湖北农业科学 ›› 2022, Vol. 61 ›› Issue (19): 5-10.doi: 10.14088/j.cnki.issn0439-8114.2022.19.001

• 育种·栽培 •    下一篇

一种快速灵敏的水稻种子老化寿命评估方法

郭彦丽, 佟卉, 刘燕清, 路信, 张红磊, 闫双勇, 张融雪, 苏京平, 刘学军   

  1. 天津市农业科学院作物研究所/天津市农作物遗传育种重点实验室,天津 300384
  • 收稿日期:2021-11-24 出版日期:2022-10-10 发布日期:2022-11-04
  • 通讯作者: 刘学军,主要从事水稻遗传育种,(电子信箱)sdlxj818@163.com。
  • 作者简介:郭彦丽(1984-),女,河南濮阳人,助理研究员,主要从事水稻种质资源创制与新品种选育研究,(电话)13297008856(电子信箱)feixueguoyanli@163.com。
  • 基金资助:
    天津市农业科学院青年科技创新项目(2020011); 天津市水稻产业技术体系项目(ITTRRS2021100000); 小站稻品种优质绿色丰产增效技术集成示范项目(202101050)

A rapid and sensitive method for evaluating aging life of rice seeds

GUO Yan-li, TONG Hui, LIU Yan-qing, LU Xin, ZHANG Hong-lei, YAN Shuang-yong, ZHANG Rong-xue, SU Jing-ping, LIU Xue-jun   

  1. Tianjin Key Laboratory of Crop Genetics and Breeding, Tianjin Crop Research Institute, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
  • Received:2021-11-24 Online:2022-10-10 Published:2022-11-04

摘要: 以9个水稻品种(塞卡、IR30、Bra80sci9875、越光、港源、日本晴、金粳818、金稻919、津稻372)种子为试验材料,采用不同温度、不同时间水浴处理,置于人工气候培养室内浸种总时间为24 h后进行发芽试验,测定种子发芽率、根长、芽长和活力。结果表明,当处理温度为50 ℃时,5个水稻品种(塞卡、越光、港源、日本晴、津稻372)平均发芽率有明显差异,日本晴达到G50 的品种比例为60.00%,可以准确区分不同水稻品种的发芽率;随着水浴时间的延长,水稻品种的平均发芽率均呈下降趋势,其中津稻372的老化速度最快,处理 4 h其平均发芽率几乎降为0。处理4 h时,达到G50的水稻品种超过50.00%,9个水稻品种的平均发芽率分布比较均匀,依次为塞卡>越光>日本晴>金稻919>IR30>港源>Bra80sci9875>金粳818>津稻372。在50 ℃,4 h水浴条件下进行的加速老化测试是一种快速而灵敏的方法,可以在7 d内完成。

关键词: 温度, 时间, 发芽, 种子活力

Abstract: The seeds of 9 rice varieties (Saika, IR30, Bra80sci9875, Koshihikari, Gangyuan, Nipponbare, Jinjing 818, Jindao 919, Jindao 372) were used as experimental materials. The seeds were treated with water bath at different temperatures and different times. The germination test was carried out after soaking in the artificial climate culture room for 24 h. The germination rate, root length, bud length and vigor were measured. The results showed that when the treatment temperature was 50 ℃, the average germination rate of five rice varieties (Saika, Koshihikari, Gangyuan, Nipponbare, Jindao 372) was significantly different, and the proportion of Nipponbare varieties reaching G50 was 60.00%, which could accurately distinguish the germination rate of different rice varieties. With the extension of water bath time, the average germination rate of rice varieties showed a downward trend. The aging rate of Jindao 372 was the fastest, and the average germination rate was almost 0 after 4 h treatment. When treated for 4 h, more than 50.00% of the rice varieties reached G50, and the average germination rate distribution of the nine rice varieties was relatively uniform, with the order of Saika>Koshihikari>Nipponbare>Jindao 919>IR30>Gangyuan>Bra80sci 9875>Jinjing 818>Jindao 372. Accelerated aging testing at 50 ℃ for 4 h in a water bath is a fast and sensitive method that can be completed within 7 days.

Key words: temperature, time, germination, seed vigor

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