湖北农业科学 ›› 2019, Vol. 58 ›› Issue (18): 55-57.doi: 10.14088/j.cnki.issn0439-8114.2019.18.013

• 植物保护 • 上一篇    下一篇

宜昌市一种核桃黑斑病病原菌的分离及鉴定

王鹏程1, 吴楚2, 孙智1, 彭新3, 韩永世4, 孙长城5, 贾切2   

  1. 1.湖北三峡职业技术学院,湖北 宜昌 443000;
    2.长江大学园艺园林学院,湖北 荆州 434000;
    3.秭归县郭家坝镇林业站,湖北 秭归 443621;
    4.秭归县林业局,湖北 秭归 443600;
    5.兴山县峡口镇农技服务中心,湖北 兴山 443701
  • 收稿日期:2019-04-15 出版日期:2019-09-25 发布日期:2019-11-08
  • 通讯作者: 贾 切(1980-),男,讲师,博士,主要从事园林园艺植物保护的教学与研究工作,(电话)15571693769,(电子信箱)jiaqie020@163.com。
  • 作者简介:王鹏程(1968-),男,湖北谷城人,副教授,硕士,主要从事园林园艺的教学与研究工作,(电话)15271519816,(电子信箱) wpc061227@126.com

Isolation and identification of a pathogen of walnut black spot in Yichang city

WANG Peng-cheng1, WU Chu2, SUN Zhi1, PENG Xin3, HAN Yong-shi4, SUN Chang-cheng5, JIA Qie2   

  1. 1.Hubei Three Gorges Polytechnic, Yichang 443000,Hubei,China;
    2.College of Horticulture and Gardening,Yangtze University,Jingzhou 434000,Hubei,China;
    3.Guojiaba Town Forestry Station of Zigui County,Zigui 443621,Hubei,China;
    4.Zigui County Forestry Bureau,Zigui 443600,Hubei,China;
    5.Xiakou Town Agricultural Technology Service Center of Xingshan County,Xingshan 443701,Hubei,China
  • Received:2019-04-15 Online:2019-09-25 Published:2019-11-08

摘要: 为明确引起宜昌市核桃黑斑病的病原菌,根据柯赫氏法则测定其致病性,通过形态学观察和rDNA-ITS序列分析鉴定病原菌。结果表明,引起宜昌市核桃黑斑病的病原菌为链格孢属链格孢(Alternaria alternata)。

关键词: 核桃黑斑病, 分离鉴定, 形态学观察, 链格孢(Alternaria alternata), 宜昌市

Abstract: To identify the pathogen causing walnut black spot in Yichang city, the pathogenicity was determined according to Koch's rule. And the pathogenic bacteria was identified by morphological characteristics and rDNA-ITS sequence analysis. The results showed that Alternaria altenata was the pathogen causing walnut black spot in Yichang city.

Key words: walnut black spot, isolation and identification, morphological observation, Alternaria alternata, Yichang city

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