PpPRR5基因在不同光合速率梨品种中的表达分析

doi:10.14088/j.cnki.issn0439-8114.2021.23.037

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (23): 168-171.doi: 10.14088/j.cnki.issn0439-8114.2021.23.037

PpPRR5基因在不同光合速率梨品种中的表达分析

刘政¹, 安琳², 伍涛¹, 秦仲麒¹, 杨立¹, 程寅胜¹

1.湖北省农业科学院果树茶叶研究所/湖北省农业科技创新中心果树茶叶研究分中心,武汉 430064;
2.华中农业大学园艺植物生物学教育部重点实验室,武汉 430072

收稿日期:2021-08-01 出版日期:2021-12-10 发布日期:2021-12-21
通讯作者: 秦仲麒,研究员,主要从事梨树育种、栽培及分子生物学研究,（电话）027-87770251（电子信箱）zhongqiqin@163.com。
作者简介:刘政（1986-）,男,湖北武汉人,副研究员,博士,主要从事梨树栽培生理及分子生物学研究,（电话）15802782989（电子信箱）liuzhenghzau@aliyun.com;
基金资助:
国家重点研发计划资助项目（2020YFD1000202; 2018YFD1000200）; 国家现代农业产业技术体系建设专项（CARS-29-17）; 湖北省农业科技创新中心重大研发项目（2020-620-000-002-05）; 国家自然科学基金项目（31801819）; 湖北省农业科技创新中心资助项目（2019-620-000-001-22）

Expression analysis of PpPRR5 gene in pear genotypes with different degrees of photosynthetic rate

LIU Zheng¹, AN Lin², WU Tao¹, QIN Zhong-qi¹, YANG Li¹, CHENG Yin-sheng¹

1. Institute of Fruit and Tea, Hubei Academy of Agricultural Science/Fruit and Tea Research Branch, Hubei Innovation Center of Agricultural Science and Technology,Wuhan 430064,China;
2. Key Laboratory of Horticultural Plant Biology （Ministry of Education）,Huazhong Agricultural University,Wuhan 430072,China

Received:2021-08-01 Online:2021-12-10 Published:2021-12-21

摘要/Abstract

摘要： 提高光合作用水平是提高果树产量潜力的有效途径。生物钟基因能够响应外界环境信号,并在光合作用中发挥调控作用。研究分析了不同类型梨品种（包括砂梨、白梨以及西洋梨）光合作用差异,检测了PpPRR5基因在不同品种中的表达水平变化模式。结果表明,不同梨品种的净光合速率存在显著差异,砂梨品种翠冠净光合速率最高,白梨品种居中,西洋梨品种阿巴特净光合速率最低。表达分析发现,与梨树光合作用密切相关的生物钟基因PpPRR5表达水平与不同品种光合作用能力呈负相关性,表明PpPRR5可能是梨树光合作用的负调控因子。

关键词: 梨树, 光合作用, 不同品种, PpPRR5, 表达水平

Abstract: Improvement in photosynthesis is an effective way to further increase yield potential in fruit crops. Circadian clock genes could be adjusted by environmental cues, and thereby regulate photosynthesis. The differences of photosynthesis of different types of pear varieties （including Pyrus pyrifolia, Pyrus bretschneider and western pear） were studied and analyzed, and the expression level and change pattern of PpPRR5 gene in different varieties were detected. The results showed that significantly different levels of net photosynthetic rate were observed in five pear genotypes. Pyrus pyrifolia Nakaicv. ‘Cuiguan' had the highest levels of net photosynthetic rate. Pyrus bretschneider maintained moderate levels of net photosynthetic rate. Pyrus communis L. cv. ‘Abate Fetel' showed the lowest levels of net photosynthetic rate. There was a negative correlation between the expression of circadian clock gene PpPRR5 and the capacity of pear genotype for photosynthetic rate. The result suggests that PpPRR5 acts as a negative regulator of photosynthetic performance.

Key words: pear, photosynthesis, different genotypes, PpPRR5, expression levels

中图分类号:

Q819
S661.2

刘政, 安琳, 伍涛, 秦仲麒, 杨立, 程寅胜. PpPRR5基因在不同光合速率梨品种中的表达分析[J]. 湖北农业科学, 2021, 60(23): 168-171.

