十堰市白菜黑斑病病原真菌的分离与鉴定

doi:10.14088/j.cnki.issn0439-8114.2024.10.010

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (10): 59-63.doi: 10.14088/j.cnki.issn0439-8114.2024.10.010

十堰市白菜黑斑病病原真菌的分离与鉴定

石宪铭^1a,1b, 何亚慧², 胡燕萍³, 吴飞扬^1a,1b, 彭晓璇³, 张勇洪^1a,1b, 游磊^1a,1b, 温毅华³

1.湖北医药学院,a.基础医学院;b.生物医药研究院/武当特色中药研究湖北省重点实验室与工程技术中心,湖北十堰 442000;
2.十堰市农业科学院,湖北十堰 442000;
3.十堰市蔬菜科学研究所,湖北十堰 442000

收稿日期:2023-05-22 出版日期:2024-10-25 发布日期:2024-11-05
通讯作者: 温毅华（1966-）,男,湖北天门人,高级农艺师,主要从事蔬菜栽培与遗传育种研究,（电话）0719-8117598（电子信箱）447656967@qq.com;游磊（1989-）,男,湖北潜江人,副教授,主要从事药用植物学与表观遗传药理学研究,（电子信箱）youlei@hbmu.edu.cn。
作者简介:石宪铭（1996-）,男,江苏宿迁人,在读硕士研究生,研究方向为病理学与病理生理学,（电子信箱）18360167144@163.com;并列第一作者,何亚慧（1981-）,女（蒙古族）,内蒙古宁城人,农艺师,博士,主要从事农作物栽培技术研究,（电子信箱）179117170@qq.com。
基金资助:
国家自然科学基金项目（32200680）; 湖北省教育厅项目（JYT2021009）

Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City

SHI Xian-ming^1a,1b, HE Ya-hui², HU Yan-ping³, WU Fei-yang^1a,1b, PENG Xiao-xuan³, ZHANG Yong-hong^1a,1b, YOU Lei^1a,1b, WEN Yi-hua³

1a. School of Basic Medical Sciences;1b. Biomedical Research Institute/Hubei Key Laboratory, Engineering Technology Center of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan 442000, Hubei, China;
2. Shiyan Academy of Agricultural Sciences, Shiyan 442000, Hubei, China;
3. Shiyan Vegetable Science Research Institute, Shiyan 442000, Hubei, China

Received:2023-05-22 Published:2024-10-25 Online:2024-11-05

摘要/Abstract

摘要： 对湖北省十堰市当地白菜（Brassica pekinensis）品种的黑斑病进行病原真菌鉴定和病害分析,并提出相应的预防措施。采用组织分离法从病叶中分离得到病原真菌,并利用rDNA ITS和Alt-α1基因序列比对进行分析鉴定。结果表明,发现1个新的链格孢属（Alternaria sp.）真菌,推测其是导致十堰市当地白菜黑斑病的致病真菌。此外,确认ITS序列和Alt-α1基因序列的联合分析,能够区分3种常见的链格孢属真菌Alternaria brassicicola、Alternaria japonica、Alternaria brassicae。

关键词: 白菜（Brassica pekinensis）, rDNA ITS, 黑斑病, 病原真菌, 分离与鉴定, 湖北省十堰市

Abstract: The pathogenic fungi of the black spot of Brassica pekinensis in Shiyan City, Hubei Province were identified and analyzed, and the corresponding preventive measures were proposed. The pathogenic fungi were isolated from diseased leaves by tissue separation and identified by rDNA ITS and Alt-α 1 gene sequence alignment. The results showed that a new Alternaria sp. fungus was found, which was presumed to be the pathogenic fungus causing the local cabbage black spot disease in Shiyan City. In addition, it was confirmed that the joint analysis of ITS sequence and Alt-α 1 gene sequence could distinguish three common Alternaria fungi： Alternaria Brassicola, Alternaria japonica and Alternaria brassicae.

Key words: Chinese cabbage（Brassica pekinensis）, rDNA ITS, black leaf spot disease, pathogenic fungi, separation and identification, Shiyan City, Hubei Province

中图分类号:

S436.341

石宪铭, 何亚慧, 胡燕萍, 吴飞扬, 彭晓璇, 张勇洪, 游磊, 温毅华. 十堰市白菜黑斑病病原真菌的分离与鉴定[J]. 湖北农业科学, 2024, 63(10): 59-63.

SHI Xian-ming, HE Ya-hui, HU Yan-ping, WU Fei-yang, PENG Xiao-xuan, ZHANG Yong-hong, YOU Lei, WEN Yi-hua. Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(10): 59-63.

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十堰市白菜黑斑病病原真菌的分离与鉴定

Separation and identification of pathogenic fungi causing Chinese cabbage black leaf spot disease in Shiyan City

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