高产花生品种开农80的耐盐性鉴定及机制解析

doi:10.14088/j.cnki.issn0439-8114.2025.06.005

摘要/Abstract

摘要： 以杂交花生（Arachis hypogaea）品种开农80为材料,鉴定其耐盐性及相关分子机制。结果显示,区域试验产量测定中,与对照品种豫花9327相比,开农80荚果产量显著增加。萌发期在90 mmol/L NaCl胁迫下,开农80种子中AhNCED1表达显著下调,AhCYP707A1和AhCYP707A2表达显著上调,导致脱落酸（ABA）含量降低;AhGA2ox表达显著下调但AhGA3ox表达显著上调,导致赤霉素（GA）含量升高,因此开农80在盐胁迫下的发芽势、发芽率和发芽指数均显著高于对照品种豫花9327。幼苗期在200 mmol/L NaCl胁迫下,与对照品种豫花9327相比,开农80中活性氧清除酶的活性显著提高,过氧化氢和超氧阴离子的含量显著降低,因而开农80幼苗的根数显著增加,脯氨酸和叶绿素含量显著升高,而丙二醛含量显著降低,存活率显著提高。

关键词: 花生（Arachis hypogaea）, 开农80, 高产, 耐盐性, 赤霉素, 脱落酸, 基因表达, 活性氧

Abstract: The salt tolerance and underlying molecular mechanisms of the hybrid peanut (Arachis hypogaea) cultivar Kainong 80 were systematically investigated. The results revealed that in regional yield trials, the pod yield of Kainong 80 was significantly higher than that of the control variety Yuhua 9327. Under 90 mmol/L NaCl stress during germination, the expression of AhNCED1 in Kainong 80 seeds was markedly down-regulated, while the expression of AhCYP707A1 and AhCYP707A2 was significantly up-regulated, resulting in a reduction in abscisic acid content. Additionally, the expression of AhGA2ox was sharply down-regulated, whereas the expression of AhGA3ox was significantly up-regulated, leading to an increase in gibberellin content. Consequently, the germination vigor, germination rate, and germination index of Kainong 80 under salt stress were significantly higher than those of the control variety Yuhua 9327. Under 200 mmol/L NaCl stress at the seedling stage, compared with the control variety Yuhua 9327,the activity of reactive oxygen species scavenging enzymes in Kainong 80 was significantly increased, and the contents of hydrogen peroxide and superoxide anion were significantly decreased. Therefore, the number of roots of Kainong 80 seedlings was significantly increased, the contents of proline and chlorophyll were significantly increased, while the content of malondialdehyde was significantly decreased, and the survival rate was significantly increased.

Key words: peanut (Arachis hypogaea), Kainong 80, high yield, salt tolerance, gibberellin, abscisic acid, gene expression, reactive oxygen species

中图分类号:

S565.2

王培云, 赵文丽, 马骥, 邓丽, 李阳, 郭敏杰, 申卫国, 姚潜, 任丽. 高产花生品种开农80的耐盐性鉴定及机制解析[J]. 湖北农业科学, 2025, 64(6): 21-27.

WANG Pei-yun, ZHAO Wen-li, MA Ji, DENG Li, LI Yang, GUO Min-jie, SHEN Wei-guo, YAO Qian, REN Li. Identification and mechanism analysis of salt tolerance in high-yield peanut variety Kainong 80[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 21-27.

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