湖北农业科学 ›› 2022, Vol. 61 ›› Issue (21): 147-151.doi: 10.14088/j.cnki.issn0439-8114.2022.21.028

• 检测分析 • 上一篇    下一篇

山茶油掺入四种植物油的快速鉴别

黄更生1a, 阮媚1a, 陈兴发1b, 卜丹2, 穆洪涛1a   

  1. 1.广东第二师范学院,a.生物与食品工程学院;b.数学学院,广州 510303;
    2.广州华侨医院,广州 510630
  • 收稿日期:2021-10-01 出版日期:2022-11-10 发布日期:2022-12-10
  • 作者简介:黄更生(1970-),男,湖南武冈人,讲师,硕士,主要从事生物技术研究,(电话)13710657765(电子信箱)huanggs@gdei.edu.cn。

Rapid identification of camellia oil mixed with four vegetable oils

HUANG Geng-sheng1a, RUAN Mei1a, CHEN Xing-fa1b, BU Dan2, MU Hong-tao1a   

  1. 1. a. College of Biological and Food Engineering; b. School of Mathematics, Guangdong University of Education, Guangzhou 510303,China;
    2. Guangzhou Overseas Chinese Hospital, Guangzhou 510630, China
  • Received:2021-10-01 Online:2022-11-10 Published:2022-12-10

摘要: 为鉴别山茶油是否掺假,基于前表面荧光技术对山茶油掺入4种其他植物油进行荧光扫描,分别以5%、10%、20%、30%、40%为体积梯度,制备不同的山茶油样品,利用前4个主成分建立山茶油掺假判别模型。结果表明,山茶油与其他4种植物油的荧光特性存在显著差异;在激发波长250~380 nm,发射波长260~550 nm处,采用前表面荧光技术进行主成分分析,前4个主成分的总贡献率大于85%;通过Fisher判别分析法进一步分析,其对山茶油掺假识别率高达100%。该方法可实现对山茶油的定性和定量分析,且具有较高的灵敏度和快速的特点。

关键词: 山茶油, 检测, 前表面荧光技术, PCA, Fisher判别分析

Abstract: In order to identify the adulteration of camellia oil, the fluorescence scanning of camellia oil mixed with four other vegetable oils was carried out based on the Front surface fluorescence technology. Different camellia oil samples were prepared with 5%, 10%, 20%, 30% and 40% as the volume gradient, respectively. The first four principal components were used to establish the discrimination model of camellia oil adulteration. The results showed that there were significant differences in fluorescence characteristics between camellia oil and the other four vegetable oils. Under the excitation wavelength of 250~380 nm and the emission wavelength of 260~550 nm, the principal component analysis was carried out by Front surface fluorescence. The total contribution rate of the first four principal components was more than 85%. Through further analysis of Fisher discriminant analysis, the adulteration recognition rate of camellia oil was as high as 100%. This method could realize the qualitative and quantitative analysis of camellia oil, and had high sensitivity and fast characteristics.

Key words: camellia oil, detection, Front surface fluorescence technology, PCA, Fisher discriminant analysis

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