-8114.2019.23.033

Cep164基因缺失对小鼠胚胎早期脑发育的影响

王春花

牡丹江师范学院生命科学与技术学院,黑龙江牡丹江 157000

收稿日期:2019-03-20 出版日期:2019-12-10 发布日期:2019-12-18
作者简介:王春花（1984-）,女,黑龙江牡丹江人,讲师,博士,主要从事兽医药理与毒理学、发育生物学研究,（电话）13614631953（电子信箱） swxwch@126.com。
基金资助:
牡丹江师范学院博士启动资金项目（MNUB201407）; 牡丹江师范学院青年创新人才培养项目（QC201601）

Effects of Cep164 gene deletion on embryonic mouse brain development

WANG Chun-hua

School of Life Science and Technology,Mudajiang Normal University,Mudanjiang 157000,Heilongjiang,China

Received:2019-03-20 Online:2019-12-10 Published:2019-12-18

摘要/Abstract

摘要： 利用Foxg1、Gbx2、Otx2、Fgf8和En1探针与小鼠胚胎进行原位杂交,分析Foxg1、Gbx2、Otx2、Fgf8和En1基因在Cep164基因缺失小鼠胚胎脑中的表达情况,确定Cep164基因对脑发育的影响。结果显示,与野生型相比,Cep164基因突变小鼠胚胎头部Gbx2、前中脑Otx2和中后脑的边界Fgf8表达无明显变化,但端脑泡Foxg1表达减少,前神经脊Fgf8表达减少,Otx2在晶状体板和嗅状体板表达减少,背侧中脑和后脑前En1表达减少。这说明Cep164基因是维持胚胎头部正常发育的重要基因,Cep164基因突变会导致前脑发育异常,主要影响嗅觉和视觉功能。

关键词: Cep164, 基因缺失, 小鼠胚胎, 脑发育, 原位杂交

Abstract: To reveal the function of Cep164 gene in mouse brain development, the expression level of Foxg1,Otx2,Gbx2,Fgf8 and En1 in embryonic brain with Cep164 gene deletion was detected by in situ hybridization experiment. The results showed that the expression of Gbx2 and Otx2 in the brain, and Fgf8 in the midbrain-hindbrain boundary, were no significant change in the Cep164 mutation embryonic mice, compared with wild type. But the expression of Foxg1 in telencephalic vesicles, the Fgf8 in anterior neural ridge, the Otx2 in eye and olfaction plate, and En1 in ventral midbrain and anterior hindbrain decreased. The all results reveal Cep164 is an important gene to maintain the normal brain development. Cep164 gene mutation could cause forebrain dysplasia, mainly affect olfaction and vision.

Key words: Cep164, gene deletion, embryonic mouse, brain development, in situ hybridization

中图分类号:

S852.2

王春花. Cep164基因缺失对小鼠胚胎早期脑发育的影响[J]. 湖北农业科学, 2019, 58(23): 139-141.

WANG Chun-hua. Effects of Cep164 gene deletion on embryonic mouse brain development[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(23): 139-141.

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Cep164基因缺失对小鼠胚胎早期脑发育的影响

Effects of Cep164 gene deletion on embryonic mouse brain development

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