荒漠肉苁蓉总低聚糖提取工艺优化及生物活性分析

doi:10.14088/j.cnki.issn0439-8114.2025.02.024

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (2): 156-161.doi: 10.14088/j.cnki.issn0439-8114.2025.02.024

荒漠肉苁蓉总低聚糖提取工艺优化及生物活性分析

田占萍¹, 王宇¹, 吉舒仪¹, 谭峻杰¹, 姚军¹, 沈静^2,3

1.新疆医科大学药学院,乌鲁木齐 830017;
2.新疆医科大学第一附属医院药学部,乌鲁木齐 830054;
3.新疆医科大学第五附属医院药学部,乌鲁木齐 830011

收稿日期:2024-07-03 出版日期:2025-02-25 发布日期:2025-03-07
通讯作者: 沈静（1977-）,女,新疆乌鲁木齐人,教授,博士,主要从事药物分析研究,（电话）18119185606（电子信箱）6572177@qq.com。
作者简介:田占萍（1997-）,女,青海西宁人,在读硕士研究生,研究方向为药物分析,（电话）17590938691（电子信箱）1413251663@qq.com。
基金资助:
新疆维吾尔自治区重大科技专项项目（2022A03007-2）; 新疆维吾尔自治区自然科学基金项目（2022D01C327）

Optimization of extraction process and biological activity analysis of total oligosaccharides from Cistanche deserticola

TIAN Zhan-ping¹, WANG Yu¹, JI Shu-yi¹, TAN Jun-jie¹, YAO Jun¹, SHEN Jing^2,3

1. School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China;
2. Department of Pharmacy, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;
3. Department of Pharmacy, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China

Received:2024-07-03 Published:2025-02-25 Online:2025-03-07

摘要/Abstract

摘要： 采用超声辅助法提取荒漠肉苁蓉（Cistanche deserticola）总低聚糖,通过单因素和正交试验优化提取条件;运用红外光谱初步解析荒漠肉苁蓉总低聚糖的结构特征;通过测定α-葡萄糖苷酶的抑制能力、DPPH、·OH及ABTS自由基的清除率研究荒漠肉苁蓉总低聚糖的体外降糖能力和体外抗氧化活性。结果表明,荒漠肉苁蓉总低聚糖的最佳提取工艺条件为料液比1∶50,乙醇浓度40%,超声时间32 min,在该条件下总低聚糖得率达17.32%;红外光谱图表明有明显的总低聚糖特征吸收,且为β-型吡喃糖类;生物活性结果显示对α-葡萄糖苷酶的抑制率达63.94%,对DPPH、·OH及ABTS自由基清除率分别达85.63%、60.91%、82.90%。该方法有助于提高荒漠肉苁蓉总低聚糖得率,且其具有较强的体外抗氧化和降糖能力,可为相关研究提供参考。

关键词: 荒漠肉苁蓉（Cistanche deserticola）, 总低聚糖, 超声辅助法提取, 抗氧化, 降血糖

Abstract: The total oligosaccharides from Cistanche deserticola were extracted by ultrasonic-assisted method, and the extraction condition was optimized by single factor and orthogonal test. The structural characteristics of total oligosaccharides of Cistanche deserticola were preliminarily analyzed by infrared spectroscopy. The in vitro hypoglycemic ability and antioxidant activity of total oligosaccharides from Cistanche deserticola were studied by measuring the inhibition ability of α-glucosidase, and the scavenging rate of DPPH, ·OH and ABTS free radicals. The results showed that the optimum extraction condition of total oligosaccharides from Cistanche deserticola was the solid-liquid ratio of 1∶50, ethanol concentration of 40% and ultrasonic time of 32 min. Under this condition, the yield of total oligosaccharides was 17.32%. The infrared spectroscopy showed that there was obvious characteristic absorption of total oligosaccharides, and it was β-pyranose. The results of biological activity showed that the inhibition rate of α-glucosidase was 63.94%, and the scavenging rates of DPPH, ·OH and ABTS free radicals were 85.63%, 60.91% and 82.90%, respectively. This method was helpful to improve the yield of total oligosaccharides from Cistanche deserticola, and these total oligosaccharides had strong antioxidant and hypoglycemic ability in vitro, which could provide reference for related research.

Key words: Cistanche deserticola, total oligosaccharides, ultrasonic assisted extraction, antioxidation, hypoglycemic

中图分类号:

R284.2

田占萍, 王宇, 吉舒仪, 谭峻杰, 姚军, 沈静. 荒漠肉苁蓉总低聚糖提取工艺优化及生物活性分析[J]. 湖北农业科学, 2025, 64(2): 156-161.

TIAN Zhan-ping, WANG Yu, JI Shu-yi, TAN Jun-jie, YAO Jun, SHEN Jing. Optimization of extraction process and biological activity analysis of total oligosaccharides from Cistanche deserticola[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 156-161.

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荒漠肉苁蓉总低聚糖提取工艺优化及生物活性分析

Optimization of extraction process and biological activity analysis of total oligosaccharides from Cistanche deserticola

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