八种肺衣属地衣内生真菌的鉴定及抗菌活性研究

doi:10.14088/j.cnki.issn0439-8114.2019.12.021

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (12): 92-96.doi: 10.14088/j.cnki.issn0439-8114.2019.12.021

八种肺衣属地衣内生真菌的鉴定及抗菌活性研究

范海云, 滕梦婷, 周生亮, 马琳, 李鹰, 刘欣欣, 丁孺孺, 张鹏

江苏师范大学生命科学学院,江苏徐州 221116

收稿日期:2018-11-21 出版日期:2019-06-25 发布日期:2019-12-05
通讯作者: 张鹏,男,副教授,主要从事创新药物的研究与开发,（电子信箱）zhangpeng@jsnu.edu.cn。
作者简介:范海云（1995-）,女,河北张家口人,在读本科生,（电话）18252161076（电子信箱）2074968664@qq.com。
基金资助:
国家自然科学基金项目（31300067）; 江苏省大学生实践创新训练计划项目（201710320107X）

Identification and study of eight endophytic fungi with antimicrobial activity from Lobaria sp.

FAN Hai-yun, TENG Meng-ting, ZHOU Sheng-liang, MA Lin, LI Ying, LIU Xin-xin, DING Ru-ru, ZHANG Peng

College of Life Sciences,Jiangsu Normal University,Xuzhou 221116,Jiangsu,China

Received:2018-11-21 Online:2019-06-25 Published:2019-12-05

摘要/Abstract

摘要： 采用平板分离法,从四川省黄龙、九寨沟等地采集肺衣属（lobaria sp.）地衣中分离内生真菌,分别对其进行液体发酵后检测发酵液的抑菌活性,并对具有抑菌活性的内生真菌进行形态学及分子生物学鉴定。结果表明,分离得到37株内生真菌,其中有8株内生真菌的发酵液具有一定的抑菌活性,分别属于黑孢属、链格孢属、镰刀菌属和木霉属等。

关键词: 肺衣属（lobaria sp.）地衣, 内生真菌, 分离鉴定, 抗菌活性

Abstract: Using plate separation method, and endophytic fungi was isolated from Lobaria sp. lichens, which were collected from Huanglong and Jiuzhaigou of Sichuan province. These endophytic fungi were inoculated into liquid medium. The antimicrobial activity of the fermentation fluid were respectively evaluated. These fungi were identified by morphology and molecular biology method. The results showed that 37 endophytic fungi were isolated, and 8 of which had antibacterial activity, which belonged to Melanspora, Streptospora, Fusarium and Trichoderma, respectively.

Key words: Lobaria sp. lichen, endophytic fungi, separation and identification, antibacterial activity

中图分类号:

Q949.34
Q93

范海云, 滕梦婷, 周生亮, 马琳, 李鹰, 刘欣欣, 丁孺孺, 张鹏. 八种肺衣属地衣内生真菌的鉴定及抗菌活性研究[J]. 湖北农业科学, 2019, 58(12): 92-96.

FAN Hai-yun, TENG Meng-ting, ZHOU Sheng-liang, MA Lin, LI Ying, LIU Xin-xin, DING Ru-ru, ZHANG Peng. Identification and study of eight endophytic fungi with antimicrobial activity from Lobaria sp.[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 92-96.

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八种肺衣属地衣内生真菌的鉴定及抗菌活性研究

Identification and study of eight endophytic fungi with antimicrobial activity from Lobaria sp.

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