湖北农业科学 ›› 2021, Vol. 60 ›› Issue (10): 67-71.doi: 10.14088/j.cnki.issn0439-8114.2021.10.013

• 植物保护 • 上一篇    下一篇

L-谷氨酸-拌种灵耦合物的合成、生物活性及在烟草植株内的传导性

常悦1, 李俊凯1, 陈顺顺1, 徐汉虹2   

  1. 1. 长江大学农学院,湖北 荆州 434025;
    2. 华南农业大学植物保护学院,广州 510642
  • 收稿日期:2021-01-27 出版日期:2021-05-25 发布日期:2021-05-28
  • 通讯作者: 李俊凯(1969-),男,教授,博士,主要从事新农药的研究与开发,(电子信箱)junkaili@sina.com。
  • 作者简介:常悦(1995-),男,山西朔州人,在读硕士研究生,研究方向为新农药的研究与开发,(电话)15629867590(电子信箱)2712076820@qq.com。
  • 基金资助:
    国家自然科学基金项目(31672069)

Synthesis,biological activity and translocation of L-Glu-amicarthiazol conjugate in tobacco plants

CHANG Yue1, LI Jun-kai1, CHEN Shun-shun1, XU Han-hong2   

  1. 1. College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;
    2. College of Plant Protection,South China Agricultural University,Guangzhou 510642,China
  • Received:2021-01-27 Online:2021-05-25 Published:2021-05-28

摘要: 以L-谷氨酸和拌种灵为原料,合成了L-谷氨酸-拌种灵耦合物,并通过1H NMR、13C NMR和质谱表征其结构。采用菌丝生长速率法测定供试化合物对水稻纹枯病菌(Rhizoctonia solani)的室内毒力。采用高效液相色谱法,通过叶片涂布和水培2种处理方式检测了供试化合物在烟草植株上的传导性。结果表明,L-谷氨酸-拌种灵耦合物与拌种灵相比,一定程度上保留了对水稻纹枯病菌抑制活性,其对水稻纹枯病菌的EC50为13.76 μmol/L(拌种灵对水稻纹枯病的EC50为12.47 μmol/L)。烟草植株对L-谷氨酸-拌种灵耦合物的吸收、传导量与对照药剂拌种灵相比显著增加。

关键词: 氨基酸, 拌种灵, 耦合物, 生物活性, 传导性

Abstract: L-Glu-amicarthiazol conjugate was synthesized using L-glutamic acid and amicarthiazol as raw materials, and its structure was characterized by 1 H NMR, 13C NMR and mass spectrometry. The indoor toxicity of compounds against rice sheath blight were evaluated by the mycelial growth rate method. High performance liquid chromatography (HPLC) was used to detect the translocation of compounds on tobacco plants through leaf coating and hydroponic treatment. The results showed that compared with amicarthiazol, L-Glu-amicarthiazol conjugate retained the inhibitory activity against rice sheath blight to a certain extent, and its EC50 against rice sheath blight was 13.76 μmol/L (The EC50 of amicarthiazol against rice sheath blight was 12.47 μmol/L). The uptake and translocation of L-Glu-amicarthiazol conjugate in tobacco was significantly increased compared with that of the amicarthiazol.

Key words: amino acid, amicarthiazol, conjugate, biological activity, translocation

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