湖北农业科学 ›› 2026, Vol. 65 ›› Issue (7): 105-109.doi: 10.14088/j.cnki.issn0439-8114.2026.07.016

• 植物保护 • 上一篇    下一篇

基于三油酸甘油酯的植物源农药研制及室内效果评价

史棋文1a,2, 高晶2, 叶凤娴2, 王朝红2, Clement Nzabanita2, 吴仲真1b, 毛润乾2   

  1. 1.仲恺农业工程学院,a.资源与环境学院; b.农业与生物学院, 广州 510225;
    2.广东省科学院动物研究所/广东省动物保护与资源利用重点实验室/广东省矿物油农药工程技术研究中心,广州 510260
  • 收稿日期:2026-03-02 出版日期:2026-07-25 发布日期:2026-07-23
  • 通讯作者: 吴仲真(1984-),男,副教授,博士,主要从事植物与昆虫互作研究,(电子信箱)zhongzhen_wu@163.com。
  • 作者简介:史棋文(1997-),男,江西吉安人,在读硕士研究生,研究方向为植物保护,(电子信箱)shiqiwen2023@163.com;共同第一作者,高 晶(1989-),女,河南永城人,副研究员,博士,主要从事植物保护研究,(电子信箱)gaoj@giz.gd.cn;毛润乾(1968-),男,研究员,博士,主要从事植物保护研究,(电子信箱)maorun@giz.gd.cn。
  • 基金资助:
    广东省科学院发展专项资金项目(2024GDASZH-2024010101; 2024GDASZH-2024010102); 广东省科学院青年人才专项(2024GDASQNRC-0315); 外国专家项目(Y20240128); 广东省普通高校创新团队项目(2025KCXTD022)

Development of a triolein-based botanical pesticide and its laboratory efficacy evaluation

SHI Qi-wen2,1a, GAO Jing2, YE Feng-xian2, WANG Zhao-hong2, Clement Nzabanita2, WU Zhong-zhen1b, MAO Run-qian2   

  1. 1a. College of Resources and Environment; 1b. College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    2. Institute of Zoology/Guangdong Key Laboratory of Animal Conservation and Resource Utilization/Guangdong Engineering Research Center for Mineral Oil Pesticides, Guangdong Academy of Sciences, Guangzhou 510260, China
  • Received:2026-03-02 Published:2026-07-25 Online:2026-07-23

摘要: 以植物油的主要活性成分三油酸甘油酯为对象,开发一种新型绿色农药,并评价其制剂性能、杀虫活性及对作物的生长调节作用。通过乳化剂筛选与复配,确定最佳亲水亲油平衡值(HLB),并优化乳化体系,制备90%三油酸甘油酯乳剂;采用浸渍法评价其对烟粉虱(Bemisia tabaci)成虫的触杀毒力;利用Y形嗅觉仪检测其驱避活性;通过盆栽试验研究其对番茄幼苗生长的促进效应。结果表明,三油酸甘油酯的最佳HLB为8.5。由AEO3、NP4与Tween-80(质量比1∶1∶0.015)组成的复合乳化剂在10%添加量下,可形成乳化效果优异的乳剂。该乳剂对烟粉虱成虫表现出显著触杀活性,LC50为167.16 mg/L,毒力显著高于对照SK矿物油(LC50=354.54 mg/L)。同时,其对烟粉虱具有驱避作用,施药后1 h和1 d的驱避率分别为76.00%和64.37%,各时间点的驱避率与对照SK矿物油均无显著差异。浓度为5.00 mg/L的三油酸甘油酯能持续促进番茄幼苗生长,在多次处理后,其株高相对生长速率和茎粗净生长量均显著高于空白对照(CK);而相同浓度的SK矿物油则对番茄茎粗增长表现出显著的抑制作用。

关键词: 三油酸甘油酯, 植物源农药, 乳化剂, 烟粉虱(Bemisia tabaci), 番茄

Abstract: Using triolein, the main active component of vegetable oils, as the target compound, a novel green pesticide was developed, and its formulation performance, insecticidal activity, and growth-regulating effects on crops were evaluated. The optimal hydrophilic-lipophilic balance (HLB) was determined through emulsifier screening and compounding, and the emulsification system was optimized to prepare a 90% triolein emulsion. The toxicity of the emulsion against adult whiteflies (Bemisia tabaci) was evaluated using the immersion method, its repellent activity was tested with a Y-tube olfactometer, and its growth-promoting effects on tomato seedlings were investigated through pot experiments. The results showed that the optimal HLB for triolein was 8.5. A composite emulsifier consisting of AEO3, NP4, and Tween-80 at a mass ratio of 1∶1∶0.015, added at a concentration of 10%, produced an emulsion with excellent emulsification properties. The emulsion exhibited significant contact toxicity against adult whiteflies, with an LC50 of 167.16 mg/L, which was significantly lower than that of the control SK mineral oil (LC50 = 354.54 mg/L). In addition, the emulsion showed a repellent effect against whiteflies, with repellency rates of 76.00% and 64.37% at 1 hour and 1 day after application, respectively, and no significant differences were observed between the triolein emulsion and the SK mineral oil control at any time point. At a concentration of 5.00 mg/L, triolein consistently promoted the growth of tomato seedlings, with both the relative growth rate of plant height and the net increase in stem diameter being significantly higher than those of the blank control after multiple treatments. In contrast, the same concentration of SK mineral oil significantly inhibited the net increase in tomato stem diameter.

Key words: triolein, botanical pesticide, emulsifier, Bemisia tabaci, tomato

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