LIU Zheng, AN Lin, WU Tao, QIN Zhong-qi, YANG Li, CHENG Yin-sheng. Expression analysis of PpPRR5 gene in pear genotypes with different degrees of photosynthetic rate[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 168-171.

参考文献 23

[1]	INOUE K, ARAKI T, ENDO M.Oscillator networks with tissue-specific circadian clocks in plants[J]. Sem Cell Dev Biol, 2018, 83:78-85.
[2]	CANO-RAMIREZ D, DODD A N.New connections between circadian rhythms, photosynthesis, and environmental adaptation[J]. Plant cell environ, 2018, 41:2515-2517.
[3]	NOHALES M A,KAY S A.Molecular mechanisms at the core of the plant circadian oscillator[J]. Nat Struct Mol Biol,2016,23:1061-1069.
[4]	ZHANG Y,PFEIFFER A,TEPPERMAN J M,et al. Central clock components modulate plant shade avoidance by directly repressing transcriptional activation activity of PIF proteins[J]. Proc natlacadsci USA,2020,117:3261-3269.https://doi.org/10.1073/pnas.1918317 117.
[5]	HENRIQUES R, RAPDI C, AHMAD Z, et al.Circadian regulation of plant growth[J]. Annuplant reviews, 2018, 1:1-29.
[6]	SRIVASTAVA D, SHAMIM M, KUMAR M, et al.Role of circadian rhythm in plant system: An update from development to stress response[J]. Environ Exp Bot, 2019, 162:256-271.
[7]	RESCO DE DIOS V, LOIK M E, et al. Genetic variation in circadian regulation of nocturnal stomatal conductance enhances carbon assimilation and growth[J]. Plant cell environ, 2016, 39:3-11.
[8]	GARICA-PLAZAOLA J I,FERNANDEZ-MARIN B,FERRIO J P, et al. Endogenous circadian rhythms in pigment composition induce changes in photochemical efficiency in plant canopies[J]. Plant cell environ, 2017, 40:1153-1162.
[9]	HAYDON M J, MIELCZAREK O, ROBERTSON F C, et al.Photosynthetic entrainment of the Arabidopsis thaliana circadian clock[J]. Nature,2013,502:689-692.
[10]	KO D K,ROHOZINSKI D,SONG Q,et al.Temporal shift of circadian-mediated gene expression and carbon fixation contributes to biomass heterosis in maize hybrids[J]. Plosgenet,2016,12:e1006197.
[11]	NAKAMICHI N, KIBA T, KAMIOKA M, et al.Transcriptional repressor PRR5 directly regulates clock-output pathways[J]. Proc natlacadsci USA, 2012, 109:17123-17128.
[12]	TODA Y, KUDO T, KINOSHITA T, et al.Evolutionary insight into the clock-associated PRR5 transcriptional network of flowering plants[J]. Sci Rep, 2019, 9:2983.
[13]	NAKAMICHI N, KIBA T, HENRIQUES R, et al.PSEUDO-RESPONSE REGULATORS 9,7, and 5 are transcriptional repressors in the Arabidopsis Circadian Clock[J]. Plant cell,2010,22:594-605.
[14]	SATO E, NAKAMICHI N, YAMASHINO T, et al.Aberrant expression of the Arabidopsis circadian-regulated APRR5 gene belonging to the APRR1/TOC1 quintet results in early flowering and hypersensitiveness to light in early photomorphogenesis[J]. Plant cell physiol, 2002, 43:1374-1385.
[15]	YAMAMOTO Y, SATO E, SHIMIZU T, et al.Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time,and early photomorphogenesis[J]. Plant cell physiol,2003,44:1119-1130.
[16]	NAKAMICHI N, KITA M, ITO S, et al.PSEUDO-RESPONSE REGULATORS, PRR9, PRR7 and PRR5, together play essential roles close to the circadian clock of Arabidopsis thaliana[J]. Plant cell physiol, 2005, 46:686-698.
[17]	RUTS T,MATSUBARA S, WIESE-KLINKENBERG A, et al.Aberrant temporal growth pattern and morphology of root and shoot caused by a defective circadian clock in Arabidopsis thaliana[J]. Plant J, 2012, 72:154-161.
[18]	MATSUSHIKA A,MAKINO S,KOJIMA M, et al.Circadian waves of expression of the APRR1/TOC1 family of Pseudo-Response Regulators in Arabidopsis thaliana: Insight into the plant circadian clock[J]. Plant cell physiol, 2000, 41:1002-1012.
[19]	NOHALES MA, KAY SA.Molecular mechanisms at the core of the plant circadian oscillator[J]. Nat Struct Mol Biol,2016,23:1061-1069.
[20]	XIE Q, WANG P, LIU X, et al.LNK1 and LNK2 are transcriptional coactivators in the Arabidopsis circadian oscillator[J]. Plant cell, 2014, 26:2843-2857.
[21]	KIBA T, HENRIQUES R, SAKAKIBARA H, et al.Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana[J]. Plant cell, 2007, 19:2516-253.
[22]	LIU Z,AN L,LIN S, et al.Comparative physiological and transcriptomic analysis of pear leaves under distinct training systems[J]. Sci Rep, 2020, 10:18892.
[23]	LIU Z, CHENG K, QIN Z, et al.Selection and validation of suitable reference genes for qRT-PCR analysis in pear leaf tissues under distinct training systems[J].PLos one,2018,13(8):e0202472.

