广西不同养殖模式猪源大肠杆菌耐药表型差异性分析

doi:10.14088/j.cnki.issn0439-8114.2020.01.025

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (1): 114-118.doi: 10.14088/j.cnki.issn0439-8114.2020.01.025

广西不同养殖模式猪源大肠杆菌耐药表型差异性分析

冯世文, 李军, 潘艳, 贺会利, 彭昊, 钟舒红, 胡帅, 杨威

广西兽医研究所/广西兽医生物技术重点实验室,南宁 530001

收稿日期:2019-01-24 出版日期:2020-01-10 发布日期:2020-01-10
通讯作者: 李军（1971-）,男（汉族）,副研究员,博士,主要从事动物疫病防控与病原分子生物学研究,（电子信箱）jlee9981@163.com。
作者简介:冯世文（1988-）,男（壮族）,广西大新人,在读博士研究生,研究方向为兽医药理学,（电话）15078764005（电子信箱）fengshw1205@163.com
基金资助:
广西科技重大专项（桂科AA17204057）; 广西科技基地和人才专项（桂科AD17195015）; 广西水产畜牧兽医局科技项目（桂渔牧科201528005）

Analysis on drug resistance phenotype of Escherichia coli isolated from different pigs cultivation modes in Guangxi

FENG Shi-wen, LI Jun, PAN Yan, HE Hui-li, PENG Hao, ZHONG Shu-hong, HU Shuai, YANG Wei

Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology,Nanning 530001,China

Received:2019-01-24 Online:2020-01-10 Published:2020-01-10

摘要/Abstract

摘要： 为调查广西不同养殖模式的猪源大肠杆菌耐药表型差异,为合理使用抗菌药物防治猪大肠杆菌病和减缓耐药菌株产生提供参考,采用体外分离培养和体外药敏试验的方法,分离了广西不同养殖模式的猪源大肠杆菌,并进行耐药表型检测和差异性分析。结果显示,大规模养猪场、中小规模养猪场和散养户的猪源大肠杆菌对头孢西丁、头孢他啶和阿米卡星的敏感率高于70%,而对青霉素G、氨苄西林、阿莫西林、利福平、林可霉素和复方新诺明的耐药率均高于90%。大规模养猪场大肠杆菌对恩诺沙星的耐药率极显著低于散养户（P<0.01）,对氧氟沙星、庆大霉素和氟苯尼考的耐药率显著低于散养户（P<0.05）;中小规模养猪场大肠杆菌对恩诺沙星、强力霉素和氟苯尼考的耐药性显著低于散养户（P<0.05）;散养户大肠杆菌对环丙沙星、卡那霉素和壮观霉素的耐药率极显著高于大规模养猪场和中小规模养猪场（P<0.01）,而对头孢噻肟的耐药率极显著低于大规模养猪场和中小规模养猪场（P<0.01）。大规模养殖场、中小规模养殖场和散养户大肠杆菌的多重耐药指数（MARI）分别为0.62、0.63和0.68。14~23耐,散养户（44株）极显著高于大规模养猪场（30株）（P<0.01）,显著高于中小规模养猪场（39株）（P<0.05）。结果表明,3种养殖模式的猪源大肠杆菌的耐药情况不同,但均存在严重的耐药问题,且以多重耐药为主。

关键词: 养殖模式, 猪源致病性大肠杆菌, 耐药性, 广西

Abstract: This study was to investigate the difference of drug resistance phenotype of E. coli isolated from different pigs cultivation modes in guangxi, and to provide a reference for the scientific use of antimicrobial drugs of swine colibacillosis and to slow down the drug resistant strains. E. coli from different pigs cultivation modes in Guangxi was isolated and identified, and the drug-resistant phenotype was tested and analysis. The results showed that, the E.coli isolates from three pigs cultivation modes were highly sensitive to cefoxitin, ceftazidime and amikacin, the sensitive rate up to 70%, while highly resistant to penicillin G, ampicillin, amoxicillin, rifampicin, lincomycin and sulfamethoxazole, the resistance rate up to 90%. The resistance rate of E.coli from large scale pig farms against enrofloxacin was extremely significantly lower than free-range farming （P<0.01）, the resistance rate of E.coli from large scale pig farms against ofloxacin, gentamicin, florfenicol were significantly lower than free-range farmers （P<0.05）. The resistance rate of E. coli from small and medium-sized pig farms against enrofloxacin, doxycycline and florfenicol were significantly lower than free-range farmers （P<0.05）. The resistance rate of E. coli from free-range farmers against ciprofloxacin, kanamycin, spectinomycin were extremely significantly higher than large scale pig farms and small and medium-sized pig farms（P<0.01）, while the resistance rate against cefotaxime was extremely significantly lower than large scale pig farms and small and medium-sized pig farms（P<0.01）. The multiple antibiotics resistance index （MARI） of E. coli from large scale pig farms, small and medium-sized pig farms and free-range farmers was 0.62, 0.63 and 0.68. 14 to 23 resistant E. coli strains accounted in free-range farming, which was extremely significantly higher than large scale pig farms（P<0.01）, and was significantly higher than small and medium-sized pig farms（P<0.05）. The results indicated that the resistance of E. coli from different pigs cultivation mode were different but all very serious and mainly of multi-drug resistance.

Key words: cultivation mode, pig Escherichia coli, drug-resistant, Guangxi

中图分类号:

S858.28

冯世文, 李军, 潘艳, 贺会利, 彭昊, 钟舒红, 胡帅, 杨威. 广西不同养殖模式猪源大肠杆菌耐药表型差异性分析[J]. 湖北农业科学, 2020, 59(1): 114-118.

FENG Shi-wen, LI Jun, PAN Yan, HE Hui-li, PENG Hao, ZHONG Shu-hong, HU Shuai, YANG Wei. Analysis on drug resistance phenotype of Escherichia coli isolated from different pigs cultivation modes in Guangxi[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 114-118.

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广西不同养殖模式猪源大肠杆菌耐药表型差异性分析

Analysis on drug resistance phenotype of Escherichia coli isolated from different pigs cultivation modes in Guangxi

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