长雄野生稻与栽培稻种间杂种胚拯救研究初报

doi:10.14088/j.cnki.issn0439-8114.2021.21.006

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (21): 30-32.doi: 10.14088/j.cnki.issn0439-8114.2021.21.006

长雄野生稻与栽培稻种间杂种胚拯救研究初报

査中萍, 郑兴飞, 周黎, 王红波, 殷得所, 胡建林, 董华林, 游艾青, 徐得泽

湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉 430064

收稿日期:2021-02-20 出版日期:2021-11-10 发布日期:2021-11-24
通讯作者: 徐得泽（1978-）,男,安徽庐江人,副研究员,硕士,主要从事优质专用水稻研究,（电子信箱）dezexu@163.com。
作者简介:査中萍（1971-）,女,安徽安庆人,副研究员,硕士,主要从事水稻遗传育种研究,（电话）13986019016（电子信箱）zhongpingzha@163.com。
基金资助:
湖北省农业科学院青年科学基金项目（2020NKYJJ01）; 湖北省粮食作物种质创新与遗传改良重点实验室开放课题（2018lzjj03）; 湖北省重点研发计划项目（2020BBB053）

Preliminary reports on embryo rescue of interspecific hybrid between Oryza longistaminata and cultivated rice

ZHA Zhong-ping, ZHENG Xing-fei, ZHOU Li, WANG Hong-bo, YIN De-suo, HU Jian-lin, DONG Hua-lin, YOU Ai-qing, XU De-ze

Food Crops Institute,Hubei Academy of Arricultural Sciences/Hubei Key laouatory of Food Crop Germplasm and Genetic Improvement,Wuhan 430064,China

Received:2021-02-20 Online:2021-11-10 Published:2021-11-24

摘要/Abstract

摘要： 以优良栽培稻恢复系为母本,与长雄野生稻杂交,在授粉后第7~9 天对杂交后的幼胚进行离体培养。一共接种幼胚450枚,获得发育良好健壮的绿苗438株,胚拯救效率为97.3%。20 d后待组培苗约8~10 cm时,通过室内炼苗再将幼苗移入育秧盘中,10 d后统计移栽的438株幼苗,共存活434株,成活率达99.1%。

关键词: 野生稻, 栽培稻, 中间杂种, 胚拯救

Abstract: Hybrid fine cultivated rice restorer lines as female parent with Oryza longistaminata, young hybridized embryos 7~9 days after pollination were cultured in vitro. A total of 450 young embryos were inoculated, and a total of 438 well-developed and robust green seedlings were obtained. The embryo rescue efficiency was 97.3%. After 20 days, when the tissue culture seedlings were about 8~10 cm, the seedlings were transferred to the seedling tray through indoor hardening.10 days later, 438 seedlings were transplanted, and 434 survived with a survival rate of 99.1%.

Key words: wild rice （Oryza longistaminata）, cultivated rice, interspecific hybrid, embryo rescue

中图分类号:

S330
S511.5

査中萍, 郑兴飞, 周黎, 王红波, 殷得所, 胡建林, 董华林, 游艾青, 徐得泽. 长雄野生稻与栽培稻种间杂种胚拯救研究初报[J]. 湖北农业科学, 2021, 60(21): 30-32.

ZHA Zhong-ping, ZHENG Xing-fei, ZHOU Li, WANG Hong-bo, YIN De-suo, HU Jian-lin, DONG Hua-lin, YOU Ai-qing, XU De-ze. Preliminary reports on embryo rescue of interspecific hybrid between Oryza longistaminata and cultivated rice[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(21): 30-32.

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长雄野生稻与栽培稻种间杂种胚拯救研究初报

Preliminary reports on embryo rescue of interspecific hybrid between Oryza longistaminata and cultivated rice

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