湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 142-144.doi: 10.14088/j.cnki.issn0439-8114.2019.23.034

• 畜牧·兽医 • 上一篇    下一篇

仔猪黄痢大肠杆菌的分离鉴定

刘泽文, 袁芳艳, 刘威, 高婷, 周丹娜, 杨克礼, 段正赢, 郭锐, 梁婉, 田永祥   

  1. 湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室,武汉 430064
  • 收稿日期:2019-09-15 出版日期:2019-12-10 发布日期:2019-12-18
  • 通讯作者: 田永祥(1965-),男,研究员,主要从事畜禽传染病研究工作,(电子信箱)tyxanbit@163.com。
  • 作者简介:刘泽文(1969-),男,湖北赤壁人,副研究员,主要从事家畜疾病诊断与预防研究工作,(电话)13986152053(电子信箱)liuzwen2004@sina.com
  • 基金资助:
    国家重点研发计划项目(2017YFD0500600); 湖北省农业科技创新中心项目(2016-620-000-001-026); 湖北省技术创新专项重大项目(2016ABA124)

Isolation and identification of Escherichia coli from yellow diarrhea in piglets

LIU Ze-wen, YUAN Fang-yan, LIU Wei, GAO Ting, ZHOU Dan-na, YANG Ke-li, DUAN Zheng-ying, GUO Rui, LIANG Wan, TIAN Yong-xiang   

  1. Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Science/Key Laboratory of Prevention and Control Agents for Animal Bacteriocins,Ministry of Agriculture and Aural Affairs,Wuhan 430064,China
  • Received:2019-09-15 Online:2019-12-10 Published:2019-12-18

摘要: 湖北省红安县某猪场新生仔猪发生一种以剧烈腹泻、排出黄色粪便并迅速脱水死亡为主要症状的疾病,经病原分离、革兰氏染色、PCR鉴定、生化试验和血清型鉴定,确定新生仔猪病是由大肠杆菌感染引起。经血清型鉴定,该分离菌株属于纤毛抗原987P型,该菌株对头孢曲松、头孢噻肟较敏感。

关键词: 仔猪黄痢, 大肠杆菌, 分离鉴定

Abstract: In a pig farm in Hong'an county, Hubei province, newborn piglets developed a disease characterized by severe diarrhea, discharge of yellow feces and rapid dehydration; after pathogen isolation, Gram staining, PCR identification, biochemical tests and serotype identification, E. coli infection in newborn piglets was determined. According to the serotype identification, the isolated strain belongs to the cilia antigen 987P type, and the strain is sensitive to ceftriaxone and cefotaxime.

Key words: piglet scutellaria, Escherichia coli, isolation and identification

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