PpPRR5基因在不同光合速率梨品种中的表达分析

Expression analysis of PpPRR5 gene in pear genotypes with different degrees of photosynthetic rate

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 23

相关文章 11

编辑推荐

Metrics

本文评价

[1]	杨小菊, 杨胜优, 罗倩, 魏芳怡, 龙雪燕, 廖婷婷, 王佳乐, 胡玉玲. 不同品种油茶果大小对其经济性状的影响[J]. 湖北农业科学, 2023, 62(6): 114-119.
[2]	徐芬, 郭红岩, 喻小兵, 彭立军, 姚晶晶. CO₂浓度升高和有机肥-化肥施用对水稻光合作用、抗氧化酶活性和重金属含量的影响[J]. 湖北农业科学, 2023, 62(12): 51-55.
[3]	郑艳萍, 杜亮亮, 宋晓辉, 王冠, 石建丰, 郭金海. 迁西梨树花期预报及梨花观赏旅游气象指数研究[J]. 湖北农业科学, 2023, 62(1): 108-111.
[4]	周建云, 余佳斌, 刁朝强, 覃青青, 王晓. 干旱胁迫下木醋液对烤烟光合特性及根系生长的影响[J]. 湖北农业科学, 2022, 61(4): 85-88.
[5]	陈广侠, 孔金花, 孔海明, 杨煜, 刘芳, 杨元军, 郭晓, 杨晓慧, 董道峰. 不同钾肥对马铃薯产量、品质、光合作用以及经济效益的影响[J]. 湖北农业科学, 2021, 60(24): 59-62.
[6]	林兵, 武胜利, 管文轲, 宋川, 史智欣, 何宇翔, 岳永江. 胡杨叶片的胞间CO₂浓度及气孔和非气孔限制的探究[J]. 湖北农业科学, 2021, 60(13): 87-92.
[7]	张媛媛. 光敏色素的结构及其信号调控机制[J]. 湖北农业科学, 2020, 59(4): 5-9.
[8]	王晶晶, 陈奇凌, 李铭, 郑强卿. 施肥量对滴灌骏枣光合作用、产量及品质的影响[J]. 湖北农业科学, 2020, 59(11): 39-42.
[9]	王丽霞, 黄大野, 林妃, 殷晓敏, 何应对, 王必尊. 外源硒对福橙叶片光合特性和叶片养分含量的影响[J]. 湖北农业科学, 2019, 58(5): 61-66.
[10]	汪本福, 王晴芳, 李阳, 张枝盛, 杨晓龙, 李汉东, 程建平. 干旱胁迫对水稻叶片生理生化特性的影响综述[J]. 湖北农业科学, 2019, 58(23): 5-5.
[11]	何春, 覃德文, 邓殷, 刘军, 梁燕芳, 秦武明. 遮阴处理对香子楠幼苗生长特性的影响[J]. 湖北农业科学, 2018, 57(1): 74-77.