湖北农业科学 ›› 2025, Vol. 64 ›› Issue (8): 54-61.doi: 10.14088/j.cnki.issn0439-8114.2025.08.009

• 育种·栽培 • 上一篇    下一篇

测交鉴定结合数量性状遗传分析创制糯高粱保持系资源

韩永亮1, 方路斌1, 罗河月1, 崔江慧2, 郭群3, 常金华2   

  1. 1.邯郸市农业科学院资源与环境研究所,河北 邯郸 056000;
    2.河北农业大学农学院,河北 保定 071001;
    3.河北汉青种业科技有限公司,河北 沧州 061000
  • 收稿日期:2025-05-28 出版日期:2025-08-25 发布日期:2025-09-12
  • 通讯作者: 方路斌,副研究员,主要从事作物遗传育种研究,(电子信箱)hdnkyflb @163.com。
  • 作者简介:韩永亮(1979-),男,河北磁县人,副研究员,主要从事作物遗传育种和配套栽培技术研究,(电子信箱)13785032797@126.com;
  • 基金资助:
    河北省农业科技成果转化项目; 2025年邯郸市农业科学院自主创新项目

The creation of waxy sorghum maintainer line resources by test cross identification combined with quantitative trait genetic analysis

HAN Yong-liang1, FANG Lu-bin1, LUO He-yue1, CUI Jiang-hui2, GUO Qun3, CHANG Jin-hua2   

  1. 1. Institute of Resources and Environment, Handan Academy of Agricultural Sciences, Handan 056000, Hebei, China;
    2. College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China;
    3. Hebei Hanqing Seed Technology Co., Ltd., Cangzhou 061000, Hebei, China
  • Received:2025-05-28 Published:2025-08-25 Online:2025-09-12

摘要: 生产上三系杂交糯高粱的应用一直因优良糯性保持系种质资源缺乏而受到制约,为了快速创制高粱糯性保持系种质资源,以矮脚糯高粱为父本、保持系材料TX623B为母本,构建F2分离群体及F2:3家系群体,采用主基因+多基因混合模型分析高粱数量性状株高和茎粗的遗传规律。结果表明,株高受2对主基因控制,遗传力较高;茎粗受1对主基因控制,遗传力偏低。基于遗传分析结果,采用DPS数据处理系统建立了基于模糊相似优先比的加权综合评价筛选体系,并对F2:3家系进行了加权综合评价,通过综合评价对优良家系材料在品种选育中的应用方向进行了评估,筛选出综合性状良好且表型性状接近TX623B的糯保持系材料,为高粱品种选育与杂种优势利用工作提供了方法依据和材料基础。

关键词: 糯高粱, 保持系, 回交转育, 测交, 数量性状, 株高, 茎粗, 主基因+多基因混合遗传模型

Abstract: The application of three-line hybrid waxy sorghum in production had been constrained by the scarcity of elite waxy maintainer germplasm resources. To rapidly create waxy sorghum maintainer germplasm resources, the F2 segregating population and F2:3 family population were constructed by using Aijiaonuo as the male parent and maintainer line TX623B as the female parent. The inheritance of sorghum quantitative traits such as plant height and stem diameter was analyzed by using the major gene+polygene mixed model. The results showed that the plant height was controlled by two pairs of major genes, and the heritability was high. Stem diameter was controlled by a pair of major genes, and the heritability was low. Based on the results of genetic analysis, a weighted comprehensive evaluation and screening system based on fuzzy similarity priority ratio was established by DPS data processing system, and the weighted comprehensive evaluation of F2:3 families was carried out. The application direction of excellent family materials in variety breeding was evaluated by comprehensive evaluation, and waxy maintainer materials with good comprehensive traits and phenotypic traits close to TX623B were screened out, which provided a method basis and material basis for sorghum variety breeding and heterosis utilization.

Key words: waxy sorghum, maintainer line, backcross breeding, test cross, quantitative trait, plant height, stem diameter, major gene + polygene mixed genetic model

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