UPLC-MS/MS法同时测定中成药和保健食品中非法添加治疗高尿酸血症类药物

doi:10.14088/j.cnki.issn0439-8114.2020.11.026

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (11): 125-130.doi: 10.14088/j.cnki.issn0439-8114.2020.11.026

UPLC-MS/MS法同时测定中成药和保健食品中非法添加治疗高尿酸血症类药物

陈宇, 卢森华, 庞小莲, 徐梦, 丘一仙

玉林市食品药品检验检测中心, 广西玉林 537000

收稿日期:2020-03-31 出版日期:2020-06-10 发布日期:2020-08-06
通讯作者: 卢森华（1985-）, 男, 主管药师, 硕士, 研究方向为食品药品检验与实验室质量管理, （电子信箱）185143773@qq.com。
作者简介:陈宇（1982-）, 男, 广西玉林人, 主管药师, 研究方向为食品药品检验及实验室质量管理, （电话）0775-2380221（电子信箱）48499917@qq.com;
基金资助:
广西壮族自治区玉林市科技计划项目（玉市科201934034）

Determination of hyperuricemia drugs for treatment illegally added in Chinese patent medicines and health foods by UPLC-MS/MS

CHEN Yu, LU Sen-hua, PANG Xiao-lian, XU Meng, QIU Yi-xian

Yulin Center for Food and Drug Control, Yulin 537000, Guangxi, China

Received:2020-03-31 Online:2020-06-10 Published:2020-08-06

摘要/Abstract

摘要： 建立超高效液相色谱-串联质谱法同时检测中成药和保健食品中非法添加的7种治疗高尿酸血症类药物的方法。色谱柱为ACQUITY UPLC® BEH C18（2.1 mm×100 mm, 1.7 μm）, 以0.2%甲酸水溶液-乙腈（流速为 0.3 mL/min）为流动相梯度洗脱;质谱采用电喷雾离子源（ESI）, 正离子多反应监测（MRM）模式进行定性定量分析。结果表明, 7种治疗高尿酸血症类药物在相应的质量浓度范围内线性关系良好, 相关系数均大于0. 995, 在低、中、高3个加标水平下的平均回收率为95.49%~104.87%, RSD为1.40%~3.44%, 检出限为0.01~0.30 μg/kg, 定量限为 0.04~1.00 μg/kg, 6批样品中有1批样品检出了非法添加的化学物质。该方法前处理简单, 灵敏度高, 结果准确, 适用于中成药和保健食品中7种治疗高尿酸血症类药物的同时快速定性定量分析。

关键词: UPLC-MS/MS, 中成药, 保健食品, 非法添加, 高尿酸血症药物

Abstract: To establish a method for simultaneous detection of 7 hyperuricemia drugs for treatment illegally added to Chinese patent medicines and health foods by UPLC-MS/MS．The samples were extracted from samples with mobile phase, the column was a ACQUITY UPLC® BEH C18（2.1 mm×100 mm, 1.7 μm）, a gradient elution with the mobile phase of 0.2% formic acid aqueous solution and acetonitrile solution at a flow rate of 0.3 mL/min. The compounds were detected by electrospray ion source in positive mode with multiple reaction monitoring （MRM） mode．The results showed that all the 7 hyperuricemia drugs for treatment with showed good linearity in their reasonable ranges , and the correlation coefficients were greater than 0.995.The average recoveries of low, medium and high concentrations ranged between 95.49% and 104.87%, and the relative standard deviations were 1.40%~3.44%. The LODs were 0.01~0.30 μg/kg and LOQs were 0.04~1.00 μg/kg.Illegal added chemical drugs were found in 1 of the 6 tested practical samples. With the advantages of simple pre-processed, high sensitivity and accurate results, the method can be used in determination of 7 hyperuricemia drugs for treatment in Chinese patent medicine and health foods.

Key words: UPLC-MS/MS, Chinese patent medicine, health food, illegal addition, hyperuricemia drugs

中图分类号:

O657.7⁺2

陈宇, 卢森华, 庞小莲, 徐梦, 丘一仙. UPLC-MS/MS法同时测定中成药和保健食品中非法添加治疗高尿酸血症类药物[J]. 湖北农业科学, 2020, 59(11): 125-130.

CHEN Yu, LU Sen-hua, PANG Xiao-lian, XU Meng, QIU Yi-xian. Determination of hyperuricemia drugs for treatment illegally added in Chinese patent medicines and health foods by UPLC-MS/MS[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(11): 125-130.

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UPLC-MS/MS法同时测定中成药和保健食品中非法添加治疗高尿酸血症类药物

Determination of hyperuricemia drugs for treatment illegally added in Chinese patent medicines and health foods by UPLC-MS/MS

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